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The new V&P Scientific, Inc. DNA Card Registration Tool and Slot Pin Spotter provides a simple
archiving solution to all those laboratories that are looking to
transfer 96 specimens, 5 ul at a time to the revolutionary new CloneSaver 96 Card*. This new innovation in archiving DNA libraries is a revolution
in the way genomic material is stored. No longer do you have to take up
valuable freezer space with frozen specimens or glycerol stocks in 96 well
microplates, just spot them on the CloneSaver 96 Card with the 5 ul
Slot Pin Spotter (VP 408S5) and the DNA Card Registration Tool (VP
382CS).
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Whether you use the spotter on fresh stocks or on
previously frozen specimens here is an extremely efficient and simple means to
accurately place your DNA into a safe and convenient storage system. If
your lab is being crowded out with ultra low temperature freezers filled with genomic stocks, here is a very simple way to recover freezer space.
Simply thaw those genomic stocks and transfer them to CloneSaver 96 Card
using the Slot Pin Spotter (VP 408S5) and the DNA Card Registration Tool (VP 382CS). Just slide the CloneSaver
Card into the slot on the
DNA Card
Registration Tool (VP 382CS) and position the 4 smaller circles on the
CloneSaver Card so they are inside the 4 half circles on the DNA Card
Registration Tool. Pick up exactly 5 ul of specimen by simply placing the
slot pins of the Slot Pin Spotter into a 96 well microplate.
These specially tooled pins pick up precisely 5 ul by capillary action in the
slot. When the guide pins of the Slot Pin Spotter are placed in the alignment holes
of the DNA Card Registration Tool, the slot pins are registered to the centers of the 96
large circles on CloneSaver Card. When the slot pins touch the
surface of the CloneSaver Card, all the liquid in the slot wicks into the
paper by capillary action. It is as simple as 1, 2, 3. The liquid stays
inside the circle lines and you can monitor the spot size by the color change on
the CloneSaver Card.
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The V&P Slot Pin Spotter is made with stainless slot pins pressed into an anodized
aluminum base. Its rugged construction allows it to be sterilized by
autoclaving, heating in a hot air oven, alcohol flaming or dipping in
bleach. It can also be disinfected by soaking in H2O2 and cleaned in
ultrasonic baths. For most applications cleaning the V&P Slot Pin Spotter
between microplates is rapidly accomplished by dipping for 5 seconds in a 5%
bleach solution, followed by 3 dips in distilled water baths followed by an ethanol bath and
flaming. The whole cleaning procedure takes only 20 to 30
seconds.
The VP 382CS DNA Card Registration Tool is also constructed using anodized aluminum
and stainless steel tension springs. It can also be autoclaved or cleaned
with bleach/ethanol.
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Integral to the cleaning process is the blotting step between baths. We
have found an inexpensive lint free blotting paper
(VP 522) to use that doesn't transfer lint
particles to the slot pins as many blotting papers or paper towels can do. Lint
in the slots can interfere with the quantitative transfer of liquid. We strongly recommend
that you use these blotting pads over several layers of paper towels.
Our MULTI-BLOT™ Slot
Pin Replicators are provided with various slot sizes. The photo below
indicates our standard 0.5, 1, 2 and 5 ul slot pins plus a custom 3.2
ul
slot. If you need a custom volume delivered just contact us.
*Manufactured by the Whatman Group.
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