We have developed a fail safe colony
picking/arraying system for 48, 96 and 384 well microplates. It
also provides a sterile cover to prevent airborne contamination
while you are filling the plate. Just place the Toothpick holder
over the plate and inoculate the wells by placing wood (VP 371)
or plastic (VP 372) toothpicks through the holes in the Toothpick
holder. Leave the toothpicks in the holder to mark your place
until you finish the whole microplate, then lift the holder with
the toothpicks off the microplate, invert and discard the
toothpicks. Autoclave or clean the Toothpick Holder with bleach
and or alcohol and reuse.
The wood Toothpicks (VP 371) can be sterilized by Autoclaving and the
plastic Toothpicks (VP 372) can be sterilized with ethylene oxide, Gamma
radiation or UV light. Many of our customers don’t sterilize the toothpicks as
they are already very clean and/or they are working in systems that use
antibiotic resistance strains and they culture in media that contains that
The Toothpick holders are also useful if you
are re-arraying a library to place all the positives on one
plate. For even faster colony picking or re-arraying check out the new
V&P Colony Picker VP 373.
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