Stir Element Data

     
 


Ability of VP 710S-2 and VP 710C1 Alligator Vertical Tumble Stirrers to stir microplates*** stacked on the deck and each plate filled with different Stir Elements.  Deck position, type of Stir Element and how high the plates could be stacked and still stir were determined.

 
 


Methods:

     
 


Alligator Vertical Tumble Stirrers were set to 100 RPM, microplates were filled with 200 ul of water, deep well blocks were filled with 1.5 ml of water, one stir element was added to each well.  In order to be counted as positive stirring all the stir elements in the plate had to exhibit vertical tumbling or in the case of Stir StiXs back and forth movement. In order to error on the side of under claiming we subtracted 2 microplates (28 mm in stirring height) from each of the totals on microplates and 1 plate (42 mm in stirring height) from each of the totals on the deep well blocks.  These are thus very conservative estimations.

The data shows that the relative position of the plate to magnetic cylinder is important to the height that plates can be stacked and still be stirred.

The deck of VP 710S-2, magnetic cylinder runs directly under positions 1, 2 and 3.

 
 
 
 
  PLATE POSITION 1 2 3 4 5 6 7  
Stirrer Stirring Element Max Stack Plates Max Stack Plates Max Stack Plates Max Stack Plates Max Stack Plates Max Stack Plates Max Stack Plates

Total Plates

VP 710S2* VP 711 13 15 11 12 11 12 12 86
VP 710S2* VP 711-1 17 19 14 12 12 13 12 99
VP 710S2* VP 711D 16 21 18 13 14 17 16 115
VP 710S2* VP 711D-1 17 21 17 15 16 17 16 119
VP 710S2* VP 711D-3 18 21 19 14 17 18 17 124
VP 710S2* VP 712 16 18 19 17 17 15 14 116
VP 710S2* VP 712-1 10 12 10 9 10 9 11 71
VP 710S2* VP 712-3 15 16 14 16 13 15 16 105
VP 710S2* VP 712-2 14 15 15 7 7 9 10 77
VP 710S2* VP 712D 9 8 9 8 10 7 6 57
VP 710S2* VP 716 16 16 16 13 15 14 14 104
VP 710S2* VP 716-1 12 13 9 8 7 10 10 69
VP 710S2* VP 717 32 36 34 29 32 35 35 233
VP 710S2* VP 717-1 30 29 26 26 17 24 20 172
VP 710S2* VP 719 5 13 4 3 0 9 9 43
VP 710S2* VP 719-1 4 8 3 0 0 4 1 20
VP 710S2* VP 719F 9 14 7 3 5 7 8 53
VP 710S2* VP 719F-1 8 11 7 4 4 7 7 48
VP 710S2* VP 719F-2 6 7 4 0 0 4 4 25
VP 710S2* VP 720 9 9 6 0 0 2 2 28
VP 710S2* VP 720F 8 8 7 0 0 3 2 28
VP 710S2* VP 720F-1 8 9 6 0 0 5 3 31
VP 710S2* VP 721-1 5 9 5 0 0 5 5 29
VP 710S2* VP 721F 6 13 5 6 9 9 9 57
VP 710S2* VP 721F-1 9 15 13 3 5 7 7 59
VP 710S2* VP 722 8 10 8 2 0 4 7 39
VP 710S2* VP 722F 4 10 5 4 4 5 6 38
VP 710S2* VP 722F-1 8 16 10 4 4 7 9 58
VP 710S2* VP 722F-2 3 6 4 5 4 3 1 26
VP 710S2** VP 733 12 14 11 7 7 11 11 73
VP 710S2** VP 734 12 14 11 8 7 11 11 74
VP 710S2** VP 735 12 15 11 8 7 11 9 73
VP 710S2** VP 733-2 13 17 12 8 8 12 11 81
VP 710S2** VP 734-2 13 16 12 7 8 11 11 78
VP 710S2** VP 735-2 13 16 12 7 7 12 11 78
 
     


Landscape Position


Portrait Position

 
 
 
  Plate Position 1 2 3 2 & 3
Stirrer Stirring Element Max Stack Plates Max Stack Plates Max Stack Plates Total Plates
VP 710C-1* VP 711 8 6 5 11
VP 710C-1* VP 711-1 8 6 6 12
VP 710C-1* VP 711D 8 6 6 12
VP 710C-1* VP 711D-1 7 5 5 10
VP 710C-1* VP 711D-3 9 7 6 13
VP 710C-1* VP 712 9 8 8 16
VP 710C-1* VP 712-1 7 6 5 11
VP 710C-1* VP 712-3 7 5 5 10
VP 710C-1* VP 712-2 1 0 0 (1)
VP 710C-1* VP 712D 6 6 5 11
VP 710C-1* VP 716 8 7 7 14
VP 710C-1* VP 716-1 5 3 1 (5)
VP 710C-1* VP 717 15 14 14 28
VP 710C-1* VP 717-1 15 13 13 26
VP 710C-1* VP 719 6 1 1 (6)
VP 710C-1* VP 719-1 3 0 0 (3)
VP 710C-1* VP 719F 7 2 2 (7)
VP 710C-1* VP 719F-1 5 1 1 (5)
VP 710C-1* VP 719F-2 2 0 0 (2)
VP 710C-1* VP 720 3 0 0 (3)
VP 710C-1* VP 720F 3 1 1 (3)
VP 710C-1* VP 720F-1 3 1 1 (3)
VP 710C-1* VP 721-1 2 0 0 (2)
VP 710C-1* VP 721F 6 2 2 (6)
VP 710C-1* VP 721F-1 4 3 3 6
VP 710C-1* VP 722 4 0 0 (4)
VP 710C-1* VP 722F 2 0 0 (2)
VP 710C-1* VP 722F-1 5 2 2 (5)
VP 710C-1* VP 722F-2 2 0 0 (2)
VP 710C-1* VP 724F 5 2 4 6
VP 710C-1* VP 724F-1 4 1 1 (4)
VP 710C-1** VP 733 5 3 3 6
VP 710C-1** VP 734 5 3 3 6
VP 710C-1** VP 735 4 3 3 6
VP 710C-1** VP 733-2 5 3 3 6
VP 710C-1** VP 734-2 4 2 2 4
VP 710C-1** VP 735-2 4 2 2 4
 
 
*Testing performed using standard Greiner 96 well polystyrene plates (14 mm tall) with 200 ul of water

** Testing performed using Deep well AB gene polypropylene plates (42 mm tall) with 1.5 ml of water.

() Indicates that position 1 was superior to the sum of positions 2 and 3.

     
 


Ability of VP 708 Vortex Lateral Tumble Stirrer to stir microplates stacked vertically around the magnetic cylinder  and each plate filled with different Stir Elements.  Vertical position (top of cylinder, middle of cylinder and bottom of cylinder,) were tested for their ability to spin the various stir elements.

 
 


Methods:

     
 


Vortex Lateral Tumble Stirrer was initial set to the lowest speed of 34 RPM.  One stir element was added to each well of the microplate.  Testing included: increasing to the maximum speed of1462 RPM, observing all wells for proper stirring, and placing the microplate in 3 different position of the stirrer.  In order to be counted as positive stirring all the stir elements in the plate had to exhibit vortex tumbling movement.  These are very conservative estimations.

The data shows that the relative position of the plate on the Vortex Lateral Tumble Stirrer is important to where certain stir elements will stir.  Plate position on the stirrer depended on where the stir element uses deep well microplates or standard well microplates.

     
 


Standard Plate Configuration
Stainless Steel Stir Elements

     
   
  Plate Position
Part Top
1
Middle
4
Bottom
7
 VP 711 yes yes yes
 VP 711-1 yes yes yes
 VP 711D yes yes no
 VP 711D-1 yes yes yes
 VP 711D-3 yes yes yes
 VP 712 no yes yes
 VP 712-1 no yes no
 VP 712-2 no no no
 VP 712-3 yes yes no
 VP 716 yes yes yes
 VP 716-1 yes yes yes
 VP 717 yes yes yes
 VP 717-1 yes yes yes
 
 
 


Deep Well Plate Configuration
Stainless Steel Stir Elements

     
   
  Plate Position
Part Top
1
Middle
2
Bottom
3
 VP 733 yes yes yes
 VP 733-2 no yes no
 VP 734 yes yes yes
 VP 734-2 no yes no
 VP 735 yes yes yes
 VP 735-2 no no no
 VP 736 yes yes yes
     
 
Magnetic Stir Elements
     
   
  Plate Position
Part Top
1
Middle
2
Bottom
3
 VP 774-2 yes yes yes
 VP 774-5 yes yes yes
 VP 774-7 yes yes yes
 VP 774-8 yes yes yes
 VP 774-15 yes yes yes
 VP 775-3 yes yes yes
 VP 775-6 yes yes yes
 VP 775-8 yes yes yes
 VP 775-10 yes yes yes
 VP 775-12 yes yes yes
 VP 775-13 yes yes yes
 VP 775-20 yes yes yes
 VP 775-30 yes yes yes
 VP 775-35 yes yes yes
 VP 776-25 yes yes yes
 VP 776-40 yes yes yes
 VP 776-50 yes yes yes
 VP 777-30 yes yes yes
 VP 777-38 yes yes yes
 VP 777-40 yes yes yes
 VP 777-51 yes yes yes
 VP 777OV-64 yes yes yes
 VP 778-12 yes yes yes
 VP 778-25 yes yes yes
 VP 778-35 yes yes yes
 VP 778-50 yes yes yes
 VP 779-5 yes yes yes
 VP 779-6 yes yes yes
 VP 779-13 yes yes yes
 
 
         

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