Full Text (pdf)
of Dispensing Using V&P Wands and Manifolds
Paper by Duong Chau V&P Scientific Staff Scientist
V&P Scientific wands and manifolds are designed for the purpose of aspirating
from microplates, washing microplates, and dispensing into microplates.
experiment was set up to determine the precision of the latter capabilities by
measuring the quantity of aqueous FITC dispensed per well and calculating the
coefficient of variations among the wells as well as among the dispenses.
The wands and manifolds range
between 4 and 48 tubes and are designed for microplates with wells between 48
and 1536. This assay evaluated the performance of four separate devices, the VP
180, VP 184 (96 well format), VP 189L, and VP 189M (384 well format); devices
that represent the fundamental design of the wands and manifolds. The
experiment also provided the data necessary to determine if the design
differences between wands and manifolds affect the dispense performance.
Dispensing was done in a non-contact fashion (above the
wells) and generally resulted in coefficient of
variations below 5% for all devices tested.
It was also observed that despite the differences
between the wands and manifolds in design, there were no
apparent differences in the quality of dispensing
capabilities. Only four devices out of many different
possible wand and manifold products V&P Scientific
carries were tested. However, this assay provides a
proof of principle for the design of the wands and
manifolds, thus similarly designed products are expected
to yield similar results.