Floating Pin Tool Replicators

     
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Defining a Pin Tool
Dry Plate Transfer
     


  Double Float Plate, Standard Tube Style Replicators

     
 

As the trend towards assay miniaturization continues with increasing demands to make nanoliter transfers, here is a simple way to convert either a 96 or a 384 Well Pipetting System into a pin tool work station that will deliver from 2 nl to 5 ul using 96, 384 or 1,536 pin tools.   We have developed 2 basic floating pin tool designs to provide nanoliter to microliter deliveries.  The E-Clip design covers the range of 0.5 to 5 ul and the Tube Style covers the range of 2 to 500 nl. 

This page describes Standard Tube Style Pin Tools which can be used with Beckman BioMek 2000 robots and they also can be converted to a manual mode with a conversion kit. These same pins and floating frame fixtures can also be mounted on 34 other robots by the use of a robot specific connecting mounting plate.  We have also made many custom mounting plates.  For example Harvard Medical School uses our Pin Tools on a Seiko robot.  

New - all floating frames sold after 8-8-2006 will be protected with our new Ni Lube corrosion resistant coating.  Ni Lube is a unique composite coating that blends the hardness and corrosion resistance of Nickel-Phosphorus with the low friction coefficient of Teflon. Submicron particles of Teflon are co-deposited with an electroless Nickel-Phosphorus alloy to provide various unique characteristics.  We have changed from conventional anodizing of the float plates because of corrosion effects of acid solutions on the anodized plates.  The new Ni Lube plates are gray in color and can be easily distinguished from the black anodized plates.  Although many of photos on the web site are of anodized (black) fixtures all future sales of floating fixtures will be plates with the new (gray) Ni Lube finish.

 
 


Click here for a list of published articles that cite using the products on this page.

 
 



The Double Float Plate style Replicators have several advantages over the E-Clip style Floating Replicators:

1.  The double float plate pins are smaller in diameter and deliver very small volumes (down to 2 nl).

2.  Smaller pin diameters also result in more precise delivery volumes (CV's ~4%).

3.  Because the pin is registered between 2 float plates over a greater height, the pin angle will be truer (closer to 90 degrees ) when they touch the surface. 

4.  The smaller hole on the lower float plate allows us to make very tight tolerances on the fit between the pin and the hole.  This results in very precise placement of the pins on the surface so that very high density arrays can be made without overlapping the spots.

5.  Precise spacing between absolutely straight small diameter pins is also essential when delivering liquid to or from 1536 well microplates.

The E-Clip Pin Tools on the other hand are capable of delivering larger volumes (from 0.5 to 5 ul).

 

 


  Floating Pins

     
 

Floating pins are not fixed in the base but will float when they hit the surface.  This ensures that all pins touch,  even if the surface is uneven.  The floating design can be used manually or also by robots where the Z control may vary.   The Tube Style series described on this page transports the smallest volume of liquid with very precise pitch location and absolute perpendicular vertical alignment.  The Standard Tube FP_ series pins are 50.8 mm long with an exposed pin length of 17 mm that is capable of being placed into microplate wells.  The Standard Tube Style can be used manually with a handle and Multi-Blot Guide Pin Assembly or it can be mounted directly to the Beckman BioMek 2000.  We also provide tube style pins of different lengths.  The FPC_ tube series is similar to the FP_ series Standard Tube Style but with a shorter overall length pin (33 mm) and a shorter 12 mm exposed pin length.  We recommend that you use the shorter FPC series tube pins on PerkinElmer PlateTrak, MiniTrak, EP3, Beckman MULTIMEK, Sagian MultiPette,   Standard Cybi Well and Matrix PlateMate work stations because of height limitation concerns.  The Deep well Cybi Well work stations will accommodate the longer FP_ and FP_T series pins.  If there are no height limitations we recommend the longer 50.8 mm pins (FP_ and FP_T series) because of the longer exposed pin length.  We have just introduced a new shorter overall length pin the FP(#N) series pins (17 mm exposed pin length and 38 mm overall)

 

The Standard Tube style pin tools are easily adapted to be mounted on many robot work stations.  We have made custom mounts for  custom robots and our customers have mounted our pin tools on the Beckman FX work stations.  We now have 2 mounting systems for the FX  and one for the FX Gripper If you want to use it with a robotic work station just fax us a drawing of the mating feature on your robot and we will incorporate that design into the mating plate.  All of our Standard Tube style pin tools have the mounting features for the Beckman BioMek 2000 already built in.  If you want to use it by hand, please add the BGPK kit to your order. Docking Stations for Pin Tools provide a safe facility for tool changing robots to park and pick up pin tools.

 
 


Save Money

     
 

If you have an obsolete 96 or 384 pipetting station or one with damaged pistons, you can bring it back on line as a 96, 384 or 1536 pin tool work station.  Not only will you save a ton of money rehabilitating an obsolete or broken work station,  you will also pay for the pin tool in your second screening operation by eliminating the cost of disposable pipet tips.  At $.12/tip, 200 cases of 384 tips are ~$9,000.00

 
 


For comprehensive data on nanoliter dispensing
with pin tools see the following pages.

 
 

Storage Stations for Pin Tools offer a safe place to store pin tools and keep them clean.

 


  Factors Affecting Pin Delivery Volumes

     
 

The list on below summarizes the factors that contribute to the volume delivered.  With each application these factors can be controlled and standardized so that the delivery volumes are very reproducible.  With most applications the CV's are less than 5%.

 

1. Pin Diameter
2. Surface tension of the liquid being transferred
3. Surface tension of the pin
4. Speed of removal of pin from source liquid
5. Speed of pin striking recipient dry plate
6. Depth to which the pin is submerged in source plate
7. Depth to which the pin is submerged in recipient plate
8. Volume of slot in pin
9. Surface tension of the dry plate and dwell time

     
 


V&P pins transfer liquid by carrying the liquid on the sides, tips and in slots we cut into the tips. The amount delivered is determined by the diameter and surface tension of the pin, the surface tension of the liquid, how far the pin is submerged into the liquid and the speed with which the pin is removed from the liquid.  When all of these variables are fixed, the accuracy of the liquid transfer is very consistent, with CV averages better than 5%.

Increasing the speed of withdrawal from the source plate by 7- fold will increase the volume delivered by as much as 3 fold in a linear relationship.  This phenomenon can be exploited to expand the range of delivery volume for a single pin. The table and the chart below illustrates the effects of increasing the speed 7- fold.  The speed range is from 0.78 cm/sec to 5.7 cm/sec.  Use this chart to select the pin that will deliver in your desired range.

   

 
  Select The Volume You Need To Transfer For Your Application

 
 
DMSO Liquid To Liquid Transfers With Uncoated Pins
     
   
Pin Diameter Shape Delivery Volume Range
FP9 0.229 mm Solid 5 - 15 nl
FP8 0.350 mm Solid 12 – 28 nl
FP1 0.457 mm Solid 19 –  68 nl
FP1S6 0.457 mm 6 nl Slot 26 – 74 nl
FP1S10 0.457 mm 10 nl slot 31 – 79 nl
FP1S20 0.457 mm 20 nl slot 43 – 89 nl
FP1S30 0.457 mm 30 nl slot 53 – 99 nl
FP1S40 0.457 mm 40 nl slot 64 – 109 nl
FP1S50 0.457 mm 50 nl slot 74 – 117 nl
FP3 0.787 mm Solid 84 – 240 nl
FP4 0.910 mm Solid 112 – 207 nl
FP 1.580 mm Solid Pointed 157 – 595 nl
FP3S100 0.787 mm 100 nl slot 178 – 326 nl
FP2S200 0.787 mm 200 nl slot 278 – 424 nl
FP6 1.580 mm Solid Flat 363 – 884 nl
FP3S500 0.787 mm 500 nl slot 527 – 656 nl
FPS.5 1.580 mm 0.5 ul slot 443 – 906 nl
FP6S.5 1.580 mm 0.5 ul slot 726 – 1,253 nl
FPS 1.580 mm 1 ul slot 938 – 1,424 nl
FP6S 1.580 mm 1 ul slot 1,239 – 1,786 nl
FPS2 1.580 mm 2 ul slot 1,817 – 2,248 nl
FP6S2 1.580 mm 2 ul slot 2,102 – 2,576 nl
FPS5 1.580 mm 5 ul slot 4,475 – 4,828 nl
FP6S5 1.580 mm 5 ul slot 4,475 – 4,828 nl
 
 


*Delivery volume range is determined by speed of withdrawal from source liquid.
slow speed  = 0.78 cm/sec = low volume delivery range
fast speed  =  5.70 cm/sec = high volume delivery range
Follow this link to assay methods

*Speed of withdrawal determines volume transferred. The faster the withdrawal speed the larger the volume transferred.  Withdrawal speed range is from 1.5 mm/sec to 30mm/sec.

 

*The volume of liquid in the source well also makes a significant difference in the volume transferred and there is a difference between sold and slotted pins.  The volume used for the 96 well data was 200 ul and the volume used for the 384 well data was 75 ul.  If you use more or less volume in your wells it will affect the volume transferred.  See this link for more information.

 

The links below are to delivery volume range tables for uncoated and hydrophobic coated delivering either DMSO or Aqueous solutions in liquid to liquid transfers.

DMSO TRANSFERS WITH HYDROPHOBIC COATED PINS

DMSO TRANSFERS WITH UNCOATED (STAINLESS STEEL) PINS

AQUEOUS TRANSFERS WITH HYDROPHOBIC COATED PINS

AQUEOUS TRANSFERS WITH UNCOATED (STAINLESS STEEL) PINS

 


  A Guide For Selecting The Right Pin For Your Application

 
 


1.  What volume do you need to  transfer?   DMSO? Aqueous? See these hyperlinked tables to determine your options.  Also consider custom slot pins.

2. Will the source plate have wells with significantly different levels of liquid?  (Cherry picked source plate or edge drying effect?).  If yes and if the absolute volume transferred is critical, then select the largest slot pin that is in your transfer range.  This will minimize the effect of liquid height on the volume of liquid carried on the sides of the pin.  Also consider custom slot pins.

3. Does the material transferred bind non-specifically to stainless steel?  If yes then select the Hydrophobic/lipophobic coated pins.  If no, select the uncoated pins.

4. What is the Z clearance on the robot deck from the highest impediment (top of the source plate/recipient plate/wash reservoirs, etc.) to the top of the robot mounting plate?  If it is greater than 77 mm you can use any of pins.  If it is less than 77 mm, you can only use the shorter FP(#)C series pins (12 mm exposed pin length), the FP(#N) series pins (17 mm exposed pin length) and the E-clip series pins (23 mm exposed pin length pins.  If the Z clearance is less than 60 mm, we recommend you contact us.  There are a few tricks we can do to shave off 5 to 10 mm in height. 

Links to the specifications of all the robot mounting plates/pin tool/pin combinations can be found on the height ("Z") restriction page

5.  Is the source plate a deep well plate?  If so you may need to use a 30 mm long exposed length pin ("T" pin) to reach down to the lower levels of the well. 

6.  Should you choose the solid pin versus the slot pin? Although there appears to be a slight advantage for slot pin, CV's between the two pins are very good.  Both are easy to clean between specimens.  Biggest factor is cost.  If you don't need to deal with varying liquid heights and you can obtain the volume necessary with a solid pin, choose the economical solid pin.

7.    If you are still uncertain about which pin to select for your application, you can perform a simple "Proof of Principle" test with several different pins using our inexpensive VP 450FP3 Replicator Strip coupled via a VP 452MP to one of our robot mounting plates.   An even simpler solution is to use a work station with a 1, 4 or 8 probe dispense head with the new V&P Mono Pin Tool to test the various pins.

 


  Liquid To Dry Plate Transfers For Multiplexing Assays

     
 


Many of our customers want to make liquid to dry plate transfers to make mini-microarrays in a single well for multiplexing assays.  By their very nature these transfers only leave a small volume remaining on the plate.  The amount remaining depends on the surface tension of the plate, the transfer liquid and the pin (diameter and shape).  We have found that reasonable results can be obtained if you use an aqueous spotting buffer and a polypropylene surface (CV's less than 9%).  DMSO spotting buffers leave much less liquid on the dry plate and when used with polystyrene plates the CV's are significantly higher. 

The plate to the right was spotted using a 50 nl slot pin (FP1CS50) transferring oligo DNA in an aqueous spotting buffer to a specially treated polypropylene plate.  Our customer in this case wanted to place 42 individual spots in the bottom of each well of a 96 well microplate.  Spot diameter = 0.55 mm. Volume transferred to the spot = 5.85 nl

The photo on the right is the result of a four fold serial dilution hybridization assay of the Oligo spots with a labeled DNA that can be detected optically and quantitated.

Follow this link for more information on liquid to dry transfers and Multiplexing.

 

 


  Defining a Pin Tool

     
 


Originally we sold our pin tools with unique part numbers.  As we have added more pins to our selection and more robot systems to mount the pin tools to, the number of unique part numbers grew exponentially to an un-manageable size.  Our solution has been to specify a pin tool by the 3 component parts that compromise the Pin Tool.  The Pins, the Floating Fixture and the Robot Mounting Plate.  If you have previously ordered a pin tool by unique part number, we will be able to provide the same pin tool to you.

 








 


  Selecting a Pin Tool as Simple as 1, 2, 3

   
 


1. Select the appropriate pin. 
For the manual and Beckman Bio Mek 2000 robots we recommend the longer FP_ series pins.

2. Select the Floating Fixture with the appropriate pattern (96, 384 or 1,536) and diameter of holes.

3. Select the robot system you wish to use the Pin Tool with.  We have made Mounting Plates to over 34 Robot Workstations.  The BMP Mounting Plate on this page accommodate the manual pin tool and the Beckman BioMek 2000 robot.

Determine the weight of a Robot pin tool at this link

 

   


  Convert a Robot Pin Tool to a Manual Pin Tool

     
 


Our Robotic floating Pin Tools can easily be converted to a manual pin tool with this simple BGPK  kit which contains a Guide Pin Frame and handle and sturdy mounting plate (BMPM)  The guide pins are used with our manual microplate (VP 381N) and membrane registration accessories (VP 382 and VP 382B) to keep the pins in the center of the wells and to make high density arrays on membranes or agar surfaces

Conversion of robot pin tools to manual Multi-Blot Replicators is very easy using our BGPK for Standard length pins and the BGPKL for longer pins.  Simply remove the robot mounting feature from the float plates disassemble the pin tool and reassemble it with the Guide Pin Frame as part of the tool.  Attach the Manual Mounting Plate and handle and you are ready to go.   If this sounds too daunting for you - send it back to us and we will do it for you for a nominal fee.

The main reason for this conversion is to attach  guide pins to the replicator so it can be aligned to Microplate registration accessories such as the VP 381 series Library copiers for replicating and reformatting genomic libraries, VP 380 series colony copiers to make high density arrays on agar surfaces or  VP 382 membrane printers to make high density arrays on nylon or other membranes.

The BGPKL kit is used when longer replicator pins are used like the FPAM pins which are 63 mm long.   A pin tool with FPAM pins is illustrated in the photo to the right.   In order to accommodate these longer pins we need to use a longer and more sturdy guide pin found on the BGPL frame.  These larger guide pins are 3.17 mm in diameter.

Each of the kits on the right (BGPKL and BGPK) is made up of a handle, a manual mounting plate and a guide pin frame.  The guide pin frame is the only  difference between the two kits

 

 
 


For comprehensive data on nanoliter dispensing
with pin tools see the following pages.

 
       


  Standard 96 Pin Double Float Plate Style Replicators

     
 


0.229 mm Diameter Pins To Deliver ~4 nl liquid to liquid

     
 


The 0.229 mm diameter stainless steel pins are 50.8 mm long with flat tips and are fixtured inside a 1.58 mm diameter tube 31.75 mm long thus strengthening the pin characteristics and adding mass to enhance the floating ability.  This pin/tube combinations passes through two float plates 2.54 cm apart for greater precision of spot placement.  As the Replicator is lowered onto the gridding surface, each pin rides on top of the lower float plate assembly until it touches on the gridding surface and delivers ~2 nl of liquid in the hanging droplet at the tip.  When the tip hits the surface the pin slides up ("floats") up through the holes.  This floating action assures that each of the pins will deliver its droplet even if the blotting/gridding surface is not level or smooth.  These Replicators are designed to make multiple offset blots to produce high density arrays.  Just fax us a drawing of the mating feature on your robot and we will incorporate that design into the mating plate.

     
 


0.457 mm Diameter Pins To Deliver ~20 nl liquid to liquid

     
 


The 0.457 mm diameter stainless steel pins are 50.8 mm long with flat tips and are fixtured inside a 1.58 mm diameter tube, 31.75 mm long thus strengthening the pin characteristics and adding mass to enhance the floating ability.  This pin/tube combinations passes through two float plates 2.54 cm apart for greater precision of spot placement.  As the Replicator is lowered onto the gridding surface, each pin rides on top of the lower float plate assembly until it touches on the gridding surface and delivers ~6 nl of liquid in the hanging droplet at the tip.  When the tip hits the surface the pin floats up through the holes.  This floating action assures that each of the pins will deliver its droplet even if the blotting/gridding surface is not level or smooth.  These Replicators are designed to make multiple offset blots to produce high density arrays.  The VP 438FP1 is designed to be used with a robotic work station or by hand as shown in the photo to the right.  If you want to use it manually we need to equip the pin tool with a guide pin assembly and handle.  Please specify manual or robotic use.  If you want to use it with a robotic work station just fax us a drawing of the mating feature on your robot and we will incorporate that design into the mating plate.

 
 


0.787 mm Diameter Pins To Deliver ~80 nl liquid to liquid

     
 


The 0.787 mm diameter stainless steel pins are 50.8 mm long with flat tips and are fixtured inside a 1.58 mm diameter tube, 31.75 mm long thus strengthening the pin characteristics and adding mass to enhance the floating ability.  This pin/tube combinations passes through two float plates 2.54 cm apart for greater precision of spot placement.  As the Replicator is lowered onto the gridding surface, each pin rides on top of the lower float plate assembly until it touches on the gridding surface and delivers ~35 nl of liquid in the hanging droplet at the tip.  When the tip hits the surface the pin floats up through the holes.  This floating action assures that each of the pins will deliver its droplet even if the blotting/gridding surface is not level or smooth.  These Replicators are designed to make multiple offset blots to produce high density arrays.  The VP 438FP3 is designed to be used with a robotic work station or by hand.  If you want to use it with a robotic work station just fax us a drawing of the mating feature on your robot and we will incorporate that design into the mating plate.

 


Click here for a list of published articles that cite using the products on this page.

     

 
  384 Double Float Plate Replicators

     
 


0.457 mm Diameter Pins To Deliver ~20 nl liquid to liquid

     
 


The stainless steel pins in the VP 384FP1 have a diameter of only 0.457 mm and will deliver a ~6 nl hanging drop. This small diameter pin enables a smaller delivery volume.  Each 50.8 mm long pin is fixtured inside a 1.58 mm diameter tube that is 31.75 mm long, thus strengthening the pin design and adding mass to enhance the floating characteristics.  Each pin slides ("floats") through holes in the lower and upper float plates as it touches on the bottom of the microplate well or the gridding surface.  This floating action assures that each of the pins will touch and deliver its droplet even if the blotting/gridding surface is not level or smooth. 

The VP 384FP1 is designed to be used with a robotic work station or by hand.  Just fax us a drawing of the mating feature on your robot and we will incorporate that design into the mating plate.

Dimensions: 85.75 mm wide, 135.42 mm long, 67 mm high from tip of the pins to the top of the mating plate and weighs 543 grams. Exposed pin length = 17.4 mm, pin diameter = 0.457 mm .....VP 384FP1

 

The 0.229 mm diameter stainless steel pins are 50.8 mm long with flat tips and are fixtured inside a 1.58 mm diameter tube, 31.75 mm long, thus strengthening the pin characteristics and adding mass to enhance the floating ability.  This pin/tube combinations passes through two float plates 2.54 cm apart for greater precision of spot placement.  As the Replicator is lowered onto the gridding surface, each pin rides on top of the lower float plate assembly until it touches on the gridding surface and delivers ~2 nl of liquid in the hanging droplet at the tip.  When the tip hits the surface the pin floats up through the holes.  This floating action assures that each of the pins will deliver its droplet even if the blotting/gridding surface is not level or smooth. These Replicators are designed to make multiple offset blots to produce high density arrays.  The VP 384FP9 is designed to be used with a robotic work station or by hand.  Just fax us a drawing of the mating feature on your robot and we will incorporate that design into the mating plate.


Click here for a list of published articles that cite the products on this page.

 


 


  1536 Floating Pin Replicators

     
 


0.457 mm Diameter Pins To Deliver ~20 nl liquid to liquid

     
 


The stainless steel pins in the VP 838FP1 have a diameter of only 0.457 mm and will deliver a   ~6 nl hanging drop.  This small diameter pin eliminates "well wall clearance" problems encountered with larger diameter pins with some brands of 1536 well microplates.   Each 50.8 mm long pin is fixtured inside a 1.58 mm diameter tube that is 31.75 mm long, thus strengthening the pin design and adding mass to enhance the floating characteristics.  Each pin slides ("floats") through holes in the lower and upper float plates as it touches on the bottom of the microplate well or the gridding surface.  This floating action assures that each of the pins will touch and deliver its droplet even if the blotting/gridding surface is not level or smooth.   

 

The VP 838FP1 is designed to be used with a robotic work station or by hand.  Just fax us a drawing of the mating feature on your robot and we will incorporate that design into the mating plate.

 

The VP 838FP1 Replicator can even be used manually to simultaneous transfer 20 nl of specimen to the 2 nl wells of the Corning 1536 assay plate.  The ability to simultaneous start all assays at the same time is critical in many assay procedures.  The guide pins on the VP 838FP1 and the alignment holes on our VP 381F LIBRARY COPIER™ provide a precise registration of the Replicator to the Microplate.  This is critical with the small diameter 1,536 wells. 

 

Dimensions: 85.75 mm wide, 135.42 mm long, 67 mm high from tip of the pins to the top of the mating plate and weighs 1800 grams.

 

 

 

 

 

 

 

 

The 25.4 mm alignment distance between the lower and upper float plates insures that pin to pin spacing remains true at 2.25 mm. The evenness of this spacing is demonstrated in the blot to the right.

 

 

 


Click here for a list of published articles that cite the products on this page.

 

 
 


1536 Pin Replicators

     
 


If you are transferring DNA, cDNA or other materials in hydrophobic solutions you will find that using our new V&P Pin Cleaning Solution will greatly increase the precision of delivery.  For an explanation see the FAQ page and the V&P Pin Cleaning Solution page.

Replacement Pins for the above 96, 384 and 1536 Solid Pin Replicators can purchased inividually.

     


  Hydrophobic & Liphobic Coating Of Pins

     
 


All of the above pins can also be obtained with a hydrophobic/lipophobic coating to reduce non-specific binding by proteins and lipoproteins.  Just adding an H to the above pin designates that pin as a hydrophobic/lipophobic coated pin.  This coating is metallic and only a few angstroms thick so it does not effect the basic dimensions of the pins.  These coated pins are very useful in handling solutions with proteins or lipids that may stick to naked stainless steel.  Click on the following hyperlink for more details on the nature of our hydrophobic/lipophobic coating and the coating process.

     
 


Hydrophobic Pins used in the Standard Double Float Plate Replicators 

     
 


Replacement pins are also available on our Replacement Pin page. Please visit this hyperlink for Pin Tool Data And Charts.

     


  Floating Slot Pin Replicators

     
 

Slot pins have several advantages over solid pins:

  1. Slot pins carry a larger volume than solid pins in liquid to liquid transfers.

  2. Slot pins are not as sensitive to the depth of the mother liquid as solid pens.

  3. Slot pins deliver a larger volume than solid pins in liquid to dry plate transfers.

  4. Slot pins deliver a larger volume than solid pins in liquid to membrane transfers.

  5. Slot volume is customizable.

  6. Slot pins can deliver to dry plates by pre-wetting the plate. See customer data.

Replacement Pins for the 96 and 384 Slot Pin Replicators.

 
 
     
 


  Hydrophobic Slot Pin Replicators

     
 


Hydrophobic/lipophobic coated pins are very useful in handling solutions with proteins or lipids that may stick to naked stainless steel. Click on the following hyperlink for more details on the nature of our hydrophobic/lipophobic coating and the coating process. Visit the following link for Pin Tool Data and Charts.

 

Replacement Pins for the 96 and 384 Hydrophobic Slot Pin Replicators.

     


  Cleaning The Pins

     
 


Procedures for transferring liquids, robot speed, wash solutions, blotting protocols and pin cleaning can found at this hyperlink.  Cleaning the pins between mother plates is easy and effective.  In an experiment to determine the amount of carry over after 100 transfers of concentrated FITC (mother to daughter plate) we were only able to detect  0.000,001,8% of the residual FITC on the pin using only two 100 ml wash baths.

 

 
 


Download Video of 384 Pin Tool on a Maxim Robot
(mpeg movie- 6.2MB)

 

Press the play button to view the video in a flash format, flash must be installed, or click the download link to download a mpeg version.

 

   


  Custom Floating Slot Pin Replicators

     
 


Custom Floating Slot Pin Replicators can also be made using the 0.457 mm and 0.787 mm diameter pins and placing them into the Floating Pin Replicators described above.  The slot volumes between these two pins can cover a range of volumes from 5 nl to 1000 nl by changing the width and depth of the slot.  Inquire by phone or e-mail for more information.

     
 


Replacement Pins

     
 

Replacement Pins for all the replicators on this page can be found on this hyperlink.

     


  Cleaning Pins on Robot Mounted Replicators

     
 


The most important aspect of using pin tools is to start with clean pins and then keep them clean so they will consistently deliver the same volume.  We recommend our Pin Cleaning Solution (VP 110) to clean the pin of protein and other residue buildup before you start an assay.  To quickly and efficiently remove the previous transfer from the pin we have several different wash reservoirs, flowing reservoirs and fountains to clean  96, 384 and 1536 floating pin tools.  These wash stations also are adapted so the blotting station is part of the washing station thus reducing the cleaning cycle time and the number of robot stations required for the cleaning process.  We also have other independent blotting stations (VP 540D)

Integral to the cleaning process of all pins is the blotting step between baths.  Lint particles in the slots or on pin tips, from standard paper towels, will interfere with the loading and unloading of liquid.  We have solved this problem.  The VP 540DB blotting system is a tray with the standard microplate foot print that contains a super absorbent polypropylene pad covered by a Lint Free Blotting Paper.  We strongly recommend that you use this or our other lint free blotting systems.

 

 


  Manual Membrane Macro Array Starter Kit Accessories for FP Series

 
 


For your convenience and a 5% discount, V&P Scientific has combined the essential components of our floating FP series (FP1, FP3, and FP9)  96, 384 and 1536 pin MULTI-BLOT™ Replication System into a Starter Kit to make up to 4 identical 864 or 1536 feature Macro Arrays at once.  You select whichever Solid or Slot Pin FP series MULTI-BLOT™ Replicator that fits your application and delivery volume,  then just add it to the Basic Macro Array Starter Kit VP OK14A and you have everything you need to start making arrays.  This kit differs only from the VP OK14 in that a VP 381N LIBRARY COPIER™ is substituted for the VP 381 because the pins on the FP Series Replicators are shorter and therefore need a shorter registration accessory.  Included in the Starter Kit are the following:

 
 


VP OK14A

Four, MULTI-PRINTS
One, LIBRARY COPIER™
One, V&P Pin Cleaning Solution Bottle
One, Package Of Lint Free Blotting Paper
One, Pyrex Alcohol Reservoir With Lid
One, Clear Overlay Reading Mat For 864 & 1536 Arrays
Five, Plastic Bleach Or Water Reservoirs
One, Pin Cleaning Brush
One, 30 cm by 3 meter Roll Of Hybond N Nylon Membrane
VP 382
VP 381N
VP 110
VP 522
VP 420
VP 430
VP 421
VP 425
VP 503
     


  Accessories

     
 


We suggest you buy a few inexpensive accessories that will make the Replicators more useful, easier, safer and more reproducible. 

The VP 381N is a Short Registration Device for the Double Float Plate Replicators and their shorter exposed length pins.  Because the pins are shorter the registration device must be shorter.  This device aligns the Replicator pins to the center of the wells.  This is very important as you do not want the pins to touch the side of wells as you remove the pins because that will knock off the hanging drop at the pin tip.  The VP 381N LIBRARY COPIER™ is also used with our 96 Pin Double Float Plate Replicators to re-array from four, 96 well plates to one, 384 well plate or vice versa, to break out samples from one, 384 well plate to four, 96 well plates.

The VP 381F is also a Short Registration Device for the Double Float Plate Replicators and their shorter exposed length pins.  The advantage of the VP 381F is that it is easier and faster to replicate libraries or make serial dilutions.

The VP 382 MULTI-PRINT™ is a registration device that allows you to make membrane arrays in an area the size of a standard microtiter plate with our Replicators.  Either four, 384 well plates or nine, 96 well plates can be arrayed into a 1536 or 864 array on the membrane.

After arraying from four, 384 well plates to one, 1536 membrane array (or nine, 96 well plates to one, 864 membrane array), the VP 430 Reading Mat helps locate which mother plate well produced a positive spot on the membrane array.

The VP 420 is a Glass Alcohol Reservoir that is used in the sterilization of the Replicators by flaming.  Some labs use plastic reservoirs, but if flaming alcohol drops into them the plastic burns, dumping flaming alcohol all over the counter.  This covered dish is also useful for storing the alcohol as well as VP 110 Pin Cleaning Solution (below) for reuse.  

The VP 425 is a Pin Cleaning Brush that is used to keep the Replicator pins clean so they will carry the same amount of liquid each time.   

Our VP 110 is a Pin Cleaning Solution that is used to clean and treat the pin tips when they can't be flamed.  

Integral to the cleaning process of all pins is the blotting step between baths.  Lint particles in the slots or pin tips, from blotting onto standard paper towels, will interfere with the loading and unloading of liquid.  The VP 522 is a Lint Free Blotting Paper that doesn't transfer lint particles to the pins as many blotting papers or paper towels can do.  We strongly recommend that you use these blotting pads placed over several layers of paper towels. 

These accessories are also found in the Basic Membrane Array Started Kit VP OK14A at a 5% discount. 

 


  Robot Pin Tool Accessory Kit

     
 


We highly recommend that you order the Robot Pin Tool Accessory kit at the time you order the pin tool.  This "essentials" accessory kit contains the VP 540DB Lint Free Blotting System used to clean the pins efficiently between samples, two VP 540 Wash Reservoirs to wash the pins between source plates, the VP 110 Pin Cleaning Solution is used the clean the pins daily, the VP 425 Pin Cleaning Brush is used if a build up occurs and the VP 555 Safe Pin Tool Storage System is used to keep it clean and the fragile pins out of harms way.   Although we have several other wash and blot accessories available, these are the most cost effective for the majority of applications.  If you elect to use the fountain and flowing reservoirs we still strongly recommend the other items in this kit.  When purchased as the VP OK17 Kit you will receive a 5% discount over the sum of the individual items.

 
   

Robot Pin Tool Accessory Kit - VP OK17

Lint Free Blotting System with pad (1) VP 540DB
Pin Wash Reservoirs (2) VP 540
Pin Cleaning Solution (1) VP 110
Pin Cleaning Brush (1) VP 425
Safe Pin Tool Storage Station (1) VP 555
     
 

Click here for a list of published articles that cite using the products on this page.
 
         

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