Colony Copier

     
 


The COLONY COPIER™ is a simple registration accessory for 48, 96 and 384 pin MULTI-BLOT™ Replicators. The COLONY COPIER™ is placed over a Nunc Omni Tray and aligns itself to the external surfaces of the Omni Tray and precisely registers the MULTI-BLOT™ Replicator's stainless steel pins to the interior of the Omni Tray. High density offset arrays of blots or colonies are produced on a single Omni Tray by inoculation at each alignment hole pair using our unique system of guide pins and alignment hole pairs.


Click here for a list of published articles that cite using the products on this page.

 

 


  Inoculate 48 to 1536 Cultures on a Single Agar Plate:

 
   
  • Antibiotic sensitivity assays
  • Drug sensitivity assays
  • Growth assays
  • Plaque assays
 


  Screen High Density Grids of Cultures, Genomic DNA or cDNA Libraries on Precut    Membranes:

 
   
  • Cosmids

  • Mutants

  • Plasmids

  • Recombinant clones

  • YACs

 


  Construct & Screen High Density Arrays of:

     
   
  • cDNA

  • RNA

  • Antibodies

  • Drugs

  • PCR products

  • DNA

  • Antibiotics

  • Antigens

  • Ligands

 

 
 
 


The COLONY COPIER™ (VP 380) has 2 sets of 9 alignment holes on the front frame (a 3 by 3 array for 48, 96 pin MULTI-BLOT™ Replicators yielding 432 and 864 blots) and 2 sets of 4 alignment holes on the rear frame (a 2 by 2 array for 384 pin MULTI-BLOT™ Replicators yielding 1,536 blots).  The high density COLONY COPIER™ (VP 380D) has 2 sets of the alignment holes on the front frame (a 4 x 4 array for 96 pin MULTI-BLOT™ Replicators yielding 1536 blots and 768 blots for 48 pin MULTI-BLOT™ Replicators). If you are transferring DNA, cDNA or other materials in hydrophobic solutions you will find that our new V&P Pin Cleaning Solution treatment of the pins will greatly increase the precision of delivery.  For an explanation see the FAQ page and the V&P Pin Cleaning Solution page.


Click here for a list of published articles that cite using the products on this page.

 
 
 


 
 
 


  Transfer Ordered Libraries or Substances to Membranes for Assay:

 
   
  • Avidin-biotin
  • Hybrid selection
  • Ligand binding
  • Hybridization
  • Immunoassay
  • Receptor binding
       
 
 


Microbial cultures can also be inoculated and grown upon a variety of membranes (VP 504-508) notched to fit in the Omni Tray and placed on the surface of nutrient agar, agarose or on a notched wicking paper containing nutrient broth. This latter method is used when material from the agar results in high non-specific background binding to the membrane.

 

High density blots of substances on notched membranes in Omni Trays are amendable to a wide variety of assay methods. In addition the Omni Tray can be used as an incubation chamber to process the assay.

     


  Accommodates Wide Variety of Detection Methods:

     
   
  • Chemiluminescent
  • Chromogenic (enzyme based)
  • Fluorescent
  • Isotopic
 
 


Libraries of cDNA expression, antigens, antibodies or other substances can be blotted on a membrane in the bottom of an empty Omni Tray and the Omni Tray used to process the membrane for a variety of screening assays including hybridization, ligand binding, antigen or antibody detection.

     


  Store Working Culture Libraries on Agar

     
   

Culture libraries can be inoculated onto agar Omni Trays, grown into colonies and stored in the refrigerator for 7 to 8 weeks. In this format the colonies are used as a working reference library, and the master library is spared frequent freeze/thaw cycles that may decrease culture viability and increase the risk of contamination. You can access the colony library by simply using the replicator to "pick" the colonies and transfer them to your test media or solid substrate. Usually there is enough colony transferred that you do not have to wait for the cultures to grow.

     


  Method Used with Pin Tools and Colony Copiers to
  M
ake Synthetic Genetic Arrays

 
 


Article can be viewed here.

     


  Pick Clones from Screening Plates with a 384 Pin Replicator

     
   

Whole libraries can also be screened in mass and colonies or plaques "picked" in mass from Omni Trays with the 384 pin MULTI-BLOT™ Replicator registered to a COLONY COPIER™. This procedure is far more efficient and superior to individually picking colonies or plaques with tooth picks. We recommend the VP 384 Replicator because of its larger pin size.

     


  1536 Colonies

     
 


High Density Array (1536 Colonies) of Pigmented Bacteria on Omni Tray

 
 

 
  VP 380 -
COLONY COPIER

     
   

FEATURE

BENEFIT

Precision Machined to Fit Around Omni Tray
  • Registers MULTI-BLOT™ Replicators to the Omni Tray.

Two Alignment Hole Patterns for Replicator Guide Pins:

  • Provides registration system for Replicator pins on MULTI-BLOT™ Replicators to an Omni Tray.
     

  • Provides registration system for the mass picking of recombinant clones or colonies in a working library.

*Two sets of 9 holes on front frame

*Registers 96 pin MULTI-BLOT™ Replicators to the Omni Tray for high density arrays (9 x 96 = 864).

*Registers 48 pin MULTI-BLOT™ Replicators to the Omni Tray for high density arrays (9 x 48 = 432).

 

Two sets of 4 holes on rear frame. Registers 384 pin MULTI-BLOT™ Replicators to the Omni Tray for high density arrays (4 x 384 = 1536).
 
   
COLONY COPIER™, registers Omni Tray VP 380
COLONY COPIER™, 4 x 4 Array for 96 Pin Replicators VP 380D
Nunc Omni Tray, sterile with lid VP 413
Reading Mats for 96 and 384 High Density Arrays VP 430
 
         


Pin Tool Transfer to Agar Plates

     
 


Control - VP 407AM Pin Tool transferring Bacteria

 
 
 


Control - VP 408A Pin Tool transferring Bacteria

 
 
 


Condition 3 - VP 407AM Pin Tool transferring Bacteria

 
 
 


Condition 3 - VP 408A Pin Tool transferring Bacteria

 
 
 


B-407A Pin Tool Transferring Yeast (Saccharomyces cerevisiae)

 
 
 


B-409 Pin Tool Transferring Yeast (Saccharomyces cerevisiae)

 
 
 


FP6 Pin Tool Transferring Yeast (Saccharomyces cerevisiae)

 
 
 


FP6 Dilution Experiment - Pin Tool Transferring Yeast (Saccharomyces cerevisiae)

 
 
 


FP12 Concentrated Yeast Experiment Pin Tool Transferring Yeast (Saccharomyces cerevisiae)

 
 
 


FP Pin Tool Concentrated Yeast Pin Tool Transferring Yeast (Saccharomyces cerevisiae)

 
 
 


FP Pin Tool dilute yeast - Pin Tool Transferring Yeast (Saccharomyces cerevisiae)

 
 
 


Click here for a list of published articles that cite using the products on this page.

 
         

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