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Manual Pin Tools Robot Pin Tools Floating Fixtures Robot Mounts |
Accessories Reagent Reservoirs Pin Cleaning |
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NEW RESEARCH In addition to transferring liquids, these floating pin E-clip style replicators have been used to precisely scratch a limited area of a tissue culture monolayer in 384 well microplates. The perturbed area can be then screened for effects with a large number of variables quantitatively. Over 10,000 compounds, small molecules or siRNAs can be screened in a single day. Yarrow et. al. 2004 describe the methodology behind these wound healing experiments. The size of the wound made in the monolayer is determined by the diameter of the contact tip of the pin and length of the stroke. We have made several Library copiers with oval alignment holes that aid in the making of precise areas of wounding. For more information follow this link. We offer 24, 48 , 96 and 384 floating pin tools for these wounding experiments. Contact us for help with your specific application. New - all floating frames sold after 8-8-2006 will be protected with our new Ni Lube corrosion resistant coating. Ni Lube is a unique composite coating that blends the hardness and corrosion resistance of Nickel-Phosphorus with the low friction coefficient of Teflon. Submicron particles of Teflon are co-deposited with an electroless Nickel-Phosphorus alloy to provide various unique characteristics. We have changed from conventional anodizing of the float plates because of corrosion effects of acid solutions on the anodized plates. The new Ni Lube plates are gray in color and can be easily distinguished from the black anodized plates. Although many of photos on the web site are of anodized (black) fixtures all future sales of floating fixtures will be plates with the new (gray) Ni Lube finish.
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We offer FPAM pins of longer lengths for scientists using deep well blocks. The FPFS2 pins have the E-Clip groove part way down the shaft to allow it to be used as a spring loaded floating pin useful in sampling frozen specimens. The FP pointed pin version tapers from 1.58 mm to a 0.38 mm flat spot. It was originally developed to deliver small volumes (~100 nl) to facilitate making moderate density (864 colony) arrays on agar surfaces. The FP6 flat tip (1.58 mm flat tip) version was developed to to transport larger volumes (~400nl) to membranes and agar surfaces. The 96 replicator version that uses the new flat pin is the VP 408FP6. We have recently introduced three new larger diameter pins (2.36 mm -- FP12 and FP12A, 3.18 mm --FP13 and 4.77 mm -- FP11) E-Clip floating pins for customers who wanted to transfer larger inoculums of yeast cultures to agar filled Omni Trays. |
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If you don't have a robot you can purchase the new V&P Pin Tool Robot and attach any of our pin tools to it. This gives you the advantages of robotic reproducibility at a fraction of the cost of large robotic system. |
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The list on below summarizes the factors that contribute to the volume delivered. With each application these factors can be controlled and standardized so that the delivery volumes are very reproducible. With most applications the CV's are less than 5%.
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3.18 mm diameter pins to deliver ~3 ul hanging drops |
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The 1.58 mm diameter stainless steel pins are fixtured with an E-Clip to rest on a single float plate. The other end of the pin is chamfered to a 0.38 mm flat spot on the tip. As the Replicator is lowered onto the gridding surface each pin rests on the single float plate until it touches on the gridding surface and delivers ~0.1 ul of liquid in the hanging droplet at the tip. When the tip hits the surface, the pin floats up through the holes. This floating action assures that each of the pins touches the surface and will deliver its droplet even if the blotting/gridding surface is not level or smooth. These Replicators are designed to make multiple offset blots to produce moderate density arrays (864 spots/array). They are very cost effective and the pins can be easily replaced. Also it is possible for the customer to purchase slot pins with different dispense volumes and place them into the Replicator to fit assay requirements. The system is simple enough that the customer can make these changes. |
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96 pins, height of Replicator = 48.7 mm,
exposed pin length = 23 mm, pin diameter = 1.58 mm |
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The VP 407FP12 is designed to be used by hand. it comes complete with a BMPM and 96 FP12 pins. The 2.36 mm diameter stainless steel FP12 pins have flat tips. As the Replicator is lowered onto the gridding surface, each pin rests on a single float plate assembly until it touches on the gridding surface and delivers ~1.5 ul of liquid in the hanging droplet at the tip. When the tip hits the surface the pin floats up through the holes. This floating action assures that each of the pins will deliver its droplet even if the blotting/gridding surface is not level or smooth. |
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The VP 407AFP13 is designed to be used by hand. It comes complete with a BMPM and 96 FP13 pins. The 3.18 mm diameter stainless steel FP13 pins have flat tips. As the Replicator is lowered onto the gridding surface, each pin rests on a single float plate assembly until it touches on the gridding surface and delivers ~3 ul of liquid in the hanging droplet at the tip. When the tip hits the surface the pin floats up through the holes. This floating action assures that each of the pins will deliver its droplet even if the blotting/gridding surface is not level or smooth. A 48 pin version of this pin tool is also available. |
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The 1.58 mm diameter stainless steel pins are chamfered to a 0 .38 mm flat spot on the tip. As the Replicator is lowered onto the gridding surface, each pin rests on a single float plate assembly until it touches on the gridding surface and delivers ~0.1 ul of liquid in the hanging droplet at the tip. When the tip hits the surface the pin floats up through the holes. This floating action assures that each of the pins will deliver its droplet even if the blotting/gridding surface is not level or smooth. These Replicators are designed to make two offset blots to produce moderate density arrays (768 spots/array). |
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If
you want to transfer larger volumes such as a yeast culture to an agar surface
we suggest you use our flat tip FP6 pin version of the replicator
....VP 384FP6 |
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The exposed pin length of the FPAM pins can easily be adjusted by adjusting the height of standoff spacer between the two float plates as illustrated in the photo to the right. |
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Slot pins have several advantages over solid pins:
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A significant advantage of the slot pin style is that assures a greater precision in the delivery of the liquid as illustrated in the following table. For Pin Tool Data And Charts Please Visit This Hyperlink See this link for data on
DMSO and Aqueous delivery with
hydrophobic coated and uncoated solid and slot pins |
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The VP 382 MULTI-PRINT™ is a registration device that allows you to make membrane arrays in an area the size of a standard microtiter plate with our Replicators. Either four, 384 well plates or nine, 96 well plates can be arrayed into a 1536 or 864 array on the membrane. After arraying from four, 384 well plates to one, 1536 membrane array (or nine, 96 well plates to one, 864 membrane array), the VP 430 Reading Mat helps locate which mother plate well produced a positive spot on the membrane array. The VP 420 is a Glass Alcohol Reservoir that is used in the sterilization of the Replicators by flaming. Some labs use plastic reservoirs, but if flaming alcohol drops into them the plastic burns, dumping flaming alcohol all over the counter. This covered dish is also useful for storing the alcohol as well as VP 110 Pin Cleaning Solution (below) for reuse. The VP 425 is a Pin Cleaning Brush that is used to keep the Replicator pins clean so they will carry the same amount of liquid each time. Our VP 110 is a Pin Cleaning Solution that is used to clean and treat the pin tips at the beginning of the day. Integral to the cleaning process of all pins is the blotting step between baths. Lint particles in the slots or pin tips, from blotting onto standard paper towels, will interfere with the loading and unloading of liquid. The VP 522 is a Lint Free Blotting Paper that doesn't transfer lint particles to the pins as many blotting papers or paper towels can do. We strongly recommend that you use these blotting pads placed over several layers of paper towels or our super absorbent VP 540DB polypropylene pad/tray system. These accessories are also found in the Basic Membrane Array Starter Kit VP OK14A at a 5% discount. |
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An E-Clip robot pin tool consists of three separate parts. 1. The pins, (#FP, FP6 or
slotted versions) For example the part numbers for pin tool in the photos to the right would be FP6S5, AFIX96FP6 and BMPMULTIMEK. To order, just tell us those 3 part numbers and we will assemble and price your Pin Tool. Determine the weight of a Robot pin tool at this link |
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Each of the kits on the right (BGPKL and BGPK) is made up of a handle, a manual mounting plate and a guide pin frame. The guide pin frame is the only difference between the two kits |
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The main reason for this conversion is to attach guide pins to the replicator so it can be aligned to Microplate registration accessories such as the VP 381 series Library copiers for replicating and reformatting genomic libraries, VP 380 series colony copiers to make high density arrays on agar surfaces or VP 382 membrane printers to make high density arrays on nylon or other membranes. The BGPKL kit is used when longer replicator pins are used like the FPAM pins which are 63 mm long. A pin tool with FPAM pins is illustrated in the photo to the right. In order to accommodate these longer pins we need to use a longer and more sturdy guide pin found on the BGPL frame. These larger guide pins are 3.17 mm in diameter. Each of the kits on the right (BGPKL and BGPK) is made up of a handle, a manual mounting plate and a guide pin frame. The guide pin frame is the only difference between the two kits Some of our customers wanted to transfer organisms from a deep well microplate to an agar Omni Tray (VP 413). Using our 45 mm exposed length FPAM pins we can reach the bottom of all conventional deep well microplates and still float lightly on an uneven agar surface to make sure that every pin contacts the surface. The floating pin system assures that every pin contacts the agar surface and the inoculum is transferred uniformly. We are able to use the BGPKL kit and interchangeably use this pin tool with robots and/or manually. |
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We highly recommend that you order the Robot Pin Tool Accessory kit at the time you order the pin tool. This "essentials" accessory kit contains the VP 540DB Lint Free Blotting System used to clean the pins efficiently between samples, two VP 540 Wash Reservoirs to wash the pins between source plates, the VP 110 Pin Cleaning Solution is used the clean the pins daily, the VP 425 Pin Cleaning Brush is used if a build up occurs and the VP 555 Safe Pin Tool Storage System is used to keep it clean and the fragile pins out of harms way. Although we have several other wash and blot accessories available, these are the most cost effective for the majority of applications. If you elect to use the fountain and flowing reservoirs we still strongly recommend the other items in this kit. When purchased as the VP OK17 Kit you will receive a 5% discount over the sum of the individual items |
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Copyright © 2007, V&P Scientific, Inc. All rights reserved.