Mono Pin Tool for Single Channel Pipetters on Automated Liquid Handlers

 
         
 

Another innovative tool from V&P Scientific, a Mono Pin Tool for cherry picking hits, in nanoliter volumes, from libraries in microplates.  After initial screening, the Mono Pin Tool allows for carrying out confirmation assay without going through a dilution process- which can be time consuming and use a lot of disposables.  Here is a simple and inexpensive solution for hit-picking using Automated Liquid Handlers (ALHs).

 

V&P has over 35 pins that cover volumes from 2 nl to 1000 nl.  Simply select one of those pins and match it up one of different floating fixtures (AFIX1FP_, AFIXT1FP_ or AFIXH1FP_) that corresponds to the pin diameter and which ALH is being used.

 

AFIX1FP Universal Mono Pin Tool, fits single channel pipetters on a variety of ALHs (top right photo)
AFIXT1FP Tecan Mono Pin Tool, fits single channel pipetter (LiHa) of Tecan EVO series ALHs (bottom right photo)
AFIXH1FP Hamilton Mono Pin Tool, fits single channel pipetter of Hamilton Star and Numbus series ALHs
AFIX1SFP Universal Mono Pin Tool, Short, fits single channel pipetters on a variety of ALHs where Z heigth clearance is an issue

 

 

         
 

Universal Mono Pin Tool

   
 

The Universal Mono Pin Tool was originally designed to fit over the Span-8 single channel pipetters on a Beckman FX, but as shown in the photos to the right it also attaches onto the single channel pipetter of a MWG RoboAmp 4200 or a Tecan EVO.  Many other automated liquid handlers have single channel pipetters are compatible with the MonoPin Tool.  Just slide the floating fixture over the single channel pipetter and tighten the set screws.  With a little programming the liquid handler is ready to cherry pick in nanoliter quantities.


If the single channel pipetter on a particular ALH does not accommodate the following specifications, V&P can make a custom Nanoliter Mono Pin Tool for that automated liquid handler.

 

Mono Pin Tool Fixture Specifications:
Length = 109 mm
Width = 8.5 mm (fits 9 mm spacing)
Thickness = 16 mm (accommodates set screws)
I.D. = 7.3 mm

 

Pins will protrude 12, 17 or 30 mm below the floating frame depending on which pin is selected.

 

AFIX1FP9
FP9 series pins: 0.229 diameter; FP9, FP9CB, FP9N, FP9T; solid, slot or hydrophobic coating; includes one pin
AFIX1FP8
FP8 series pins: 0.356 diameter; FP8, FP8CB, FP8N, FP8T; solid, slot or hydrophobic coating; includes one pin
AFIX1FP1
FP1 series pins:  0.457 diameter; FP1, FP1CB, FP1N, FP1T; solid, slot or hydrophobic coating; includes one pin
AFIX1FP3
FP3 series pins:  0.787 diameter; FP3, FP3CB, FP3N, FP3T; solid, slot or hydrophobic coating; includes one pin
AFIX1FP4
FP4 series pins:  0.914 diameter; FP4, FP4CB, FP4N, FP4T; solid, slot or hydrophobic coating; includes one pin

AFIX1FP6
FP6 series pins:  1.58 diameter; FP, FP6, solid, slot or hydrophobic coating; includes one pin
 

   

 
  Technical Documents:
    Changing Pin Holders in the Mono Pin Tool
 

 

 

Tecan Mono Pin Tool

   
 

The Mono Pin Tool for use on EVO Series Automated Liquid Handlers replaces the LiHa tip adapter on the single channel pipetter. This allows for more control over the positioning of the Mono Pin Tool for appilcations such as picking colonies from 1536 arrays.


The Tecan LiHa tip adapter is shown below left after removal from pipetter. The Mono Pin Tool is then attached carefully to the threaded end of the pipetter. The Mono Pin Tool is shown on the Tecan EVO LiHA single channel pipetter below and to the right.

   

AFIXT1FP1N
FP1 series pins:  0.457 diameter; FP1CB, FP1N, FP1T; solid, slot or hydrophobic coating; includes one pin

Mono Pin Tool for other pin diameters are available, please call V&P for information.

 

         
 

Hamilton Mono Pin Tool

   
 

The Mono Pin Tool for use on Star and Nimbus Automated Liquid Handlers makes use of the Hamilton CORE tip attachment technology to load the Pin Tool.  This allows for more control over the positioning of the Mono Pin Tool and for use of the Pin Tool “on the fly”.

 

AFIXH1FP1N
FP1 series pins:  0.457 diameter; FP1CB, FP1N, FP1T; solid, slot or hydrophobic coating; includes one pin

 

Mono Pin Tool for other pin diameters are available, please call V&P for information.

 

 

         
  Universal Mono Pin Tool For Z Height Limitations    
 


A shorter version of the Mono Pin Tool is available for Automated Liquid Handlers with a “Z” height  limitation. The length of this Mono Pin Tool is less that half the original, so Z clearance is no longer an issue. This is just another example of how V&P Scientific will adapt our products to meet your unique needs.

 

“Short” Mono Pin Tool Fixture Specifications:
Length = 40 mm
Width = 8.5 mm (fits 9 mm spacing)
Thickness = 16 mm (accommodates set screws)
I.D. = 7.3 mm


AFIX1SFP3N
FP3 series pins: 0.787 diameter; FP3CB, FP3N, FP3T; solid, slot or hydrophobic coating; includes one pin

 

Mono Pin Tool for other pin diameters are available, please call V&P for information.