Effect of DNA* or BSA# concentration on Slot Pin touch off transfer's

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Effect of DNA* or BSA^# concentration on Slot Pin touch off transfer's of
uncoated and Hydrophobic/Lipophobic Coated Pins on Polystyrene plates
(08-02-02)

DRY PLATE TOUCH-OFF (LIQUID-TO-DRY PLATE)
Sample	Conc.	FP3CS500 Slot Pins
		Uncoated		Hydrophobic-Coated
		CV%	nl FITC Transterred	CV%	nl FITC Transterred
DMSO (-)	0	42.8%	9.39	20.5%	7.43
DMSO + DNA (mg/ml)
	0.5	16.0%	5.47	24.2%	5.81
	0.25	66.1%	7.01	9.3%	7.20
	0.125	15.4%	5.25	11.8%	8.23
	0.0625	56.0%	8.30	31.1%	6.73
	0.0313	32.2%	6.91	10.4%	8.13
	0.0156	10.6%	7.93	30.2%	5.64
Tris (-)	0	9.8%	37.22	21.3%	33.84
Tris + DNA (mg/ml)
	0.5	4.9%	38.17	25.8%	31.59
	0.25	3.4%	39.82	69.9%	27.84
	0.125	8.0%	40.35	17.2%	32.34
	0.0625	6.8%	36.72	13.0%	33.16
	0.0313	3.6%	38.31	8.6%	34.93
	0.0156	14.6%	35.30	14.5%	34.96
Tris + BSA (%)	4	9.5%	30.81	67.5%	22.47
	1	4.9%	32.09	4.2%	29.87
	0.25	14.4%	32.10	8.1%	28.67
	0.0625	6.0%	34.21	2.3%	30.76
	0.0156	6.1%	35.38	6.5%	31.47
	0.0039	4.5%	34.54	18.5%	29.64
	0.0010	9.9%	40.94	15.5%	34.70

* = sheared salmon sperm DNA

# = Bovine Serum Albumin

GENERAL OBSERVATIONS

1. Dry touch off transfers with DNA in DMSO are not very reproducible with either coated or uncoated pd for both coated FP3S500 Slot Pins. This may be due to small percentage of liquid transferred.

2. Increasing the concentration of DNA did not significantly affect the volume of Tris buffer (aqueous) transferred by either coated and uncoated FP3S500 Slot Pins.

3.Increasing the concentration of BSA significantly decreases the volume of Tris buffer transferred by both coated and uncoated FP3S500 Slot Pins.

4. Hydrophobic coated FP3S500 Slot Pins transferred slightly less DMSO - DNA and less Tris DNA and less Tris BSA than the uncoated FP3S500 Slot Pins.

5. Both coated and uncoated FP3S500 pins transfer significantly more aqueous solution than DMSO.

6. Coated FP3S500 pins had higher CV's than did uncoated pins.

Follow this link for more data on liquid to dry transfers.