Wash & Blot Stations for Robotic Pin Tools

 


  Cleaning Pins On Robot Mounted Replicators

 
   
Static Reservoirs
Disposable Reservoirs
96 Fountain
384 Fountain
Spillway Reservoirs
Drain & Fill Ports
Dual Chamber
Pin Cleaning
Pin Cleaning Data 1
Pin Cleaning Data 2
Peristaltic Pump Kit
PTFE Reservoirs
"Holey" Blotting Paper
"Super" Blotting Paper


The most important aspect of using pin tools is to start with clean pins and then keep them clean so they will consistently deliver the same volume.  We recommend our Pin Cleaning Solution (VP 110) to clean the pin of protein and other residue buildup before you start an assay.  To quickly and efficiently remove the previous transfer from the pin we have several different wash reservoirs, flowing reservoirs and fountains to clean  96, 384 and 1536 floating pin tools.  These wash stations also are adapted so the blotting station is part of the washing station thus reducing the cleaning cycle time and the number of robot stations required for the cleaning process.  We also have other independent blotting stations (VP 540D)

 

Integral to the cleaning process of all pins is the blotting step between baths.  Lint particles in the slots or on pin tips, from standard paper towels, will interfere with the loading and unloading of liquid.  We have solved this problem.  The VP 540DB blotting system is a tray with the standard microplate foot print that contains a super absorbent polypropylene pad covered by a Lint Free Blotting Paper.  We strongly recommend that you use this or our other lint free blotting systems.

 

For chemical resistance properties of Polypropylene and other commonly used plastics follow this link.


Click here for a list of published articles that cite the products on this page.

 

Movie of washing the pins

 
 


We have developed detailed procedures for cleaning pin tools on robot systems.

 

Data demonstrating thoroughness of wash procedure on  carry over of compound after 100 transfers with a slot pin.

 


  Combination Single Reservoir Blotting Station

   
 


Pin tools must be washed in 2 liquid reservoirs between different source plates so the pins don't contaminate the next source plate they are placed into.  This process can be streamlined and simplified using our unique Combination Reservoir and Blotting System (Patent pending).  Combining the blotting station on top of the wash reservoir not only reduces the cycle time but more importantly reduces the number of precious robot station positions that are needed to clean the pins from 3 to 2.  

   
 


The VP 540-1 Reservoir

   
 


The VP 540-1 Reservoir is available for use with both 96 hole (VP 540A) and 384 hole (VP 540B1) blotting media supports.  96 hole lint free blotting media (VP 540AC) is positioned on top of the VP 540A support  and the 384 hole lint free blotting media (VP 540BC) is positioned on top of the VP 540B1 support.  We also offer a system of removable baffles (VP 540F-1 for the VP 540-1 reservoir) to reduce wave action in moving reservoirs.  If your robot system moves the wash reservoir around during the washing cycle you will find this a very useful feature.  The baffles are made from the autoclavable epoxy composite G10. 

* If you are having difficulty using your VP 540AC paper when performing a wash and blot on the CyBio Robot click here for more information.

Most of our reservoirs are made from rugged polypropylene.  Polypropylene is the first choice in resistance to organic solvents, acids and bases.  For a complete table on chemical and solvent resistance click here.

The VP 540-1 is constructed of autoclaveable polypropylene to fit into the SBS standard foot print with dimensions of 127 mm long, 85 mm wide and 32 mm tall with a cavity 24 mm deep.  The reservoir will hold 195 ml of wash fluid.  For robot platforms that require a shorter reservoir we offer the VP 542 which has the same footprint as the VP 540-1 but is only 15 mm tall.  This shorter version also has shorter baffles (VP 542F) strategically located to dampen wave action upon lateral movement.  The blotting media supports (VP 540A and VP 540B1) will fit on both reservoirs and are constructed using the autoclaveable epoxy composite G10.   The pin holes in the VP 540A supports are 6.39 mm in diameter and the holes in the VP 540B1 supports are 2.24 mm in diameter.

 


 
 


The photos to the right show the VP 540-1 wash station being used on an Apricot Personal Pin Tool Robot.  The second photo shows the combination single reservoir blotting station being used.

 

 


The VP 540L Reservoir

   
 

The VP 540L is constructed of autoclaveable polypropylene to fit into the SBS standard foot print with dimensions of 127 mm long, 85 mm wide and 32 mm tall with a cavity 25 mm deep.  The reservoir will hold 215 ml of wash fluid.  This reservoir was designed for applications on carousel robotic decks where rotational movement of the deck will set up a wave motion that will cause the reservoir contents to be "sloshed" out of the reservoir. The VP 540L has notches machined into the ends of the reservoir so the longer VP 540LF baffles can be placed parallel and thus prevent sloshing.   The blotting media supports (VP 540A and VP 540B1) will fit on these reservoirs. 


The VP 540LF Baffles are constructed using the autoclaveable epoxy composite G10.

 
 
 


The VP 540L-PTFE is constructed of autoclaveable PTFE to fit into the SBS standard foot print with dimensions of 127 mm long, 85 mm wide and 32 mm tall with a cavity 25 mm deep.  The reservoir will hold 215 ml of wash fluid.  This reservoir was designed for applications on carousel robotic decks where rotational movement of the deck will set up a wave motion that will cause the reservoir contents to be "sloshed" out of the reservoir. The VP 540L_PTFE has notches machined into the ends of the reservoir so the longer VP 540LF-PTFE baffles can be placed parallel and thus prevent sloshing.   The blotting media supports (VP 540A and VP 540B1) will fit on these reservoirs.
 

The VP 540LF-PTFE baffles are constructed of anodized aluminum that is coated with PTFE.

 


The VP 540L-1536  reservoir is  constructed of aluminum for precision dimensioning.   Because of the tight dimensional tolerance associated with 1536 pin tools we designed this reservoir with very thin baffles to fit between pins on 2.25 mm centers.   This reservoir was designed for applications on carousel robotic decks where rotational movement of the deck will set up a wave motion that will cause the reservoir contents to be "sloshed" out of the reservoir. The VP 540L-1536 has 3 sets of notches machined into the ends of the reservoir so the VP 540LF-1536 baffles can be placed parallel and thus prevent sloshing. The reservoir is precision dimensioned to fit into the SBS standard foot print (127 mm long, 85 mm wide and 32 mm tall with a cavity 25 mm deep).  The reservoir will hold 240 ml of wash fluid.  The VP 540L-1536 has notches machined into the ends of the reservoir so the VP 540LF-1536 baffles can be placed parallel and thus prevent sloshing. 
 

The VP 540L-1536, reservoir and VP 540LF-1536 baffles are both constructed of aluminum for dimensional precision.  The aluminum is coated with Ni-Lube for corrosion resistance (Submicron particles of Teflon are co-deposited with an electroless Nickel-Phosphorus alloy)

 

The VP 542 Reservoir
   
 


The VP 542 is constructed of autoclaveable polypropylene to fit into the SBS standard foot print with dimensions of 127 mm long, 85 mm wide and 15 mm tall with a cavity 12.5 mm deep.  The reservoir will hold 95 ml of wash fluid.  The VP 542 reservoir also has shorter baffles (VP 542F) strategically located to dampen wave action upon movement.  The blotting media supports (VP 540A and VP 540B1) will fit on both reservoirs and are constructed using the autoclaveable epoxy composite G10.

 
 


The VP 540PCB Reservoir

   
 


The VP 540PCB is constructed of solvent resistant PTFE to fit into the Cybi Well Robot plate holders. The dimensions are 140 mm long, 102.3 mm wide and 32 mm tall with a cavity 26 mm deep.  The reservoir will hold 200 ml of wash fluid.  The VP 540PCB reservoir also is able to use the standard VP 540F baffles.  When the baffles are in place they dampen the wave action that occurs upon plate movement.  The baffles are constructed using the autoclaveable epoxy composite G10.

Disposable Wash Reservoirs
We also have disposable polypropylene wash reservoirs for your convenience.  Some of our disposable reservoirs are taller and some are shorter than our reusable reservoirs.  In cases where clearance issues are important, one may have advantages over the other.

 
 


VP 540T

     
 


Single polypropylene wash reservoir with 13 baffles, 127.76mm long, 85.48mm wide, 32mm tall, 25mm deep reservoir, alignment pins protrude 1.5mm and female luer 5/16 hex to 1/4 -28 UNF thread fill and drain connections.

 
 
 


The photo to the right shows a custom VP 540T retrofitted with large diameter nipples to allow for larger throughput of liquid.

 
 
     
 
 


Outlet and Inlet Connectors

   
 


Some of the reservoirs (VP 540T and VP 542T) are available with outlet and inlet connectors.  This facilitates filling and draining the reservoir on the work station between runs.

   

 
   Combination Dual Chamber Reservoir Blotting Station

 
 


Pin tools must be washed in two liquid reservoirs between different source plates so the pins don't contaminate the next source plate they are placed into. This process can be streamlined and simplified using our unique Combination Dual Chamber Wash and Blotting System (Patent pending).  Combining the blotting station on top of dual wash chambers not only reduces the cycle time but more importantly reduces the number of precious robot station positions that are needed to clean the pins from three to one.

 

 
 


The Dual Chamber Model

   
 


VP 541

     
 


The VP 541 is available for use with only 96 hole (VP 540A)  blotting media supports.  96 hole lint free blotting media (VP 540AC) is also used with this system. 

The dual chamber model (VP 541) is constructed of delrin and designed  to fit into the SBS standard foot print with dimensions of 127 mm long, 85 mm wide and 32 mm tall with two interdigitating chambers 25 mm deep.  Each chamber will hold 70 ml of wash fluid.  The interdigitation design (on 4.5 mm centers) of the dual chamber eliminates the need for wave dampening baffles.  The blotting media support VP 540A are constructed using the autoclaveable epoxy composite G10.  The reservoir is not autoclaveable.

An outlet drain and fill version of the reservoir (VP 541T) is also available.  This facilitates filling and draining the dual reservoirs while on the work station between runs.

 

 

 

 
   Combination Single Fountain Wash and Blot Station

 
 


Pin tools must be washed in two static liquid reservoirs or one fountain wash station  between different source plates so the pins don't contaminate the next source plate they are placed into.  This process can be streamlined and simplified using our unique Combination Fountain and Blotting System (Patent pending).  Combining the blotting station on top of the wash fountain not only reduces the cycle time but more importantly reduces the number of precious robot station positions that are needed to clean the pins from 3 to 2 or 3 to 1. 

 
 
 


The 96 Fountain Model VP 545

   
 


The VP 545 is available for use with a 96 hole (VP 540A) blotting media support.  96 hole lint free blotting media (VP 540AC) is positioned on top of the VP 540A support.    The wash station has a drain and a fountain outlet that can be connected to a peristaltic pump to drain the reservoir and push wash fluids through the fountains.  We recommend a cleaning/wash procedure that incorporates a dip in the fountain of 99% isopropanol followed by a blot step and then another dip and blot in the same fountain.

 
 
 


The  384 Fountain Model VP 546

   
 


The VP 546  is available for use with a 384 hole (VP 540B1) blotting media support.   384 hole lint free blotting media (VP 540BC) is positioned on top of the VP 540B1 support.  The wash station has a drain and a fountain outlet that can be connected to a peristaltic pump to drain the reservoir and push wash fluids through the fountains.  We recommend a cleaning/wash procedure that incorporates a dip in the fountain of 99% isopropanol followed by a blot step and then another dip and blot in the same fountain.
 

Both the 96 and 384 wash fountains are constructed of autoclaveable PTFE and PTFE coated anodized aluminum and are designed to handle corrosive organic solvents.  These fountains are designed to simulate the footprint and locating holes of CyBio's Cybi Well microplate carrier (146 mm long by 104 mm wide and 22 mm tall).   The blotting media supports (VP 540A and VP 540B1) are constructed using the autoclaveable epoxy composite G10.
 

Pins are washed by slowly dipping them into the fountain holes and letting the isopropanol carry the residual material away.  The fountain holes are drilled through an angled surface so the effluent from each hole goes into gutters in between the column of fountains and the effluents are not able to mix until they are in the gutters.  This prevents the effluents of one pin from contaminating its neighbor. 

 
 

 


The photo on the right shows a Peristaltic Pump connected to a source wash solution and a waste collection trap.  By selecting a slightly larger internal diameter tubing for the drain you insure that the system will not over flow.

 
 


VP 560

     
 


For your convenience we have combined all the components necessary to operate the fountain into a Peristaltic Pump Kit VP 560.  The kit contains: peristaltic pump, computer control software, RS-232 cable, 25 feet of 6 mm ID Viton tubing, 25 feet of 8 mm ID Viton tubing, 3 pump heads and  mounting for 3 heads, and all connectors.  You can control the pump speed on and off from the robot computer with a simple program we developed.

 
 


New Foot Print 384 Fountain

     
 


VP 546A

     
 


Some of our customers asked for a footprint that was more compatible with the SBS standard plate size.  The result is the VP 546A.  It meets the 127 mm width requirement and fits onto most robot plate holders by just adjusting the width to 110.5 mm.  We also fitted this version fountain with popular and easy female luer fittings.

 

 

 

 

The VP 546A is 127 mm long by 110.5 mm wide and 25.4 mm tall).   Like the VP 546 the fountains are made from PTFE but they are cut flat.  The base is anodized aluminum and coated with PTFE.

 

 
 

 
  Technical Documents:
    CAD drawing of dimensions
 



The New
VP 546A1T

   
 


This is a 46.5 mm tall version of the VP 546A fountain.  It is designed to be able to wash the tips of the Caliper (Zymark) 384 fixed pin pipettor.  With its greater depth, the pins can be inserted deeper (25 mm) into the fountain for better cleaning.  The individual channels are 25 mm deep and it is a total of 33 mm to the bottom of the fountain reservoir.  Note that the top surface is pitched laterally so the effluent wash fluid drains to both sides for a more efficient flow.  Each fountain channel is also cut on a bevel to facilitate the effluent flow.

 

The VP 546A1T is 127 mm long and fits onto most robot plate holders by just adjusting the width to 110.5 mm.  We also fitted this version fountain with larger ID nipple fittings to accommodate large volume flows.  Over all height including the base is 46.5 mm.

 
 
 

 
  Technical Documents:
    CAD drawing of dimensions
 


New Luer Fittings for VP 546B

   
 


The VP 546B is similar to the VP 546 fountain except it has a taller base to accept female luer fittings. Like the VP 546 it is designed to simulate the footprint and locating holes of CyBio's Cybi Well microplate carrier.  It is 146 mm long by 104 mm wide and 25.4 mm tall).

 
 


  Custom

 
 


VP 546C-CB

     
 


The VP 546C-CB is a custom designed fountain wash reservoir made with very small inlet holes (0.5 mm diameter) to equalize the wash liquid flow in each of the fountains.  The unequal flow of wash liquid is a problem that affects all other fountain wash stations.  We also construct our VP 546C-CB using hydrophilic coated aluminum which also equalizes the flow of liquid over the rim of each fountain.  Fountains made of PTFE are very hydrophobic and liquids build up in the wells until one of the fountains over flows.  Most of the fluid that flows comes through that first to flow well.

 

 
 


The photo on the right illustrates how the VP 546C-CB works on a Cybio robot.

 
 
 


The photo on right illustrates how the manifold can be used as either a “right” or “left” handed version depending how the in let and outlet nipples are applied.

 
 
       
 

 
   Combination Dual Fountain Wash and Blot Stations

     
 


VP 547

     
 


For the ultimate in cleaning, Pin tools can be washed in 2  fountains between different source plates so the pins don't contaminate the next source plate they are placed into.  This process can be streamlined and simplified using our unique Combination Dual Fountain and Blotting System (Patent pending).  Combining the blotting station on top of the dual wash fountains not only reduces the cycle time but more importantly reduces the number of precious robot station positions that are needed to clean the pins from 3 to1. 

The 96 dual fountain model (VP 547) is available for use with a 96 hole (VP 545A) blotting media support.  96 hole lint free blotting media (VP 540AC) is positioned on top of the VP 545A support.   Each wash station has a drain and two fountain inlets that can be connected to a peristaltic pump to drain the reservoir and push two different wash fluids through the two different fountain systems. The individual fountains from the two fountain pairings are positioned close enough together to be accessible from the same access hole.

The dual wash fountain is constructed of anodized PTFE coated aluminum and are designed to handle corrosive organic solvents.  This dual fountain is 148 mm long by 97 mm wide and 200 mm tall.  The blotting media support (VP 540A) is constructed using the autoclaveable epoxy composite G10.

 

 

 
    Spillway Flowing Reservoir Wash System

 
 


VP 548

     
 


The VP 548 Spillway Flowing Reservoir Wash System offers several advantages.  First the wash liquid is injected into the reservoir through 8 inlet ports on the right side of the reservoir and the liquid fills the reservoir to a height of 12 mm before it runs over 8 V notches in the spillway and is collected in a separate reservoir connected to the drain.  This keeps the wash reservoir uniformly flushed with new liquid coming in one end and waste liquid flowing out the other.  It maintains the height of the liquid at a constant level regardless of the flow rate.  The spillway feature is also important in washing 1536 pin tools because the significant displacement of wash liquid by 1536 pins can raise the level of liquid in a fixed reservoir. 

Specifications:
Dimensions - 144.7 mm long by 85.48 mm wide, 28 mm tall
Reservoir depth = 15 mm
Reservoir volume = 120 ml

The flowing reservoir/ spillway system allows displaced liquid  to merely overflow the spillway and the reservoir is refilled when the  pin tool is removed.  The Spillway Flowing Reservoir Wash System also is more cost effective than the fountain style reservoirs.  If you don't need to have the pins washed individually this a very cost effective way to clean pins for runs of over 100 plates/washings.

 

 

 

   Spillway Reservoir Formats

 
 


VP 549ETOH-4 & VP 549H2O-4

     
 

Our VP 549ETOH-4 and VP 549H2O-4 Spillway Reservoirs are widely used reservoirs that keep the fluid height relatively constant while also being continuously refreshed throughout the procedure. The wash solution flows into the Reservoir through the inlet port, and the excess liquid flows over the spillway and is removed through the outlet port. The wash solution can be recirculated using a peristaltic pump, collected for reuse, or drained into a disposal system.

 
 
 

The Spillway Reservoirs have an SBS footprint (127.7 mm x 85.5 mm) and they are very reasonably priced. They are provided in 2 versions because the solutions typically used have different surface tensions. For ethanol, isopropanol, methanol and DMSO wash solutions, the VP 549ETOH-4 Reservoir is recommended. For aqueous wash solutions, the VP 549H2O-4 Reservoir should be used. See photos on the right for comparison of the spillways.

 
 
 

Specifications:

  • Dimensions = 127.76 mm long by 85.48 mm wide by 29.26 mm tall
  • Reservoir depth = 20.4 mm
  • Reservoir volume = 135 ml
  • Tube ID = 3/16 inch
     
 

The VP549ETOH-4 and VP 549H2O-4 are particularly useful for washing Pin Tool pins before, during, and/or after Pin Tool procedures. Both the VP 549ETOH-4 and VP 549H2O-4 Reservoirs will accommodate any format that is SBS-dimensioned or smaller, including 1536 Pin Tools or pipette tips. Another advantage relates to the displacement of wash solution by the pins during washing and loss of that solution over the spillway. The Spillway Reservoirs are filled automatically when the pins are removed.

     
 

Click here for a video of a Biomek Robot using our Spillway Reservoirs, along with several other V&P accessories (VP 540DB, VP 550A, VP 565).

     
 

The photo to the right shows a VP 549H2O being used on a CyBiWell robot.

 
 
 

The photo to the right demonstrates one of our Spillway Reservoirs on a Tecan Freedom Evo workstation.

 
 
 

The photo to the right demonstrate our Spillway Reservoirs on an Apricot TPS-384 robotic instrument.

 
 
 

Another feature of the VP 549ETOH-4 and VP 549H2O-4 Reservoirs is flexibility with regard to configuration of the inlet and outlet ports. Both Reservoirs come with threaded holes for two inlet/outlet pairs. The first pair is along one long side (see photo of Reservoir on CyBiWell on right).

 
 
 

The second inlet/outlet pair are on the two opposing short ends (see photo on right). A set of screw-in plugs is provided with each Reservoir so the configuration that is best suited for the particular application can be used. Two polypropylene elbow fittings are also included for flexibility in positioning the inlet and outlet hoses.

 
 
 

The VP 549ETOH-4 and VP 549H2O-4 Reservoirs have 2 guide pins along one long side. These pins allow placement of our Blotting Media Supports (either VP 540A for 96 wells, or VP 540B2 for 384 wells) plus the accompanying Lint-Free Blotting Media (either VP 540AC for 96 wells, or VP 540BC-1 for 384 wells) over the reservoirs. This feature can greatly facilitate washing and blotting of Pin Tools, particularly when robotic deck positions are limited.

 
 
 

The photos on the right show the two pins located along one long side to allow the placement of the Blotting Media Support and Blotting Media on the top of the Reservoir.

 
 
 

The photos on the right also illustrate how the Blotting Media Support and Blotting Media register and stack together on top of the Reservoir, thus occupying only one position on the deck. For applications on robotic decks with limited deck positions, these features are particularly useful.

 
 
 

The photos on the right illustrate how this system works on a CybiWell robot. The pins are lowered through the holes in the Blotting Media and Blotting Media Support into the liquid in the Reservoir. The pins are then raised, moved laterally to one side, and then lowered onto the lint- free Blotting Media to blot the excess liquid from the pins in an efficient wash process.

 
 
 

One customer found that our VP 549 series Reservoirs saved three deck positions, allowing him to carry out a significantly optimized assay. IMPORTANT NOTE: Blotting of aqueous solutions on top of the Reservoir with this system in the 96 or 384 format may not be optimal due to rapid saturation of the blotting media. Always monitor the blotting efficiency, particularly in the 384 well format.

 
 
 

Liquid levels in the Spillway Reservoirs can be easily and inexpensively maintained by use of the V&P Reagent Dispenser. In this system, a reagent bottle is connected to each of the Spillway Reservoirs (VP 549ETOH-4 or VP 549H2O-4) using tubing with a flow restrictor in the line. Each system also includes a pinch valve to help regulate the flow of liquid down the tubing to the Reservoir. The V&P Reagent Dispenser can be used with any of the wash solutions during Pin Tool operations.


See a video of one of our customers at UC Riverside using this "infinite" Reservoir system in his High Throughput Screening application.

 
 
 

The plate carriers or plate nests on the decks of some robotic platforms interfere with the inlet and outlet ports on the VP 549ETOH-4 and VP 549H2O-4. This problem has been solved by placing 4 alignment holes on the bottoms of the Reservoirs. The alignment holes (arrows) register to 4 set screws in our VP 581A and VP 581B Block Adaptors. By positioning the Reservoir on top of the SBS Block Adaptor, the Reservoir is elevated above any obstruction.

 
 
 

The upper photo to the right shows the bottom view of a Spillway Reservoir and the top view of a VP 581A SBS Block Adaptor. The lower photo on the right shows the Reservoir positioned on top of the VP 581A SBS Block Adaptor.

 
 
 

Another option for positioning the VP 549ETOH-4 and VP 549H2O-4 Spillway Reservoirs on robotic decks is the use of the VP 581R, a polypropylene Block Adaptor with an SBS pocket on top. The photo to the right shows the earlier version VP 549ETOH positioned on top of the VP 581R Block Adaptor on a robotic deck.

 
 
         

   Previous Versions of the Spillway Reservoirs

 
 

The first pair of VP 549ETOH and VP 549H2O Reservoirs had part numbers without version numbers. As more features were added as described above, the Spillway Reservoirs were given version numbers (for example, VP 549ETOH-2 and VP 549ETOH-3). The current versions, VP 549ETOH-4 and VP 549H2O-4, include all features described above.

     
         

(New)
   Rapid Flush Spillway Reservoirs

 
 


VP 549ETOH-RF & VP 549H2O-RF

     
 


Both the VP 549H2O and VP 549ETOH spillway reservoirs can be converted into rapid flush reservoirs by adding an additional large ID outlet nipple to the reservoir and using a large ID nipple for the inlet.  This design facilitates the rapid exchange of wash fluids.  This rapid exchange is important  for applications requiring fresh wash solutions between each cleaning.  By using the spill way design, the fluid level in the reservoir is automatically determined thus eliminating the need for an expensive liquid level determining device.

This rapid flush method can also be used to reduce the number of wash stations on the robot deck. 

VP 549ETOH-RF

Specifications:

Dimensions - 127.76 mm long by 85.48 mm wide, 28 mm tall
Reservoir depth = 23.8 mm
Reservoir volume = 160 ml

VP 549H2O-RF

Specifications:

Dimensions - 127.76 mm long by 85.48 mm wide, 28 mm tall
Reservoir depth = 23.8 mm
Reservoir volume = 160 ml

 
 
 
VP 549PE-1
     
 


The VP 549PE-1 Spillway Flowing Reservoir Wash System offers several advantages.  First the wash liquid is injected into the reservoir through 96 inlet ports in the bottom of the reservoir and the liquid fills the reservoir to a height of 30 mm before it runs over the main notch (there are 4 other emergency notches) in the spillway and the liquid is collected in a separate peripheral reservoir connected to the drain.  This keeps the wash reservoir uniformly flushed with new liquid coming in the bottom and waste liquid flowing out the top.  It maintains the height of the liquid at a constant level regardless of the flow rate.  The spillway feature is also important in washing 1536 pin tools because the significant displacement of wash liquid by 1536 pins can raise the level of liquid in a fixed reservoir.

The reservoir inlet and outlet quick disconnects are located on the  bottom of the reservoir.  This unit was designed for the PerkinElmer MiniTrak where the deck is open and inlet and outlet hoses can be brought in from the bottom.  The bottom of the reservoir also has locating holes which allow the reservoir to be seated securely on the deck.

 

 
 


VP 549PE-EP3

     
 


The VP 549PE-EP3 Spillway Flowing Reservoir Wash System offers the same advantages of the VP 549PE. First the wash liquid is injected into the reservoir through 96 inlet ports in the bottom of the reservoir and the liquid fills the reservoir to a height of 30 mm before it runs over the main notch (their are 4 other emergency notches) in the spillway and the liquid is collected in a separate peripheral reservoir connected to the drain.  This keeps the wash reservoir uniformly flushed with new liquid coming in the bottom and waste liquid flowing out the top.  It maintains the height of the liquid at a constant level regardless of the flow rate.  The spillway feature is also important in washing 1536 pin tools because the significant displacement of wash liquid by 1536 pins can raise the level of liquid in a fixed reservoir. 

The bottom view of the reservoir illustrated in the photo on the right reveals that the difference between the VP 549PE and the VP 549PE-EP3 is the location of the inlet and outlet quick disconnects on the  side of the reservoir.  This unit was designed for the PerkinElmer EP3 where the deck is closed and inlet and and outlet hoses can be brought in from the side.  The bottom of the reservoir also has alignment holes and locating pins which allow the reservoir to be seated securely on the deck.

 

 

 
  Basic Blotting System

     
 


If cycle speed and number of robot station positions are not important in your system, we also offer a solid lint free blotting media (VP 540D) that  has notched corners so it fits into an Omni Tray (VP 413).   The Omni Tray has a foot print of 127.7 mm long by 85.5 mm wide (SBS standard) and the VP 540D solid blotting media measures 114 mm long and 74 mm wide. Just place the solid blotting media into an Omni tray and use in conjunction with 2 VP 540 single wash reservoirs to clean the pin tool.  Ideal for blotting alcohol rinses.  The VP 540D comes 10/package, and the VP 540D-100 comes 100/package for bulk discount purchases.
 

 
 
 

VP 540D

 
 
  Lint free blotting media, cut to fit into an omni tray, 10/pkg    
 

VP 540D-100

   
  Lint free blotting media, cut to fit into an omni tray, 100/pkg    

 
  Heavy Duty Blotting System

     
 


The heart of our Heavy Duty Blotting System (VP 540DB) is a super absorbent polypropylene pad that will hold up to 27ml of fluid before it is saturated.  The pad comes in an Omni Tray with a single VP 540 Lint-Free paper on top.  The blotted liquid is transferred from the Lint-Free paper to the pad and a barrier holds it in the pad.   This super absorbent pad means you don't have to change blotters frequently.  Runs of over 1000 blots or plates are possible even with 1536 pin tools.    The VP 540DB comes 10/package, and the VP 540DB-100 comes in 10 packages of 10/package for bulk discount purchases. The super absorbent polypropylene pad (VP 540DB1) can also be purchased separately and used with plate lids and the lint free paper to save money. The VP 540DB1 comes 10/package, and the VP 540DB1-100 comes 100/package for bulk discount purchases.

The Omni Tray with its standard microplate foot print can be accommodated on any robot deck.

The photo to the right shows the blotting system on an Apricot Designs TPS-384 robot.

Here's a video of a Biomek Robot using one of our pin tools and some accessories (VP 540DB, VP 550A, VP 549ETOH, VP 565).

 

 
 


VP 540DB1

 
 
 

Super absorbent polypropylene pad cut to fit in an omni tray, 10/pkg

 
 


VP 540DB1-100

 
 

Super absorbent polypropylene pad cut to fit in an omni tray, 100/pkg

 
 


VP 540DB

     
 


Heavy duty blotting system, super absorbent pad with lint-free blotting media in an omni tray, 10/pkg

 
 
 


VP 540DB.4

   
 


Heavy duty blotting system, super absorbent pad with lint-free blotting media in an omni tray, 4/pkg

   
 


VP 540DB.6

   
 


Heavy duty blotting system, super absorbent pad with lint-free blotting media in an omni tray, 6/pkg

   
 


VP 540DB-100

     
 

Heavy duty blotting system, super absorbent pad with lint-free blotting media in an omni tray, 10 packages of 10

 
 
         

  Pin Tool Dryer

     
 

VP 904

     
 

The Pin Tool Dryer can be located on most robot decks using the SBS locating feature (VP 581B) on the bottom of the Dryer. The arrow in the photo to the right demonstrates the activation switch.  When the bottom float plate of the Pin Tool depresses the switch, the fan is turned on and dries the pins.  When the Pin Tool is removed the fan is turned off.  It doesn't get much simpler than this.

     
         


  Universal Applications

     
 

The combination wash and blotting systems can be used on any robot system and also with variety of different pin tools (E-clip type, Standard tube type, Tomtec, PerkinElmer and CyBio tube type) and even as a method to clean pipet tips.  The diameter of pin and or pipet tip however may limit their application to single chamber/fountain wash and blot systems.

 


  Efficiency of Washing and Blotting FP3 Pins Through 25 Transfers

 
 

An experiment was conducted to determine the efficacy of one water wash followed by blotting then one isopropanol wash followed by blotting. Each FP3 pin carries about 73 nl of DMSO which contained 172,832 units of FITC (0.0000106 mg FITC).  The results clearly indicate that even after 24 separate transfers the double wash and blot procedure keeps the pins clean.

The level of FITC units in both the first and twenty fourth plate are background levels.  These results clearly indicate that two wash and two blot steps are sufficient to clean the pins between source plates and that the wash solution does not need to be changed for at least 24 transfers as no significant increase in FITC units was detected even after 24 transfer and wash steps.  Because there was no detectable difference between the first plate and the twenty fourth it can be argued that many more transfers could safely be performed without changing the wash reservoirs.

 


  Wash and Blot System for Robot Pin Tools

     
 
Replacement wash reservoir for VP 540, 3.2 cm tall, with 10 and 7 mm thick walls for greater structural integrity and with slots for 11 VP 540F-1 baffles VP 540-1

Blotting Media Support for VP 540-1 with 96 holes

VP 540A
96 hole Lint-Free Blotting Media, 10/pkg VP 540AC
Blotting Media support for VP 540-1 with 384 holes VP 540B1
384 hole Lint-Free blotting media, 10/pkg  VP 540BC
Solid Lint-Free Blotting Media to fit Omni Tray, 10/pkg VP 540D
Solid Lint Free Blotting media to fit Omni Tray, 100/pkg VP 540D-100
Heavy Duty Blotting System with Super Absorbent Pad with Lint-Free Blotting Media in an Omni Tray, 10/pkg VP 540DB
Heavy Duty Blotting System with Super Absorbent Pad with Lint-Free Blotting Media in an Omni Tray, 4/pkg VP 540DB.4
Heavy Duty Blotting System with Super Absorbent Pad with Lint-Free Blotting Media in an Omni Tray, 6/pkg VP 540DB.6
Heavy Duty Blotting System with Super Absorbent Pad with Lint-Free Blotting Media in an Omni Tray, 10 packages of 10/pkg VP 540DB-100
Super Absorbent Pad cut to fit in an Omni Tray, 10/pkg VP 540DB1
Super Absorbent Pad cut to fit in an Omni Tray, 100/pkg VP 540DB1-100
Baffle system for VP 540PCB wash reservoir, 13/pkg VP 540F
Baffle system for VP 540-1 wash reservoir, 11/ pkg VP 540F-1
Autoclaveable polypropylene reagent reservoir was designed for applications on carousel robotic decks. VP 540L
Baffle system for VP 540L wash reservoir, 7/ pkg VP 540LF
Autoclaveable PTFE reagent reservoir was designed for applications on carousel robotic decks VP 540L-PTFE
Baffle system for VP 540LF-PTFE wash reservoir, 7/ pkg VP 540LF-PTFE
Single wash reservoir made from PTFE, Cybi Well style,  with slots for 13 baffles VP 540PCB
Single reservoir model, SBS foot print 3.2 cm tall with a fill and drain nipple,  with slots for 13 baffles   VP 540T
Dual interdigitated wash reservoir model, SBS foot print 3.2 cm tall  VP 541
Dual interdigitated wash reservoir model, SBS foot print 3.2 cm tall  with a fill and drain nipple VP 541T
Single wash reservoir model, SBS foot print 1.5 cm tall,  with slots for 13 baffles VP 542
Baffle system for VP 542 wash reservoir, 13/pkg VP 542F
Single wash reservoir model, SBS foot print 1.5 cm tall with a fill and drain nipple,  with slots for 13 baffles VP 542T
96 wash fountain model (CyBio foot print but can also be used in other systems) with nipples   VP 545
384 wash fountain model (CyBio foot print but can also be used in other systems) with nipples VP 546
384 wash fountain model (Universal foot print) with luer fittings VP 546A
384 wash fountain model (Caliper (Zymark) but can also be used in other systems) with nipples VP 546A1T
384 wash fountain model (CyBio foot print) with luer fittings VP 546B
384 wash fountain aluminum reservoir with restricted inlet holes. hydrophilic coating VP 546C
384 wash fountain aluminum reservoir with restricted inlet holes. hydrophilic coating - 2.87 mm diameter openings VP 546C-1
96 dual wash fountain model (CyBio foot print but can also be used in other systems)  VP 547
Spillway flowing reservoir wash system, SBS standard foot print, for ethanol, isopropanol, methanol and DMSO wash solutions VP 549ETOH
Spillway flowing reservoir wash system, SBS standard foot print, for aqueous wash solutions VP 549H2O
Spillway flowing reservoir wash system VP 549PE
Spillway flowing reservoir wash system VP 549PE-EP3
Peristaltic Pump Kit includes: pump, computer control software, RS-232 cable, 25 feet of 6 mm ID Viton tubing, 25 feet of 8 mm ID Viton tubing, 3 pump heads, mounting for 3 heads VP 560
Omni Tray carriers for VP 540D lint free media, 60/pkg VP 413
V&P Pin Cleaning Solution VP 110
 


  Pin Tool Wash, Blot and Other Accessories Kits

     
 

For your convenience we have grouped pin tool accessories into several kits that are specific for different robot systems or applications. We highly recommend that you order one of these Pin Tool Accessory kits at the time you order the pin tool.  The "essentials" these kits are the wash reservoirs, the VP 540DB Lint Free Blotting System used to clean the pins efficiently between samples, the VP 110 Pin Cleaning Solution is used the clean the pins daily, the VP 425 Pin Cleaning Brush is used if a build up occurs and the VP 555 Safe Pin Tool Storage System used to keep it clean and the fragile pins out of harms way.   Although we have several other wash and blot accessories available these are the most cost effective for the specified robot system or applications.  If you elect to use the fountain and flowing reservoirs we still strongly recommend the other items in these kits.  When purchased as a Kit you will receive a 5% discount over the sum of the individual items.

 
 
 

Robot Dual Combination Wash and Blot Accessory Kit for 96 Pin Tools - VP OK 15

Pin Wash Reservoirs (2) VP 540
96 Hole Blotting Media Support (1) VP 540A
96 Hole Lint Free Blotting Media (1 pkg) VP 540AC
Pin Cleaning Solution (1) VP 110
Pin Cleaning Brush (1) VP 425
Safe Pin Tool Storage Station (1) VP 555
 
 
 

Robot Dual Combination Wash and Blot Accessory Kit for 96 Pin Tools - VP OK 15

Pin Wash Reservoirs (2) VP 540
96 Hole Blotting Media Support (1) VP 540A
96 Hole Lint Free Blotting Media (1 pkg) VP 540AC
Pin Cleaning Solution (1) VP 110
Pin Cleaning Brush (1) VP 425
Safe Pin Tool Storage Station (1) VP 555
 
   
 

Robot Dual Combination Wash and Blot Accessory Kit for 384 Pin Tools - VP OK 16

Pin Wash Reservoirs (2) VP 540
384 Hole Blotting Media Support (1) VP 540B1
384 Hole Lint Free Blotting Media (1 pkg) VP 540BC
Pin Cleaning Solution (1) VP 110
Pin Cleaning Brush (1) VP 425
Safe Pin Tool Storage Station (1) VP 555
 
   
 

Standard Robot Pin Tool Accessory Kit - VP OK 17

Lint Free Blotting System with pad (1pkg) VP 540DB
Pin Wash Reservoirs (2) VP 540
Pin Cleaning Solution (1) VP 110
Pin Cleaning Brush (1) VP 425
Safe Pin Tool Storage Station (1) VP 555
 
   
 

Robot "Hot Swapable" Pin Tool Accessory Kit - VP OK18

Pin Tool Docking Station for "hot swapping" (1) VP 550
Lint Free Blotting System with pad (1 pkg) VP 540DB
Pin Wash Reservoirs (2) VP 540
Pin Cleaning Solution (1) VP 110
Pin Cleaning Brush (1) VP 425
Safe Pin Tool Storage Station (1) VP 555
 
   
 

Low Profile Pin Tool Accessory Kit - VP OK19

Low Profile Pin Wash Reservoirs (2) VP 542
Anti-Splash Baffles for VP 542 (2 sets) VP 542F
Lint Free Blotting System with pad (1 pkg) VP 540DB
Pin Cleaning Solution (1) VP 110
Pin Cleaning Brush (1) VP 425
Safe Pin Tool Storage Station (1) VP 555
 
 
Click here for a list of published articles that cite using the products on this page.
 
         

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