Pins for Floating Pin Replicators
     
 


Floating Pin Tools consist of three basic parts:

  1. Pins

  2. Floating Fixture Frame

  3. Mounting plate to connect to a handle for Manual Pin Tools or to a Robot mating features

Building a pin tool begins with selecting the appropriate pin for your application.  Once that decision is made, determining the Floating Frame Fixture is easy as well as the mounting plate to make a complete robot or manual pin tool.

One of the chief factors in determining the volume of liquid transferred by a pin is the pin diameter.  We offer pins that range in diameter from 0.229 mm to 4.77 mm as seen in the photo to the right.

Here's a link to an article that discusses pin tools as viable dispensing alternative.

Click here for a poster authored by V&P Scientific and ARTEL on Nanoliter Volume Pin Tool Transfers as Measured by a Dual-Dye Absorbance Method


Click here for a list of published articles that cite using the products on this page.
 

 


  Pin Length and Diameter

     
 


Tube Pins

     
 


Pins are available as 2 basic styles; the tube pins to the right are fixed inside tubes to provide greater strength to small diameter pins.  The pins vary in diameter from 0.229 mm to 0.914 mm, and they also vary in the total length of the pin (from 33 to 50 mm) and the length of the exposed pin from 12 to 30 mm long.  The exposed length terminology is used to define how far the pin is able to extend below the bottom float plate thus it subtracts the thickness of the lower float plate from the length of the pin that protrudes from the tube. The 12 mm long exposed length pins are used on robots with "Z" limitations and the 30 mm long exposed length pins are used when delivering to or from the bottom of a deep well plate.  For all other applications either the standard (FP_ series) 17 mm long exposed length or the shorter overall length (38 mm FP_N series) with a 17 mm long exposed length pins.  Which one you select is based on the "Z" limitation of your robotic systemThe diameter of the mounting tubes for all these pins is 1.58 mm.

 
 
 


E-Clip pins

     
 


The E-Clip pins shown the photo to the right are fixtured with an E-Clip in a groove in one end of the pin.  These pins are either 1.58 mm, 2.36 mm, 3.18 mm or 4.77 mm in diameter.  The larger diameter pins carry more liquid and are preferred by scientists inoculating yeast on to agar plates.  We offer pins of longer lengths for scientists using deep well blocks.  The FPFS2 pins have the E-Clip groove part way down the shaft to allow it to be used as a spring loaded floating pin useful in sampling frozen specimens.

We offer six styles of E-clip Pins: the FP pin which has a chamfered tip leading to a point, the FP6 which has the point cut off and delivers a larger drop of inoculum.  The FP pin delivers a smaller amount of liquid due to the point on the end of the pin.  It is well suited for making high density arrays on membranes and agar surfaces.  The flat tip FP6 pins are used to transfer larger volumes of liquid and the FP11A, FP12, FP12A and FP13 pins which transfer the largest volumes are used by customers transferring yeast to agar plates.

All the E-Clip pins (FP, FP6, FPAM, FPFS2, FP11A , FP12, FP12A and FP13 pin can be used with the Robot style double floating frames, or with double float frames that have been converted to manual Multi-Blot replicators with our conversion kits  (BGPK or BGPKL).

The following sections will help guide you through the selection process.  If you have  questions please feel free to contact us at (800) 455-0644.

 

 
 


***NEW***
Pins for the 3456 Mcroplates

     
 


One of our customers wanted to know if we can make a pin tool for the new 3456 microwell plates.  Yes, we can - see the photo at the right.  By simply using a smaller cannula we can make a pin tool with only a 0.75 mm center to center pitch.  We can easily accommodate both our FP1 (0.457 mm) and FP9 (0.229 mm) diameter pins.

 
 


  Pin Designations

     
 


The FP (Floating Pin) and a number refer to the pin diameter, the C means it is a shorter pin, T is  a taller or longer exposed length pin.  Some robots have height or "Z" limitations and need to have the shortest overall height possible.  Pins for deep well plates need to be longer to reach the bottom of the well.  The H means the pin has a hydrophobic coating,  S means the pin has a slot and the number after the S indicates the nanoliter or microliter volume of the slot.

     
   

Pin Diameter

FP4 = 0.914 mm (tube) NEW
FP3 = 0.787 mm (tube)
FP1 = 0.457 mm (tube)
FP8 = 0.356 mm (tube) NEW
FP9 = 0.229 mm (tube)
FP = 1.58 mm (E-Clip) chamfered tip
FP6 = 1.58 mm (E-Clip) NEW flat tip
FPAM = 1.58 mm (E-Clip) NEW chamfered tip for Deep well microplates
FPFS2 = 1.58 mm (E-Clip) NEW 2 ul slot tip
FP11A = 4.75 mm  (E-Clip) NEW diameter flat tip
FP13 = 3.18 mm  (E-Clip) NEW diameter flat tip
FP12 = 2.36 mm (E-Clip) NEW diameter flat tip
FP12A = 2.36 mm (E-Clip) NEW diameter flat tip
 
 
Slot Pin = S
number after the S = nanoliter or ul volume of slot
Hydrophobic/lipophobic coating = H
 
     
 
 

Pin length

T = tall pin (50.8mm long - exposed length =  30 mm long) NEW
No C = standard pin (50.8 mm long - exposed length = 17 mm long)
C = short tube and pin (33 mm long total- exposed length = 12 mm long)
N = short tube and long pin (38 mm long  - exposed length = 17 mm long) NEW
Robot systems that often require the shorter pins (CB and N series) because of clearance issues are the CyBio, MULTIMEK, MULTIPETTE, PlateMate and Perkin Elmer systems
34 mm long, exposed length = 23 mm
33 mm long, exposed length = 22 mm
63.5 mm long, exposed length = 45 mm
50 mm long, exposed length = 26 mm
27 mm long, exposed length = 22 mm
51 mm long, exposed length = 39 mm
 
Solid pin = no S
No coating = no H
     


The tables below contain hyperlinks to the various types/lengths/diameters/coatings of the pins we offer.

 
 

Tube Style Pins

     
   

Uncoated

12 mm/33 mm length - solid pins 17 mm/38 mm length solid pins 17 mm/50 mm length solid pins 30 mm/50 mm length solid pins
12 mm/33 mm length slot pins 17 mm/38 mm length slot pins 17 mm/50 mm length slot pins 30 mm /50 mm length slot pins
 
   

Hydrophobic coated

12 mm/33 mm length solid pins 17 mm/38 mm length solid pins 17 mm/50 mm length solid pins 30 mm/50 mm length solid pins
12 mm/33 mm length slot pins 17 mm/38 mm length slot pins 17 mm/50 mm length slot pins 30 mm/50 mm length slot pins
 
 


E-Clip Style Pins

     
   
Flat tip solid pins 1.58 mm diameter Pointed tip solid pins 1.58 mm diameter
Flat tip slot pins 1.58 mm diameter Pointed tip slot pins 1.58 mm diameter
Flat tip solid pins 2.36 mm diameter  
Flat tip solid pins 2.36 mm diameter for deep well microplates.  
Flat tip solid pins 3.18 mm diameter Chamfered tip solid pins 63 mm long, 1.58 mm diameter for deep well microplates
Flat tip solid pins 4.75 mm diameter
Flat tip slot pins 1.58 mm diameter - spring loaded
 


  Factors Affecting Pin Delivery Volumes

     
 


The list on below summarizes the factors that contribute to the volume delivered.  With each application these factors can be controlled and standardized so that the delivery volumes are very reproducible.  With most applications the CV's are less than 5%.

 

1. Pin Diameter
2. Surface tension of the liquid being transferred
3. Surface tension of the pin
4. Speed of removal of pin from source liquid
5. Speed of pin striking recipient dry plate
6. Depth to which the pin is submerged in source plate
7. Depth to which the pin is submerged in recipient plate
8. Volume of slot in pin
9. Surface tension of the dry plate and dwell time

10.Microplate well (24, 48, 96, 384 or 1,536)

     
 


V&P pins transfer liquid by carrying the liquid on the sides, tip and in slots we cut into the tip. The amount delivered is determined by the diameter and surface tension of the pin, the surface tension of the liquid, how far the pin is submerged into the liquid and the speed with which the pin is removed from the liquid.  When all of these variables are fixed, the accuracy of the liquid transfer is very consistent, with CV's averages better than 5%.

 
 
 


Increasing the speed of withdrawal from the source plate by 7- fold will increase the volume delivered by as much as 3 fold in a linear relationship.  This phenomenon can be exploited to expand the range of delivery volume for a single pin. The table and the chart below illustrates the effects of increasing the speed 7- fold.  The speed range is from 0.78 cm/sec to 5.7 cm/sec.  Use this chart to select the pin that will deliver in your desired range.

 
 

 
  Select The Volume You Need To Transfer For Your Application

 
 


Delivery Volume Ranges

     
   

Delivery volume range is determined by speed of withdrawal from source liquid.
slow speed  = 0.78 cm/sec = low volume delivery range
fast speed  =  5.70 cm/sec = high volume delivery range
Follow this link to assay methods

 

The links below are to delivery volume range tables for uncoated and hydrophobic coated delivering either DMSO or Aqueous solutions in liquid to liquid transfers.

DMSO TRANSFERS WITH UNCOATED AND HYDROPHOBIC COATED PINS

AQUEOUS TRANSFERS WITH HYDROPHOBIC COATED PINS

AQUEOUS TRANSFERS WITH UNCOATED (STAINLESS STEEL) PINS

     


  A Guide For Selecting The Right Pin For Your Application

     
 


1.  What volume do you need to  transfer?   DMSO? Aqueous? See these hyperlinked tables to determine your options.  Also consider custom slot pins.

2. What volume is in the source plate wells? If it is small a slot pin will have a definite advantage.

3. Will the source plate have wells with significantly different levels of liquid?  (Cherry picked source plate or edge drying effect?).  If yes and if the absolute volume transferred is critical, then select the largest slot pin that is in your transfer range.  This will minimize the effect of liquid height on the volume of liquid carried on the sides of the pin.  Also consider custom slot pins.

4. Does the material transferred bind non-specifically to stainless steel?  If yes then select the Hydrophobic/lipophobic coated pins.  If no, select the uncoated pins.

5. What is the Z clearance on the robot deck from the highest impediment (top of the source plate/recipient plate/wash reservoirs, etc.) to the top of the robot mounting plate?  If it is greater than 77 mm you can use any of pins.  If it is less than 77 mm, you can only use the shorter FP(#)C series pins (12 mm exposed pin length), the FP(#N) series pins (17 mm exposed pin length) and the E-clip series pins (23 mm exposed pin length pins.  If the Z clearance is less than 60 mm, we recommend you contact us.  There are a few tricks we can do to shave off 5 to 10 mm in height. 

Links to the specifications of all the robot mounting plates/pin tool/pin combinations can be found on the height ("Z") restriction page

6.  Is the source plate a deep well plate?  If so you may need to use a 30 mm long exposed length pin ("T" pin) to reach down to the lower levels of the well. 

7.  Should you choose the solid pin versus the slot pin? Although there appears to be a slight advantage for slot pin, CV's between the two pins are very good.  Both are easy to clean between specimens.  Biggest factor is cost.  If you don't need to deal with varying liquid heights and you can obtain the volume necessary with a solid pin, choose the economical solid pin.

8.    If you are still uncertain about which pin to select for your application, you can perform a simple "Proof of Principle" test with several different pins using our inexpensive VP 450FP3 Replicator Strip coupled via a VP 452MP to one of our robot mounting plates.   An even simpler solution is to use a work station with a 1, 4 or 8 probe dispense head with the new V&P Mono Pin Tool to test the various pins.

 


  Selecting a Pin Tool as Simple as 1, 2, 3

     
 
1. Select the appropriate pin.  For the manual and Beckman Bio Mek 2000 robots we recommend the longer FP_ series pins.

2. Select the Floating Fixture with the appropriate pattern (96, 384 or 1,536) and diameter of holes.

3. Select the robot system you wish to use the Pin Tool with.  We have made Mounting Plates to over 34 Robot Workstations.  The BMP Mounting Plate on this page accommodate the manual pin tool and the Beckman BioMek 2000 robot.

Determine the weight of a Robot pin tool at this link

 

 

 
  Convert a Robot Pin Tool to a Manual Pin Tool

     
 


Our Robotic floating Pin Tools can easily be converted to a manual pin tool with this simple BGPK  kit which contains a Guide Pin Frame and handle and sturdy mounting plate (BMPM)  The guide pins are used with our manual microplate (VP 381N) and membrane registration accessories (VP 382 and VP 382B) to keep the pins in the center of the wells and to make high density arrays on membranes or agar surfaces

Conversion of robot pin tools to manual Multi-Blot Replicators is very easy using our BGPK for Standard length pins and the BGPKL for longer pins.  Simply remove the robot mounting feature from the float plates disassemble the pin tool and reassemble it with the Guide Pin Frame as part of the tool.  Attach the Manual Mounting Plate and handle and you are ready to go.   If this sounds too daunting for you - send it back to us and we will do it for you for a nominal fee.

The main reason for this conversion is to attach  guide pins to the replicator so it can be aligned to Microplate registration accessories such as the VP 381 series Library copiers for replicating and reformatting genomic libraries, VP 380 series colony copiers to make high density arrays on agar surfaces or  VP 382 membrane printers to make high density arrays on nylon or other membranes.

The BGPKL kit is used when longer replicator pins are used like the FPAM pins which are 63 mm long.   A pin tool with FPAM pins is illustrated in the photo to the right.   In order to accommodate these longer pins we need to use a longer and more sturdy guide pin found on the BGPL frame.  These larger guide pins are 3.17 mm in diameter.

Each of the kits on the right (BGPKL and BGPK) is made up of a handle, a manual mounting plate and a guide pin frame.  The guide pin frame is the only  difference between the two kits

 

 


  Exposed Pin Length

     
 


All of our various diameter floating tube pins come in 4  exposed/overall pin lengths:
12mm exposed with overall length of 33 mm (FP_CB)
17mm exposed with overall length of 38 mm (FP_N)
17mm exposed with overall length of 50 mm (FP_) 
30 mm exposed with overall length of 50 mm (FP_T)

Depending on your application, type of robot and "Z" limitation on the robot deck, each pin  has an advantage or special feature.  Contact us if you are unsure about which pin to select.

 
 
   
Standard length solid Pins used in Double Float Plate Replicators
 
   
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.914 mm, delivery volume = ~120 nl* NEW FP4
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, delivery volume = ~80 nl FP3
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, delivery volume = ~20 nl FP1
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.356 mm, delivery volume = ~10 nl NEW FP8
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.229 mm, delivery volume = ~4 nl FP9
 
 


*The above delivery volumes for these pins are approximate values, the actual volumes delivered depend upon many controllable factors that will vary from one application to another.  For example in liquid to liquid transfers the amount transferred will include the hanging drop on the bottom of the pin as well as the liquid on the sides of the pin.  If the transfer was to a membrane then only the hanging drop would be transferred and not the liquid on the sides of the pin.  If the transfer was to a dry plate then only about 1/4 off the hanging drop would be transferred to the dry plate and the rest would be on the pin.  The division of the hanging drop would be determined by the surface tension of the pin and the surface tension of the dry plate. The speed of pin withdrawal can increase the volume transferred by 3 fold.

     
 

 
Short solid Pins used in Double Float Plate Replicators

 
   
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.914 mm, delivery volume = ~120 nl NEW FP4CB
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, delivery volume = ~80 nl FP3CB
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.457 mm, delivery volume = ~20 nl  FP1CB
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.356 mm, delivery volume = ~10 nl  NEW FP8CB
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.229 mm, delivery volume = ~4 nl FP9CB
 
       
 

***NEW***
Tall
solid Pins used in Double Float Plate Replicators

     
   
Exposed pin length =  30 mm, overall pin length = 50.8 mm, pin diameter = 0.914 mm, delivery volume = ~120 nl NEW FP4T
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, delivery volume = ~80 nl NEW FP3T
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, delivery volume = ~20 nl NEW FP1T
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.356 mm, delivery volume = ~10 nl NEW FP8T
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.229 mm, delivery volume = ~4 nl NEW FP9T
 
 


***NEW***
17 mm length pins for short tube solid Pins used in Double Float Plate Replicators

 
   
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.914 mm, delivery volume = ~120 nl* NEW FP4N
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, delivery volume = ~80 nl NEW FP3N
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, delivery volume = ~20 nl NEW FP1N
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.356 mm, delivery volume = ~10 nl NEW FP8N
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.229 mm, delivery volume = ~4 nl NEW FP9N
 
 

*The above delivery volumes for these pins are approximate values, the actual volumes delivered depend upon many controllable factors that will vary from one application to another.  For example in liquid to liquid transfers the amount transferred will include the hanging drop on the bottom of the pin as well as the liquid on the sides of the pin.  If the transfer was to a membrane then only the hanging drop would be transferred and not the liquid on the sides of the pin.  If the transfer was to a dry plate then only about 1/4 off the hanging drop would be transferred to the dry plate and the rest would be on the pin.  The division of the hanging drop would be determined by the surface tension of the pin and the surface tension of the dry plate. The speed of pin withdrawal can increase the volume transferred by 3 fold.

     


  Hydrophobic & Lipophobic Coating of Pins

     
 


All of the above pins can also be obtained with a hydrophobic/ lipophobic coating.  Just adding an H to the above pin designates that pin as a hydrophobic/ lipophobic coated pin.  These coated pins are very useful in handling solutions with proteins or lipids that may stick to naked stainless steel.

 
 
 


Hydrophobic Coated Standard length Pins used in Double Float Plate Replicators

 
   
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter= 0.914 mm, delivery volume = ~120 nl, hydrophobic coated NEW FP4H  
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter=0.787 mm, delivery volume = ~80 nl, hydrophobic coated

FP3H 

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter= 0.457 mm, delivery volume = ~20 nl, hydrophobic coated

FP1H

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter=0.356 mm, delivery volume = ~10 nl, hydrophobic coated NEW FP8H
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter=0.229 mm, delivery volume = ~4 nl, hydrophobic coated

FP9H

 
 


Hydrophobic Coated short Pins used in Double Float Plate Replicators

 
   
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.914 mm, delivery volume = ~120 nl, hydrophobic coated NEW FP4CBH
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, delivery volume = ~80 nl, hydrophobic coated

FP3CBH

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.457 mm, delivery volume = ~20 nl, hydrophobic coated

FP1CBH

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.356 mm, delivery volume = ~10 nl, hydrophobic coated NEW FP8CBH
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter=0.229 mm, delivery volume = ~4 nl, hydrophobic coated

FP9CBH

 
       
 


Hydrophobic Coated Tall Pins used in Double Float Plate Replicators

 
   
Exposed pin length =  30 mm, overall pin length = 50.8 mm, pin diameter = 0.914 mm, delivery volume = ~120 nl, hydrophobic coated NEW FP4TH
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, delivery volume = ~80 nl, hydrophobic coated NEW

FP3TH

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, delivery volume = ~20 nl, hydrophobic coated NEW

FP1TH

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.356 mm, delivery volume = ~10 nl, hydrophobic coated NEW FP8TH
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.229 mm, delivery volume = ~4 nl, hydrophobic coated NEW

FP9TH

 
 


***NEW***
Hydrophobic coated 17 mm length pins for short tube solid Pins used in Double Float Plate Replicators

 
   
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.914 mm, delivery volume = ~120 nl*, hydrophobic coated NEW FP4NH
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, delivery volume = ~80 nl, hydrophobic coated NEW FP3NH
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, delivery volume = ~20 nl, hydrophobic coated NEW FP1NH
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.356 mm, delivery volume = ~10 nl, hydrophobic coated NEW FP8NH
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.229 mm, delivery volume = ~4 nl, hydrophobic coated NEW FP9NH
 
 

*The above delivery volumes for these pins are approximate values, the actual volumes delivered depend upon many controllable factors that will vary from one application to another.  For example in liquid to liquid transfers the amount transferred will include the hanging drop on the bottom of the pin as well as the liquid on the sides of the pin.  If the transfer was to a membrane then only the hanging drop would be transferred and not the liquid on the sides of the pin.  If the transfer was to a dry plate then only about 35% of the hanging drop would be transferred to the dry plate and the rest would be on the pin.  The division of the hanging drop would be determined by the surface tension of the pin and the surface tension of the dry plate. The speed of pin withdrawal can increase the volume transferred by 3 fold.

     

 
  Slot Pins

     
 


Slot in Standard length pins used in Double Float Plate Replicators

 
   
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 6 nl

FP1S6

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 10 nl

FP1S10

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 20 nl NEW

FP1S20

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 30 nl NEW

FP1S30

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 40 nl NEW

FP1S40

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 50 nl

FP1S50

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 50 nl

FP3S50

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 100 nl

FP3S100

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 150 nl NEW

FP3S150

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 200 nl

FP3S200

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 300 nl NEW

FP3S300

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 500 nl

FP3S500

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.914 mm, slot volume = 1,000 nl FP4S1000
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.914 mm, slot volume = 2,000 nl FP4S2000
 

 
 


The FP4 slot pins in the photo on the right are used to transfer liquid to and from 1536 microplates.  The pins are small enough to fit into the wells and can be slotted to carry larger samples (1,000 and 2,000 nl).  If you want to add a larger volume to a 1536 well microplate this is the pin of choice.

 
 
 

 
Slot
in Short Pins used in Double Float Plate Replicators

 
 
 
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.457 mm, slot volume = 10 nl FP1CS10
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.457 mm, slot volume = 20 nl NEW FP1CS20
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.457 mm, slot volume = 30 nl NEW FP1CS30
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.457 mm, slot volume = 40 nl NEW FP1CS40
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.457 mm, slot volume = 50 nl FP1CS50
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, slot volume = 50 nl FP3CS50
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, slot volume = 100 nl FP3CS100
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, slot volume = 150 nl NEW FP3CS150
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, slot volume = 200 nl FP3CS200
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, slot volume = 300 nl NEW FP3CS300
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, slot volume = 500 nl FP3CS500
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.914 mm, slot volume = 1,000 nl NEW FP4CS1000
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.914 mm, slot volume = 2,000 nl NEW FP4CS2000
 
 


***NEW***
Slot in 17 mm long short pins used in Double Float Plate Replicators

 
   
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, slot volume = 6 nl NEW

FP1NS6

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, slot volume = 10 nl NEW

FP1NS10

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, slot volume = 20 nl NEW

FP1NS20

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, slot volume = 30 nl NEW

FP1NS30

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, slot volume = 40 nl NEW

FP1NS40

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, slot volume = 50 nl NEW

FP1NS50

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, slot volume = 50 nl NEW

FP3NS50

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, slot volume = 100 nl NEW

FP3NS100

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, slot volume = 150 nl NEW

FP3NS150

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, slot volume = 200 nl NEW

FP3NS200

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, slot volume = 300 nl NEW

FP3NS300

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, slot volume = 500 nl NEW

FP3NS500

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.914 mm, slot volume = 1,000 nl NEW FP4NS1000
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.914 mm, slot volume = 2,000 nl NEW FP4NS2000
 
 


***NEW***
Slot in Tall Pins used in Double Float Plate Replicators

     
   
Exposed pin length =  30 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 10 nl NEW FP1TS10
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 20 nl NEW FP1TS20
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 30 nl NEW FP1TS30
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 40 nl NEW FP1TS40
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 50 nl NEW FP1TS50
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 50 nl NEW FP3TS50
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 100 nl NEW FP3TS100
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 150 nl NEW FP3TS150
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 200 nl NEW FP3TS200
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 300 nl NEW FP3TS300
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 500 nl NEW FP3TS500
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.914 mm, slot volume = 1,000 nl NEW FP4TS1000
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.914 mm, slot volume = 2,000 nl NEW FP4TS2000
 

 
  Hydrophobic Slot Pins

     
 


Hydrophobic standard length Slot Pins used in Double Float Plate Replicators

 
   
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 10 nl, hydrophobic coated

FP1S10H

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 20 nl, hydrophobic coated NEW

FP1S20H

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 30 nl, hydrophobic coated NEW

FP1S30H

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 40 nl, hydrophobic coated NEW

FP1S40H

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 50 nl, hydrophobic coated

FP1S50H

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 50 nl, hydrophobic coated

FP3S50H

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 100 nl, hydrophobic coated

FP3S100H

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 150 nl, hydrophobic coated

FP3S150H

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 200 nl, hydrophobic coated

FP3S200H

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 300 nl, hydrophobic coated

FP3S300H

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 500 nl, hydrophobic coated

FP3S500H

Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.914 mm, slot volume = 1,000 nl, hydrophobic coated NEW FP4S1000H
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.914 mm, slot volume = 2,000 nl, hydrophobic coated NEW FP4S2000H
 
 

 
Hydrophobic Short Slot Pins used in Double Float Plate Replicators

 
   
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = . 0.457 mm, slot volume = 10 nl, hydrophobic coated

FP1CS10H

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = . 0.457 mm, slot volume = 20 nl, hydrophobic coated NEW

FP1CS20H

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = . 0.457 mm, slot volume = 30 nl, hydrophobic coated NEW

FP1CS30H

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = . 0.457 mm, slot volume = 40 nl, hydrophobic coated NEW

FP1CS40H

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.457 mm, slot volume = 50 nl, hydrophobic coated

FP1CS50H

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, slot volume = 50 nl, hydrophobic coated

FP3CS50H

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, slot volume = 100 nl, hydrophobic coated

FP3CS100H

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, slot volume = 150 nl, hydrophobic coated NEW

FP3CS150H

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, slot volume = 200 nl, hydrophobic coated

FP3CS200H

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, slot volume = 300 nl, hydrophobic coated NEW

FP3CS300H

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.787 mm, slot volume = 500 nl, hydrophobic coated

FP3CS500H

Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.914 mm, slot volume = 1,000 nl, hydrophobic coated NEW FP4CS1000H
Exposed pin length = 12 mm, overall pin length = 33 mm, pin diameter = 0.914 mm, slot volume = 2,000 nl, hydrophobic coated NEW FP4CS2000H
 
 


***NEW***
Hydrophobic slot in 17 mm long short pins used in Double Float Plate Replicators

 
   
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, slot volume = 6 nl, hydrophobic coated NEW

FP1NS6H

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, slot volume = 10 nl, hydrophobic coated NEW

FP1NS10H

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, slot volume = 20 nl, hydrophobic coated NEW

FP1NS20H

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, slot volume = 30 nl, hydrophobic coated NEW

FP1NS30H

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, slot volume = 40 nl, hydrophobic coated NEW

FP1NS40H

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.457 mm, slot volume = 50 nl, hydrophobic coated NEW

FP1NS50H

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, slot volume = 50 nl, hydrophobic coated NEW

FP3NS50H

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, slot volume = 100 nl, hydrophobic coated NEW

FP3NS100H

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, slot volume = 150 nl, hydrophobic coated NEW

FP3NS150H

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, slot volume = 200 nl, hydrophobic coated NEW

FP3NS200H

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, slot volume = 300 nl, hydrophobic coated NEW

FP3NS300H

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.787 mm, slot volume = 500 nl, hydrophobic coated NEW

FP3NS500H

Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.914 mm, slot volume = 1,000 nl, hydrophobic coated NEW FP4NS1000H
Exposed pin length = 17 mm, overall pin length = 38.1 mm, pin diameter = 0.914 mm, slot volume = 2,000 nl, hydrophobic coated NEW FP4NS2000H
 
 


***NEW***
Hydrophobic Tall Slot Pins used in Double Float Plate Replicators

 
   
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = . 0.457 mm, slot volume = 10 nl, hydrophobic coated NEW

FP1TS10H

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = . 0.457 mm, slot volume = 20 nl, hydrophobic coated NEW

FP1TS20H

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = . 0.457 mm, slot volume = 30 nl, hydrophobic coated NEW

FP1TS30H

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = . 0.457 mm, slot volume = 40 nl, hydrophobic coated NEW

FP1TS40H

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 50 nl, hydrophobic coated NEW

FP1TS50H

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 50 nl, hydrophobic coated NEW

FP3TS50H

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 100 nl, hydrophobic coated NEW

FP3TS100H

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 150 nl, hydrophobic coated NEW

FP3TS150H

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 200 nl, hydrophobic coated NEW

FP3TS200H

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 300 nl, hydrophobic coated NEW

FP3TS300H

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, slot volume = 500 nl, hydrophobic coated NEW

FP3TS500H

Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.914 mm, slot volume = 1,000 nl, hydrophobic coated NEW FP4TS1000
Exposed pin length = 30 mm, overall pin length = 50.8 mm, pin diameter = 0.914 mm, slot volume = 2,000 nl, hydrophobic coated NEW FP4TS2000
 
 


Replacement Pins For Glass Slide Arrayer Replicators

 
   
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, slot volume = 6 nl FP1S6
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, solid pin hanging drop = ~20 nl  FP1
Exposed pin length = 28 mm, overall pin length = 62.2 mm, pin diameter = 0.457 mm, slot volume = 6 nl FP1S6T
Exposed pin length = 28 mm, overall pin length = 62.2 mm, pin diameter = 0.457 mm, solid pin hanging drop  = ~20 nl FP1TT
 
 


Solid Floating E-Clip Pins

 
 


We offer six styles of E-clip Pins: the FP pin which has a chamfered tip leading to a point and the FP6 which has the point cut off.  The solid FP pin delivers a smaller amount of liquid due to the point on the end of the pin.  It is well suited for making high density arrays on membranes and agar surfaces.  The flat tip FP6 pins are used to transfer larger volumes of liquid and the FP11, FP12, FP12A and FP13 which transfer the largest volumes are used by customers transferring yeast to agar plates. 

The FPFS2 pins have the E-Clip groove part way down the shaft to allow it to be used as a spring loaded floating pin useful in sampling frozen specimens.

The FP, FP6 styles of E-clip pins are the only ones that can be used with the manual style single float plate.   All the E-Clip pins (FP, FP6, FPAM, FPFS2, FP11A, FP12, FP12A and FP13 pin can be used with the Robot style double floating frames, or with double float frames that have been converted to manual Multi-Blot replicators with our conversion kits  (BGPK or BGPKL).

Click here for a list of published articles that cite using the products on this page.

 
 
 


Solid E-Clip Pin used in Single Float Plate Replicators

 
 
Exposed pin length = 23 mm, overall pin length = 34 mm, pin diameter = 1.58 mm, pointed tip, solid pin hanging drop = ~100 nl FP
Exposed pin length = 22 mm, overall pin length = 33 mm, pin diameter = 1.58 mm, flat tip, solid pin hanging drop = ~400 nl  NEW FP6
Exposed pin length = 22 mm, overall pin length = 27 mm, pin diameter = 3.18 mm, flat tip, solid pin hanging drop = ~3 ul NEW FP13
 
 

 
***NEW***
Slotted Floating E-Clip Pins

     
 


FLAT TIP SLOTTED E-CLIP PINS
 

Our new FP6 slotted flat tip E-Clip pins are the pins of choice for transferring liquids to agar or other soft surfaces and melting/transferring frozen stocks while replicating genomic/microbial libraries.

 


 

 
 
Exposed pin length = 22 mm, overall pin length = 33 mm, pin diameter = 1.58 mm, flat tip, slot volume = 0.5 ul NEW FP6S.5
Exposed pin length = 22 mm, overall pin length = 33 mm, pin diameter = 1.58 mm, flat tip, slot volume = 1.0 ul NEW FP6S
Exposed pin length = 22 mm, overall pin length = 33 mm, pin diameter = 1.58 mm, flat tip, slot volume = 2.0 ul NEW FP6S2
Exposed pin length = 23 mm, overall pin length = 33 mm, pin diameter = 1.58 mm, flat tip, slot volume = 5.0 ul NEW FP6S5
 
 


Pointed Tips

     
 


POINTED TIP SLOTTED E-CLIP PINS
 

Our pointed tip FP series slotted E-Clip pins are used to remove samples from the tips of conical wells of source plates.  Because of the sharp point they are not recommended for spotting on agar or other soft surfaces.

 

 
 


Pointed E-Clip Slot Pins used in Single Float Plate Replicators

 
 

VP 408FS.5, VP 408FS, VP 408FS2, VP 408FS5, VP 384FS.5, VP 384FS, VP 384FS2, VP 384FS5 

 
 
Exposed pin length = 23 mm, overall pin length = 34 mm, pin diameter = 1.58 mm, slot volume = 0.5 ul FPS.5
Exposed pin length = 23 mm, overall pin length = 34 mm, pin diameter = 1.58 mm, slot volume = 1 ul  FPS1
Exposed pin length = 23 mm, overall pin length = 34 mm, pin diameter = 1.58 mm, slot volume = 2 ul  FPS2
Exposed pin length = 23 mm, overall pin length = 34 mm, pin diameter = 1.58 mm, slot volume = 5 ul  FPS5
 
 


HYDROPHOBIC/LIPOPHOBIC COATED E-CLIP PINS AVAILABLE UPON REQUEST.

 
 

 
CUSTOM

 
 

If you don't see the pin size and length that you desire, please call us and we will custom make your pins.

     
 


Click here for a list of published articles that cite using the products on this page.

 
         

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