Effect of DNA* or BSA# concentration on Slot Pin transfer's

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Effect of DNA* or BSA^# concentration on Slot Pin transfer's of uncoated and
Hydrophobic/Lipophobic Coated Pins

LIQUID-TO-LIQUID TRANSFERS
Solvent/Sample	Conc.	FP3CS500 Slot Pins
		Uncoated		Hydrophobic Coated
		CV%	nl FITC Transferred	CV%	nl FITC Transferred
DMSO (-)	0	8.1%	353.42	7.5%	298.72
*DMSO + DNA (mg/ml)**	0.5	6.6%	497.21	6.6%	435.86
	0.25	9.0%	432.49	4.1%	391.93
	0.125	8.9%	363.64	0.9%	344.75
	0.0625	2.3%	381.86	2.0%	331.68
	0.0313	1.5%	378.03	4.4%	331.71
	0.0156	1.2%	357.52	1.4%	329.03
Tris (-)	0	4.9%	577.31	7.2%	493.53
Tris + DNA (mg/ml)
	0.5	4.5%	540.53	1.1%	477.50
	0.25	4.6%	518.21	6.1%	456.75
	0.125	15.8%	583.25	4.1%	438.82
	0.0625	4.2%	551.17	3.1%	433.69
	0.0313	4.4%	536.66	2.3%	458.37
	0.0156	2.9%	528.53	1.2%	441.10
Tris + BSA (%)	4	5.4%	462.13	11.0%	409.27
	1	4.0%	452.86	2.7%	426.58
	0.25	11.7%	456.45	1.3%	408.72
	0.0625	1.1%	445.22	6.5%	393.07
	0.0156	3.7%	462.85	3.9%	430.20
	0.0039	1.5%	493.54	2.2%	437.29
	0.0010	2.9%	504.25	0.7%	475.96

* = sheared salmon sperm DNA

# = Bovine Serum Albumin

General Observations:

Uncoated and hydrophobic coated FP3S500 Slot Pins

1. Increasing the concentration of DNA to .25 mg/ml significantly increases the volume of DMSO liquid transferred for both coated and uncoated FP3S500 Slot Pins.

2. Increasing the concentration of DNA does not significantly increase the volume of Tris buffer (aqueous) transferred by both coated and uncoated FP3S500 Slot Pins.

3.Increasing the concentration of BSA significantly decreases the volume of Tris buffer transferred by both coated and uncoated FP3S500 Slot Pins.

4. Hydrophobic coated FP3S500 Slot Pins transferred less DMSO - DNA and less Tris DNA and less Tris BSA than the uncoated FP3S500 Slot Pins.

5. Both coated and uncoated FP3S500 pins transfer significantly more aqueous solution than DMSO.

Effect of DNA concentration on Slot Pin transfer's of uncoated and
Hydrophobic/Lipophobic Coated Pins

LIQUID-TO-LIQUID TRANSFERS
Solvent/Sample	Conc.	FP1CS50 Slot Pins
		Uncoated		Coated
		CV%	nl FITC Transferred	CV%	nl FITC Transferred
DMSO (-)	0	4.2%	49.38	2.1%	49.31
DMSO + DNA (mg/ml)	0.5	4.9%	51.24	2.6%	56.79
	0.25	1.7%	50.20	1.2%	49.53
	0.125	1.5%	51.27	2.3%	49.77
	0.0625	2.2%	49.34	4.1%	48.19
	0.0313	1.2%	49.03	0.2%	50.23
	0.0156	2.4%	45.90	1.4%	46.64
Tris (-)	0	2.6%	89.51	2.9%	91.34
Tris + DNA (mg/ml)	0.5	7.0%	77.11	0.6%	84.62
	0.25	3.9%	82.22	1.6%	84.89
	0.125	3.9%	85.42	1.0%	85.08
	0.0625	1.5%	85.36	2.8%	85.03
	0.0313	2.0%	84.52	3.0%	88.19
	0.0156	2.6%	82.92	2.8%	83.20

General Observations:

Uncoated and hydrophobic coated FP1S50 Slot Pins

1. In contrast to the FP3S500 data, increasing the concentration of DNA to .25 mg/ml does not significantly increase the volume of DMSO liquid transferred for both coated and uncoated FP1S50 Slot Pins.

2. Increasing the concentration of DNA does not significantly increase the volume of Tris buffer (aqueous) transferred by both coated and uncoated FP1S50 Slot Pins.

3. In contrast to the FP3S500 data, FP1S50 coated pins transferred about same volume of DNA at all concentrations as did uncoated pins.

4. Both coated and uncoated FP1S50 pins transfer significantly more aqueous solution than DMSO.

5. The differences between the FP3S500 and the FP1S50 pin maybe due to the different pin diameter's affect on contact angle and therefore on the "wetting" of the pin. See diagrams on hydrophobic action.