Floating Pin Replicator, E-Clip Style

     
 
Manual Pin Tools
Robot Pin Tools
Floating Fixtures
Robot Mounts

Accessories
Reagent Reservoirs
Pin Cleaning
     
 


Floating pins are not fixed in the base but will float when they hit the surface.  This ensures that all pins touch,  even if the surface is uneven.  We offer 2 Floating Frame Fixtures for the E-clip pins.  The first was designed for manual use and consists of a single solid Floating Frame Fixture.  The second or Pin Tool design has 2 floating frames and is used for robot applications and is capable of being mated to over 37 different robot work stations.   If you wish to mount on a robot system you must also purchase the robot mounting plate separately.

NEW RESEARCH In addition to transferring liquids, these floating pin E-clip style replicators have been used to precisely scratch a limited area of a tissue culture monolayer in 384 well microplates.  The perturbed area can be then screened for effects with a large number of variables quantitatively.  Over 10,000 compounds, small molecules or siRNAs can be screened in a single day. Yarrow et. al. 2004  describe the methodology behind these wound healing experiments.  The size of the wound made in the monolayer is determined by the diameter of the contact tip of the  pin and length of the stroke.  We have made several Library copiers with oval alignment holes that aid in the making of precise areas of wounding.  For more information follow this link.

We offer 24, 48 , 96 and 384 floating pin tools for these wounding experiments.  Contact us for help with your specific application. 

New - all floating frames sold after 8-8-2006 will be protected with our new Ni Lube corrosion resistant coating.  Ni Lube is a unique composite coating that blends the hardness and corrosion resistance of Nickel-Phosphorus with the low friction coefficient of Teflon. Submicron particles of Teflon are co-deposited with an electroless Nickel-Phosphorus alloy to provide various unique characteristics.  We have changed from conventional anodizing of the float plates because of corrosion effects of acid solutions on the anodized plates.  The new Ni Lube plates are gray in color and can be easily distinguished from the black anodized plates.  Although many of photos on the web site are of anodized (black) fixtures all future sales of floating fixtures will be plates with the new (gray) Ni Lube finish.


Click here for a list of published articles that cite using the products on this page.

 


  E-Clip

     
 


The E-Clip style Floating Replicators described on this page are the least expensive and transport the largest volume of liquid per pin. The robot Floating Frame Fixture designs are compatible with all the pin styles and the manual (single float plate) only with the FP and FP6 the E-Clip pin versions.

We offer FPAM pins of longer lengths for scientists using deep well blocks.  The FPFS2 pins have the E-Clip groove part way down the shaft to allow it to be used as a spring loaded floating pin useful in sampling frozen specimens.

 The FP pointed pin version tapers from 1.58 mm to a 0.38 mm flat spot.  It was originally developed to deliver small volumes (~100 nl) to facilitate making moderate density (864 colony) arrays on agar surfaces.  The FP6 flat tip (1.58 mm flat tip) version was developed to to transport larger volumes (~400nl) to membranes and agar surfaces.  The 96 replicator version that uses the new flat pin is the VP 408FP6.

We have recently introduced three new larger diameter pins (2.36 mm -- FP12 and FP12A, 3.18 mm --FP13 and 4.77 mm -- FP11) E-Clip floating pins for customers who wanted to transfer larger inoculums of yeast cultures to agar filled Omni Trays.

 

 


  Factors Affecting Pin Delivery Volumes

     
 

The list on below summarizes the factors that contribute to the volume delivered.  With each application these factors can be controlled and standardized so that the delivery volumes are very reproducible.  With most applications the CV's are less than 5%.

 

1. Pin Diameter
2. Surface tension of the liquid being transferred
3. Surface tension of the pin
4. Speed of removal of pin from source liquid
5. Speed of pin striking recipient dry plate
6. Depth to which the pin is submerged in source plate
7. Depth to which the pin is submerged in recipient plate
8. Volume of slot in pin
9. Surface tension of the dry plate and dwell time

     


  24 Pin E-Clip Style Manual Replicators

     
 


AFIX24FP6

     
 


The AFIX24FP6 was designed to be used in tissue culture "wounding" experiments where a monolayer of tissue culture cells was grown in a 24 well microplate and a V&P floating pin replicator was used to create a precise and reproducible wounding pattern in all the mono-layers. The scientists could then apply different treatments to each well and gauge how effective they were.  The pins in this replicator are separated on 18 mm centers. 

 
 
 


VP 381NW 4.5

 
 
 


  48 Floating Pin E-Clip Style Manual Replicators

     
 


VP 407AHFP13

     
 
3.18 mm diameter pins to deliver ~3 ul hanging drops
     
 


The VP 407AHFP13 is designed to be used by hand.  It comes complete with a BMPM mounting plate and 48 FP13 pins.  The 3.18 mm diameter stainless steel pins are fixtured with an E-Clip to rest on a single float plate.  The pins are on 9 mm center to center spacing.  The tip of the pin has a 3.18 mm diameter flat spot.  As the Replicator is lowered onto the gridding surface each pin rests on the single float plate until it touches on the gridding surface and delivers ~3 ul of liquid in the hanging droplet at the tip.  When the tip hits the surface, the pin floats up through the holes.  This floating action assures that each of the pins touches the surface and will deliver its droplet even if the blotting/gridding surface is not level or smooth.   They are very cost effective and the pins can be easily replaced.  Also it is possible for the customer to purchase slot pins with different dispense volumes and place them into the Replicator to fit assay requirements.  The system is simple enough that the customer can make these changes.

 
 


VP 480FH

     
 


The VP 480FH was designed to be used in tissue culture "wounding" experiments where a monolayer of tissue culture cells was grown in a 48 well microplate and a V&P floating pin replicator was used to create a precise and reproducible wounding pattern in all the mono-layers. The scientists could then apply different treatments to each well and gauge how effective they were.  The pins in this replicator are separated on 13mm centers. 
 

 
 


  96 Floating Pin E-Clip Style Manual Replicators

     
 


1.58 mm diameter pins to deliver ~0.1 ul hanging drops

     
 

The 1.58 mm diameter stainless steel pins are fixtured with an E-Clip to rest on a single float plate.  The other end of the pin is chamfered to a 0.38 mm flat spot on the tip.  As the Replicator is lowered onto the gridding surface each pin rests on the single float plate until it touches on the gridding surface and delivers ~0.1 ul of liquid in the hanging droplet at the tip.  When the tip hits the surface, the pin floats up through the holes.  This floating action assures that each of the pins touches the surface and will deliver its droplet even if the blotting/gridding surface is not level or smooth.  These Replicators are designed to make multiple offset blots to produce moderate density arrays (864 spots/array).  They are very cost effective and the pins can be easily replaced.  Also it is possible for the customer to purchase slot pins with different dispense volumes and place them into the Replicator to fit assay requirements.  The system is simple enough that the customer can make these changes.

     


VP 408FH

     
 


The VP 408FH is designed to be used by hand.  The 1.58 mm diameter stainless steel FP pins are chamfered to a 0 .38 mm flat spot on the tip.  As the Replicator is lowered onto the gridding surface, each pin rests on a single float plate assembly until it touches on the gridding surface and delivers ~0.1 ul of liquid in the hanging droplet at the tip.  When the tip hits the surface the pin floats up through the holes.  This floating action assures that each of the pins will deliver its droplet even if the blotting/gridding surface is not level or smooth.  These Replicators are designed to make nine offset blots to produce moderate density arrays (864 spots/array). 

 

This replicator can also be used to wound tissue culture mono-layers when used in conjunction with a BMPM-PAD and a VP 381NW Library Copier. 

 

96 FP pins, height of Replicator = 48.7 mm, exposed pin length = 23 mm, pin diameter = 1.58 mm, pin tip = 0.38 mm, center to center spacing = 9 mm

 

 
 
 



VP 407FP12

     
 

The VP 407FP12 is designed to be used by hand.  it comes complete with a BMPM and 96 FP12 pins.  The 2.36 mm diameter stainless steel FP12 pins have flat tips.  As the Replicator is lowered onto the gridding surface, each pin rests on a single float plate assembly until it touches on the gridding surface and delivers ~1.5 ul of liquid in the hanging droplet at the tip.  When the tip hits the surface the pin floats up through the holes.  This floating action assures that each of the pins will deliver its droplet even if the blotting/gridding surface is not level or smooth. 

 
 
 


VP 407AFP13

     
 

The VP 407AFP13 is designed to be used by hand.  It comes complete with a BMPM and 96 FP13 pins.  The 3.18 mm diameter stainless steel FP13 pins have flat tips.  As the Replicator is lowered onto the gridding surface, each pin rests on a single float plate assembly until it touches on the gridding surface and delivers ~3 ul of liquid in the hanging droplet at the tip.  When the tip hits the surface the pin floats up through the holes.  This floating action assures that each of the pins will deliver its droplet even if the blotting/gridding surface is not level or smooth. 

A 48 pin version of this pin tool is also available.

 
 
 
 


VP 408AF

     
 


96 deep well floating pin tool using FPAM pins.

 

 

96 FPAM pins, exposed pin length = 52 mm, pin diameter = 1.58 mm, pin tip = 1.0 mm, center to center spacing = 9 mm
 

 

 
 

 
  384 Floating Pin Manual Replicators, E-Clip Style

     
 


1.58 mm diameter pins to deliver ~0.1 ul hanging drops

     
 

The 1.58 mm diameter stainless steel pins are chamfered to a 0 .38 mm flat spot on the tip.  As the Replicator is lowered onto the gridding surface, each pin rests on a single float plate assembly until it touches on the gridding surface and delivers ~0.1 ul of liquid in the hanging droplet at the tip.  When the tip hits the surface the pin floats up through the holes.  This floating action assures that each of the pins will deliver its droplet even if the blotting/gridding surface is not level or smooth.  These Replicators are designed to make two offset blots to produce moderate density arrays (768 spots/array). 

     
 


VP 384F

     
 


384 pins, height of Replicator = 48.7 mm, exposed pin length = 23 mm, pin diameter = 1.58 mm

 

If you want to transfer larger volumes such as a yeast culture to an agar surface we suggest you use our flat tip FP6 pin version of the replicator ....VP 384FP6
 

 


 
 



  Robotic E-Clip Pin Tool

     
 


AFIX384FP12

     
 


The AFIX384FP12 floating fixture can be attached to any of our mounting plates on over 50 robotic work stations.  Solid or slotted pins can be used with this floating fixture.

   
 


AFIX96FP6 with  FPAM pins

     
 


This robotic pin tool was designed to transfer liquid or inoculum from and to deep well microplates or to standard 96 or 384 well or agar Omni Trays.

 

The exposed pin length of the FPAM pins can easily be adjusted by adjusting the height of standoff spacer between the two float plates as illustrated in the photo to the right.

 

 

 


  Microliter Dispensing Pin Tools

     
 


Select The Pin For Your Microliter Dispensing Application
 

     

 
PIN DIAMETER SHAPE
FP 1.58 mm pointed tip
FPS.5 1.58 mm 0.5 ul slot, pointed tip
FPS 1.58 mm 1.0 ul slot, pointed tip
FPS2 1.58 mm 2.0 ul slot, pointed tip
FPS5 1.58 mm 5.0 ul slot, pointed tip
FP6 1.58 mm flat tip
FP6S.5 1.58 mm 0.5 ul slot, flat tip
FP6S 1.58 mm 1.0 ul slot, flat tip
FP6S2 1.58 mm 2.0 ul slot, flat tip
FP6S5 1.58 mm 5.0 ul slot, flat tip
FP13 3.18 mm flat tip
 
 
 
 


The FP, FP6 and the slotted version series slot E-Clip pins are fixtured in a simple float plate and they extend below the plate 23 mm. Slotted pins carry the volume of the slot plus the amount on the sides of the pin and the hanging drop.  So a slot pin will carry slightly more than the slot volume.

     


  Floating Slot Pin Tools, E-Clip

     
 

Slot pins have several advantages over solid pins:

  1. Slot pins carry a larger volume than solid pins in liquid to liquid transfers.

  2. Slot pins are not as sensitive to the depth of the mother liquid as solid pens.

  3. Slot pins deliver a larger volume than solid pins in liquid to dry plate transfers.

  4. Slot pins deliver a larger volume than solid pins in liquid to membrane transfers.

  5. Slot volume is customizable.

  6. Slot pins can deliver to dry plates by pre-wetting the plate. See customer data.

     

 
  Pointed Tip Slot Pins

     
 
VP 408FS.5 Exposed pin length = 23 mm
Pin diameter = 1.58 mm
Slot volume = 0.5 ul
VP 408FS Exposed pin length = 23 mm
Pin diameter = 1.58 mm
Slot volume = 1 ul
VP 408FS2 Exposed pin length = 23 mm
Pin diameter = 1.58 mm
Slot volume = 2 ul
VP 408FS5 Exposed pin length = 23 mm
Pin diameter = 1.58 mm
Slot volume = 5 ul
   


  Flat Tip Slot Pins

     
 

VP 408FP6S.5

Exposed pin length = 23 mm
Pin diameter = 1.58 mm
Slot volume = .5 ul

VP 408FP6S

Exposed pin length = 23 mm
Pin diameter = 1.58 mm
Slot volume = 1 ul

VP 408FP6S2

Exposed pin length = 23 mm
Pin diameter = 1.58 mm
Slot volume = 2 ul

VP 408FP6S5

Exposed pin length = 23 mm
Pin diameter = 1.58 mm
Slot volume = 5 ul
   


  96 Floating Slot Pin Manual Replicator, E-Clip Style

     
 


The 96 stainless steel pins each have a slot cut in the tip of the pin to precisely pick up liquid via capillary action.  Each pin slides in a hole in the 96 hole assembly until it "floats" on the gridding surface and delivers, via capillary action, liquid from the slot at the tip.  This floating action assures that each of the pins will deliver its liquid even if the blotting/gridding surface is not level or smooth.

A significant advantage of the slot pin style is that assures a greater precision in the delivery of the liquid as illustrated in the following table.

For Pin Tool Data And Charts Please Visit This Hyperlink

See this link for data on DMSO and Aqueous delivery with hydrophobic coated and uncoated solid and slot pins
 

 


 
 



  384 Floating Slot Pin Manual Replicator E-Clip Style

     
 


The 384 stainless steel pins have a slot cut in the tip of the pin to precisely pick up liquid, via capillary action.  Each pin slides in a hole in the 384 hole assembly until it "floats" on the gridding surface and delivers via capillary action, liquid from the slot at the tip.  This floating action assures that each of the pins will deliver its liquid even if the blotting/gridding surface is not level or smooth.

 
 
 


FP6S Slot Pins - Flat Tip

VP 384FP6S.5 Flat tip
Exposed pin length = 22 mm
Pin diameter = 1.58 mm
Slot volume = 0.5 ul
VP 384FP6S Flat tip
Exposed pin length = 22 mm
Pin diameter = 1.58 mm
Slot volume = 1 ul
VP 384FP6S2 Flat tip
Exposed pin length = 22 mm
Pin diameter = 1.58 mm
Slot volume = 2 ul
VP 384FP6S5 Flat tip
Exposed pin length = 22 mm
Pin diameter = 1.58 mm
Slot volume = 5 ul
   


FP Slot Pins - Pointed Tip

VP 384FS.5 Pointed tip
Exposed pin length = 23 mm
Pin diameter = 1.58 mm
Slot volume = 0.5 ul
VP 384FS Pointed tip
Exposed pin length = 23 mm
Pin diameter = 1.58 mm
Slot volume = 1 ul
VP 384FS2 Pointed tip
Exposed pin length = 23 mm
Pin diameter = 1.58 mm
Slot volume = 2 ul
VP 384FS5 Pointed tip
Exposed pin length = 23 mm
Pin diameter = 1.58 mm
Slot volume = 5 ul
     


  Manual E-Clip Style Replicator Accessories

     
 


We suggest you buy a few inexpensive accessories that will make the Replicators more useful, easier, safer and more reproducible. 

The VP 381 is a Registration Device that aligns the Replicator pins to the center of the wells.  This is very important as you do not want the pins to touch the side of wells as you remove the pins because that will knock off the hanging drop at the pin tip.  The VP 381 LIBRARY COPIER™ is also used with our 96 pin Replicators to re-array from four, 96 well plates to one, 384 well plate or vice versa, to break out samples from one, 384 well plate to four, 96 well plates.

The VP 382 MULTI-PRINT™ is a registration device that allows you to make membrane arrays in an area the size of a standard microtiter plate with our Replicators.  Either four, 384 well plates or nine, 96 well plates can be arrayed into a 1536 or 864 array on the membrane.

After arraying from four, 384 well plates to one, 1536 membrane array (or nine, 96 well plates to one, 864 membrane array), the VP 430 Reading Mat helps locate which mother plate well produced a positive spot on the membrane array.

The VP 420 is a Glass Alcohol Reservoir that is used in the sterilization of the Replicators by flaming.  Some labs use plastic reservoirs, but if flaming alcohol drops into them the plastic burns, dumping flaming alcohol all over the counter.  This covered dish is also useful for storing the alcohol as well as VP 110 Pin Cleaning Solution (below) for reuse.  

The VP 425 is a Pin Cleaning Brush that is used to keep the Replicator pins clean so they will carry the same amount of liquid each time.   

Our VP 110 is a Pin Cleaning Solution that is used to clean and treat the pin tips at the beginning of the day.  

Integral to the cleaning process of all pins is the blotting step between baths.  Lint particles in the slots or pin tips, from blotting onto standard paper towels, will interfere with the loading and unloading of liquid.  The VP 522 is a Lint Free Blotting Paper that doesn't transfer lint particles to the pins as many blotting papers or paper towels can do.  We strongly recommend that you use these blotting pads placed over several layers of paper towels or our super absorbent VP 540DB polypropylene pad/tray system.

These accessories are also found in the Basic Membrane Array Starter Kit VP OK14A at a 5% discount.

 


  Manual Membrane Macro Array Starter Kit Accessories for E-Clip Style

 
 


For your convenience and a 5% discount, V&P Scientific has combined the essential components of our Floating E-Clip Style 96 and 384 Pin MULTI-BLOT™ Replication System into a Starter Kit to make up to 4 identical 864 or 1536 feature Macro Arrays at once.  You select whichever Solid or Slot Pin E-Clip Style MULTI-BLOT™ Replicator that fits your application and delivery volume,  then just add it to the Basic Macro Array Starter Kit VP OK14 and you have everything you need to start making arrays.  Included in the Starter Kit are the following: 
 

     
 

VP OK14A

Four, MULTI-PRINTS VP 382
One, LIBRARY COPIER™ VP 381N
One, V&P Pin Cleaning Solution Bottle VP 110
One, Package Of Lint Free Blotting Paper VP 522
One, Pyrex Alcohol Reservoir With Lid VP 420
One, Clear Overlay Reading Mat For 864 And 1536 Arrays VP 430
Five, Plastic Bleach Or Water Reservoirs VP 421
One, Pin Cleaning Brush VP 425
One, 30 cm by 3 meter Roll Of Hybond N Nylon Membrane VP 503
 


  Defining a Pin Tool

     
 


Originally we sold our pin tools with unique part numbers.  As we have added more pins to our selection and more robot systems to mount the pin tools to, the number of unique part numbers grew exponentially to an un-manageable size.  Our solution has been to specify a pin tool by the 3 component parts that compromise the Pin Tool.  The Pins, the Floating Fixture and the Robot Mounting Plate.  If you have previously ordered a pin tool by unique part number, we will be able to provide the same pin tool to you.

     


  Ordering a Robot E-Clip Pin Tool Is As Simple as 1,2,3

     
 
An E-Clip robot pin tool consists of three separate parts.

1. The pins, (#FP, FP6 or slotted versions)
2. The Floating Frame Fixture (96 or 384) (#AFIX___FP6_)
3. The Basic mounting plate that attaches the Floating Frame Fixture to the robot (#BMP_______) (what robot are you using?)

For example the part numbers for pin tool in the photos to the right would be FP6S5, AFIX96FP6 and BMPMULTIMEK.

To order, just tell us those 3 part numbers and we will assemble and price your Pin Tool.

Determine the weight of a Robot pin tool at this link

 


 
 


The BGPKL kit is used when longer replicator pins are used like the FPAM pins which are 63 mm long.   A pin tool with FPAM pins is illustrated in the photo to the right.   In order to accommodate these longer pins we need to use a longer and more sturdy guide pin found on the BGPL frame.  These larger guide pins are 3.17 mm in diameter.

Each of the kits on the right (BGPKL and BGPK) is made up of a handle, a manual mounting plate and a guide pin frame.  The guide pin frame is the only  difference between the two kits

     
 


NEW
Many of our customers who are working with inoculating yeast on agar plates (Omni Trays) wanted a pin tool that would not only deliver a larger volume (~3 ul) but would also float, so if the agar surface was not perfectly level, the liquid would still be transferred.  This necessitated the development of a larger diameter (3.175 mm) pin and putting an e-clip on it so it can float.  With this pin (FP13)  we only need one floating plate (AFIX96FP13).   This pin tool can also be provided in a manual version.

 
 
 
 
 
 
         

 
  Conversion Kit To Change E-Clip Pin Tools To Multi-Blot Replicators

 
 


Conversion of robot pin tools to manual Multi-Blot Replicators is very easy using our BGPK for Standard length pins and the BGPKL for longer pins.  Simply remove the robot mounting feature from the float plates disassemble the pin tool and reassemble it with the Guide Pin Frame as part of the tool.  Attach the Manual Mounting Plate and handle and you are ready to go.   If this sounds too daunting for you - send it back to us and we will do it for you for a nominal fee.

The main reason for this conversion is to attach  guide pins to the replicator so it can be aligned to Microplate registration accessories such as the VP 381 series Library copiers for replicating and reformatting genomic libraries, VP 380 series colony copiers to make high density arrays on agar surfaces or  VP 382 membrane printers to make high density arrays on nylon or other membranes.

The BGPKL kit is used when longer replicator pins are used like the FPAM pins which are 63 mm long.   A pin tool with FPAM pins is illustrated in the photo to the right.   In order to accommodate these longer pins we need to use a longer and more sturdy guide pin found on the BGPL frame.  These larger guide pins are 3.17 mm in diameter.

Each of the kits on the right (BGPKL and BGPK) is made up of a handle, a manual mounting plate and a guide pin frame.  The guide pin frame is the only  difference between the two kits

Some of our customers wanted to transfer organisms from a deep well microplate to an agar Omni Tray (VP 413).  Using our 45 mm exposed length FPAM pins we can reach the bottom of all conventional deep well microplates and still float lightly on an uneven agar surface to make sure that every pin contacts the surface.  The floating pin system assures that every pin contacts the agar surface and the inoculum is transferred uniformly.

We are able to use the BGPKL kit and interchangeably use this pin tool with robots and/or manually. 

 


 
 


The manual version of the pin tool can be used with both deep well and standard 96 microplates as well as standard 384 microplates as shown to the right and below.

 
 
 


Using our VP 381B microplate registration and alignment tool we can reformat libraries from 96 to 384 or visa versa.

 
 


  Robot Pin Tool Accessory Kit

     
 
We highly recommend that you order the Robot Pin Tool Accessory kit at the time you order the pin tool.  This "essentials" accessory kit contains the VP 540DB Lint Free Blotting System used to clean the pins efficiently between samples, two VP 540 Wash Reservoirs to wash the pins between source plates, the VP 110 Pin Cleaning Solution is used the clean the pins daily, the VP 425 Pin Cleaning Brush is used if a build up occurs and the VP 555 Safe Pin Tool Storage System is used to keep it clean and the fragile pins out of harms way.   Although we have several other wash and blot accessories available, these are the most cost effective for the majority of applications.  If you elect to use the fountain and flowing reservoirs we still strongly recommend the other items in this kit.  When purchased as the VP OK17 Kit you will receive a 5% discount over the sum of the individual items
 
 
 

Robot Pin Tool Accessory Kit - VP OK17

Lint Free Blotting System with pad (1) VP 540DB
Pin Wash Reservoirs (2) VP 540
Pin Cleaning Solution (1) VP 110
Pin Cleaning Brush (1) VP 425
Safe Pin Tool Storage Station (1) VP 555
     
 


Click here for a list of published articles that cite using the products on this page.

 
         

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