Slot Pin Replicators

     


  96 Slot Pin Multi-Blot™ Replicator

     
 


Slot Pin Replicators provide an easy quantitative way to transfer liquids from microplates to membranes, MADGE gel wells, agar or to another microplate. The slots fill automatically by capillary action and then release the liquid to a surface or another liquid.  They can be used to make arrays when used with our registration accessories: COLONY COPIERS VP 380, VP 380D, LIBRARY COPIER™ VP 381, MULTI-PRINTS VP 382, VP 382B and VP 385.  The typical arrayed material is of cDNA, DNA, RNA, oligos, PCR products, PCR digests, BAC libraries, YAC libraries, plasmid libraries, cosmid libraries, phage display libraries, antibodies, antigens and antibiotics.

 
 
 


By simply choosing the slot we can change the volume of liquid transported by Replicators and therefore the size of the spot.  Our standard Slot Pin Replicators have stainless steel 1.58 mm diameter pins, each with a slot precisely cut in the tip.  Each pin transfers a precise amount of liquid because the size of the slot directly determines the volume of liquid transported.  We have standard slots of 0.5, 1, 2, 5,10 and 25 ul and we provide them in 2 basic lengths of 22 mm and 45 mm.  The 22 mm are for standard microplates and the 45 mm long slot pins are for deep well microplates.

 

Click here for a document showing Macro-arrays prepared using V&P Scientific’s MultiBlot Repicators and MultiPrint registration devices.


Click here for a list of published articles that cite using the products on this page.

 
 


  10 and 25 ul Slot Pin Multi-Blot™ Replicators - 96 pins

     
 
These replicators were made in response to scientists that want to deliver larger inoculums (usually yeast) to an agar Omni Tray or a membrane.  To make slots that will yield the larger volumes we have to use pins of a larger diameter.   The end view of the slot is illustrated in photo to the right.  The 25 ul P11AS25 pins are 4.75 mm in diameter and the 10 ul P13S10 pins are 3.17 mm in diameter.

We can also make custom slot pins that will deliver as little as 6 nl to meet your specific needs.

Also see our 8, 12, 16 and 24 Replicator Strips with slot pins and 8, 12, 16 and 24 Floating Replicator Strips with slot pins. These are good for loading gels and making microarrays on glass slides.

The 22 mm long pins are better for loading MADGE Gels or inoculating onto an agar surface.  Both of the pins are used to make quantitative liquid to liquid transfers or liquid to membrane blots. 

 

 
 



VP 408S10

     
 


The VP 408S10 Multi-Blot Replicator in the photo to the right uses the P12S10 pin.

Please note that although the slots in the pin are a precise volume, the liquid that is transferred is usually more.  The reason for this is due to the surface tension of the liquid causing the liquid in the slot to "bow out" thus increasing the volume of the liquid in the slot.  If it is important for you to transfer exactly a certain volume, we can make custom slot pins to match the surface tension characteristics of your liquid.

Please visit this hyperlink for pin tool data and charts    

Please visit this hyperlink for customer pin tool data

Please visit this hyperlink for a technical note on how to clean the slot pins

 
 


  96 Slot Pin Multi-Blot Replicators

     
 


22 mm Long Slot Pins

     
  The nylon membrane on the right was blotted with a 5 ul Slot Pin Replicator (VP 408S5) from a microplate with serial 4 fold dilutions done in quadruplicate from blue and green dye.  Note that the size of the blot gets smaller with greater dilution.  This is because the dye actively binds to the membrane. The size of the "wet blot" is the same in all the blots even though the color blots decrease in diameter with dilution.  This phenomena is due to the active binding of the dye to membrane as it wicks away from the slot pin. Thus the bound material is concentrated at the contact point between the slot pin and the membrane and capture spot size grows only when the binding capacity of the membrane is exceeded.

Consequently, scientists can take advantage of this technique to concentrate small quantities of DNA or other binding substances onto a membrane. These membrane blots were photographed and scanned into a jpg file and the image processed on ImaGene software to capture and express the total ( integral ) blot pixel intensity. 

The total pixel intensity data are reported as the background subtracted mean +/- the Coefficient of Variation.  The ImaGenesoftware is available through BioDiscovery, Inc. in Los Angeles, (310) 207-4463

Click here for a list of published articles that cite the products on this page.

   
 
96, ~0.5 ul Slot Pin MULTI-BLOT™ Replicator, pin length =22 mm, pin diameter=1.58 mm VP 408S.5
96, ~1 ul Slot Pin MULTI-BLOT™ Replicator, pin length = 22 mm, pin diameter = 1.58 mm VP 408S
96, ~2 ul Slot Pin MULTI-BLOT™ Replicator, pin length = 22 mm, pin diameter = 1.58 mm VP 408S2
96, ~5 ul Slot Pin MULTI-BLOT™ Replicator, pin length = 22 mm, pin diameter = 1.58 mm VP 408S5
96, ~10 ul Slot Pin MULTI-BLOT™ Replicator, pin length = 22 mm, pin diameter = 3.15 mm VP 408S10
 


  Slot Size and Slot Volume

     
 


The size of spots on nylon or other charged membranes depends on the concentration of dye, DNA or other charged material in the blotted specimen. As the concentration is diluted the charged membrane captures and contains it closer to the slot pin contact point, although the liquid solvent wicks from the slot pin and makes as large a spot as the undiluted concentration of dye seen in the photo on the left.  This solvent ring is not visible in the photo.  The concentration effect of the charged membrane and the solvent wicking action can be used to increase assay sensitivity.  Thus, high density arrays can be made where the solvent spots overlap each other but the DNA or dye spots do not.  

The spot size diameter of aqueous solutions on a Nytran Super Charged Nylon Membrane are as follows: 

0.5 ul = 1.8 mm, 1.0 ul = 2.2 mm, 2.0 ul = 3 mm and 5 ul = 4.25 mm 

 
 


  96 Slot Pin Multi-Blot Replicators

     
 


45 mm Long Fixed Slot Pins

     
 
96, ~0.5 ul Slot Pin MULTI-BLOT™ Replicator, pin length = 45mm, pin diameter=1.58mm VP 408S.5a
96, ~1 ul Slot Pin MULTI-BLOT™ Replicator, pin length = 45mm, pin diameter = 1.58mm VP 408Sa
96, ~2 ul Slot Pin MULTI-BLOT™ Replicator, pin length = 45mm, pin diameter = 1.58mm VP 408S2a
96, ~5 ul Slot Pin MULTI-BLOT™ Replicator, pin length = 45mm, pin diameter = 1.58mm VP 408S5a
 

 


  384 Slot Pin Multi-Blot™ Replicator

     
 


22 mm Long Slot Pins

 

 
 

The 1 ul Slot Pins make a 2.75 mm diameter blot with 1.75 mm space between blots for distinct resolution. 

   
 
384, ~.5 ul Slot Pin MULTI-BLOT™ Replicator, pin length=22mm, pin diameter=1.58mm VP 384S.5
384, ~1 ul Slot Pin MULTI-BLOT™ Replicator, pin length=22mm, pin diameter=1.58mm VP 384S
384, ~2 ul Slot Pin MULTI-BLOT™ Replicator, pin length=22mm, pin diameter=1.58mm VP 384S2

   


  384 Slot Pin Multi-Blot™ Replicator

     
 


45 mm Long Slot Pins

 

 
 

These Replicators with their 45 mm long pins will reach the bottom of all the 384 deep well blocks on the market including the Whatman Uniplate 400. 

 

We highly recommend that you use the VP 381A Library Copier to assist in placing the replicator pins into the proper wells.  Follow this link for more information.

   
 
384,~0.5 ul Slot Pin MULTI-BLOT™ Replicator, pin length=45mm, pin diameter=1.58mm VP 384S.5a
384, ~1 ul Slot Pin MULTI-BLOT™ Replicator, pin length=45mm, pin diameter =1.58mm VP 384Sa
384, ~2 ul Slot Pin MULTI-BLOT™ Replicator, pin length=45mm, pin diameter =1.58mm VP 384S2a
   


  Membrane Macroarray Starter Kit Accessories

     
 


For your convenience and a 5% discount, V&P Scientific has combined the essential components of our 96 and 384 pin MULTI-BLOT™ replication system into a starter kit to make up to 4 identical 864 or 1536 feature Macroarrays at once.  You select whichever slot pin MULTI-BLOT™ Replicator fits your application and delivery volume,  then just add it to the Basic Macroarray Starter Kit VP OK14 and you have everything you need to start making arrays.  Included in the Starter Kit are the following:
 

     
 

VP OK14

Four, MULTI-PRINTS VP 382
One, LIBRARY COPIER™ VP 381 or VP 381N *
One, V&P Pin Cleaning Solution Bottle VP 110
One, Package Of Lint Free Blotting Paper VP 522
One, Pyrex Alcohol Reservoir With Lid VP 420
One, Clear Overlay Reading Mat For 864 And 1536 Arrays VP 430
Five, Plastic Bleach Or Water Reservoirs VP 421
One, Pin Cleaning Brush VP 425
One, 30 cm By 3 Meter Roll Of Hybond N Nylon Membrane VP 503
* Use the VP 381N for Floating Slot Pin Replicators
 


  Nanoliter Slot Pins

     
 


Our smallest standard slot size is 6 nl.  These are used primarily in arraying on glass slides.  For liquid to liquid delivery with slot pins we recommend using floating pin tools.  With the floating pin tools we have slot  volumes that range from 10 nl to 50 nl. 

 
 


  Slot Pin Replicators To Load Madge Gel Wells

     
 


V&P Scientific, Inc. has developed a MADGE Gel Registration Blockthat provides a simple register of our 96 MULTI-BLOT™ Slot Pin Replicators to the wells in the MADGE Gel.  This greatly simplifies loading the MADGE Gel wells with PCR product.

The Registration Block visually aligns with the 96 wells in the MADGE Gel.  The two guide pins on the MULTI-BLOT™ Replicator fit into two alignment holes on the Registration Block and insure that the Replicator slot pins are aligned above the wells for accurate delivery. 

All of the V&P Scientific Slot Pin MULTI-BLOT™ Replicators have guide pins that are compatible with the alignment holes on the Registration Block, so any volume from 0.1 ul to 10 ul can be delivered to the wells.  However, most investigators use either the 2 ul or 5 ul Slot Pin MULTI-BLOT™ Replicators (VP 408S2 or VP 408S5)

 
 


  Custom Slot Pin Multi-Blot™ Replicators

     
 


Make a custom Slot Pin Replicator to fit your application.  Just select slot volume, pin length, pin diameter and pin format (48, 96, 384, 1536). 

The enlarged 0.787 mm diameter floating slot pins in the photo on the right illustrates our flexibility in making slots of different widths (0.25 mm to 0.45 mm) and depths.  The 0.787 mm diameter pins have the advantage of a very small foot print and yet can be slotted to carry a large volume of specimen.  For comparison purposes we have included a standard paper clip in the photo. 

 
 


  Custom Light Weight (Swiss Cheese Style) Slot Pin Replicators

 
 
One of our customers requested an ultra light weight Multi-Blot Replicator for a particular application so we designed a Replicator base with all of the excess aluminum removed.  We call it the "Swiss Cheese Style".  If you need a light Replicator just request a custom "Swiss Cheese."  If you use your replicators constantly throughout the day as many labs do.  You will come to appreciate a light weight replicator.  We can also make them with  384 pins and with slot pins.
 
 


  Floating Tube Type Slot Pin Multi-Blot™ Replicators

     
 
The advantage of floating slot pins is that the stainless steel pins have a precision slot cut into the tip of the pin to precisely pick up liquid via capillary action.  Each pin slides into a hole in a floating assembly until it "floats" on the gridding surface and delivers via capillary action, liquid from the slot at the tip.  This floating action assures that each of the pins will deliver its liquid even if the blotting/gridding surface is not level or smooth.  Manual Multi-Blot Floating Slot Pin Replicators and Robot Floating Slot Pin Replicators are available in 96, 384, and 1536 formats.  The standard slot volumes for tube style pins available range from 6 nl to 500 nl.  A complete description of the floating slot pin replicators and how to order them is found on the floating pin page.
 

 


  Floating E-Clip Type Slot Pin Multi-Blot™ Replicators

     
 
The advantage of the E-Clip floating slot pins is that they carry a larger volume than the Tube style slot pins. Each  precisely picks up liquid via capillary action and delivers it on contact with a liquid or solid surface.  Each pin slides in a floating assembly until it "floats" on the gridding surface and delivers via capillary action, liquid from the slot at the tip.  This floating action assures that each of the pins will deliver its liquid even if the surface is not level or smooth.  Manual Multi-Blot Floating E-Clip Slot Pin Replicators and Robot Floating E-Clip Slot Pin Replicators are available in 96 and 384 formats.  The standard slot volumes for E-Clip style pins available range from 0.5 ul to 5 ul.  A complete description of the floating E-Clip slot pin replicators and how to order them is found on the floating pin page.
 

 

  Hydrophobic & Lipophobic Coating of Pins
     
 


All of the above pins can also be obtained with a hydrophobic/lipophobic coating.  Just adding an H to the above pin designates that pin as a hydrophobic/lipophobic coated pin.  This coating is metallic and only a few angstroms thick so it does not effect the basic dimensions of the pins.  These coated pins are very useful in handling solutions with proteins or lipids that may stick to naked stainless steel.  Click here for more details on the nature of our hydrophobic/lipophobic coating and the coating process.

If you are transferring DNA, cDNA or other materials in high surface tension solutions you will find that using our new V&P Pin Cleaning Solution will greatly increase the precision of delivery.  For an explanation see the FAQ page and the V&P Pin Cleaning Solution page.

Please visit this hyperlink for Pin Tool Data And Charts.

 


  Accessories

     
 


We suggest you buy a few inexpensive accessories that will make the Replicators more useful, easier, safer and more reproducible. 

The VP 381 is a Registration Device that aligns the Replicator pins to the center of the wells.  This is very important as you do not want the pins to touch the side of wells as you remove the pins because that will knock off the hanging drop at the pin tip.  The VP 381 LIBRARY COPIER™ is also used with our 96 pin Replicators to re-array from four, 96 well plates to one, 384 well plate or vice versa, to break out samples from one, 384 well plate to four, 96 well plates.

The VP 382 MULTI-PRINT™ is a registration device that allows you to make membrane arrays in an area the size of a standard microtiter plate with our Replicators.  Either four, 384 well plates or nine, 96 well plates can be arrayed into a 1536 or 864 array on the membrane.

After arraying from four, 384 well plates to one, 1536 membrane array (or nine, 96 well plates to one, 864 membrane array), the VP 430 Reading Mat helps locate which mother plate well produced a positive spot on the membrane array.

The VP 420 is a Glass Alcohol Reservoir that is used in the sterilization of the Replicators by flaming.  Some labs use plastic reservoirs, but if flaming alcohol drops into them the plastic burns, dumping flaming alcohol all over the counter.  This covered dish is also useful for storing the alcohol as well as VP 110 Pin Cleaning Solution (below) for reuse.  

The VP 425 is a Pin Cleaning Brush that is used to keep the Replicator pins clean so they will carry the same amount of liquid each time.   

Our VP 110 is a Pin Cleaning Solution that is used to clean and treat the pin tips when they can't be flamed.  

Integral to the cleaning process of all slot pins is the blotting step between baths.  Lint particles in the slots or pin tips, from blotting onto standard paper towels, will interfere with the loading and unloading of liquid.  The VP 522 is a Lint Free Blotting Paper that doesn't transfer lint particles to the slot pins as many blotting papers or paper towels can do.  We strongly recommend that you use these blotting pads placed over several layers of paper towels. 

These accessories are also found in the Basic Membrane Array Started Kit VP OK14 at a 5% discount.

 


  Cleaning Pins

     
 


The most important aspect of using pin tools is to start with clean pins and then keep them clean so they will consistently deliver the same volume.  We recommend our Pin Cleaning Solution (VP 110) to clean the pin of protein and other residue buildup before you start an assay.  To quickly and efficiently remove the previous transfer from the pin we have a wide variety of manual and robot wash reservoirs ranging from disposable polypropylene reservoirs (VP 576D) to reusable wash reservoirs, flowing reservoirs and fountains to clean  96, 384 and 1536 pin tools.  The robot wash stations are also adapted so the blotting station is part of the washing station thus reducing the cleaning cycle time and the number of robot stations required for the cleaning process.  We also have other independent blotting stations (VP 540D) and VP 522.

Integral to the cleaning process of all pins is the blotting step between baths.  Lint particles in the slots or on pin tips, from standard paper towels, will interfere with the loading and unloading of liquid.  We have solved this problem.  The VP 540DB blotting system is a tray with the standard microplate foot print that contains a super absorbent polypropylene pad covered by a Lint Free Blotting Paper.  We strongly recommend that you use this or our other lint free blotting systems. We have prepared a detailed technical note (Technote 31) which describes the step by step process of cleaning slot pins.

 

 
 


Click here for a list of published articles that cite using the products on this page.

 
         

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