SBS Registration Feature

     
 


The VP 581A SBS Registration block has the same foot print as a micro-plate (127.7 mm by 85.5 mm and stands 18 mm tall) and is made from polypropylene.  Pre-drilled and taped (8-32) holes in the block provide a simple way to mount/register many accessories to a robot platform deck tile location.

The VP 581B is identical to the VP 581A except it is 12.5 mm tall

The VP 581B-PTFE identical to the VP 581B except it is constructed from PTFE. 

 

 

 
 


The VP 581A registration blocks have many applications.  The plate registration features on the deck tiles of some robotic platforms interfere with the inlet or outlet nipples on the VP 549ETOH or VP 549H2O spillway reservoirs.  We have solved this problem by placing 4 alignment holes in the bottom of the reservoirs (arrows).  The photo to the right illustrates the bottom view of the reservoir and the top view of a VP 581A SBS locating block.  The alignment holes register to 4 set screws in our VP 581A.  Thus we are able to stack the reservoir on top of the SBS locating block and elevate the reservoir above any obstruction.

 
 
 


The photo to the right illustrates the reservoir stacked on top of the SBS locating block.

 

 
 


Modification of Bubble Paddle Reservoirs to Attach the SBS Footprint

 
 


All of our Bubble Paddle Reservoirs can be mounted on a polypropylene block with SBS microplate dimensions (VP 581B).  Mounting is done by drilling and tapping holes in the bottom of the reservoir.  When ordering your reservoir also order VP 581B with modification and your reservoir will be attached to a VP 581B SBS feature.  This will allow your Bubble Paddle Reservoir to be placed in the portrait position of the following Tumble Stirrers VP 710C-1, VP 710C1-7, VP 710G6, VP 710MXL7T, VP 710MXM7T

The advantages of the SBS feature on the bottom of the reservoir is two fold: 

1. It will the position the reservoir so a robotic pipettor will be able to place the pipet tips in the correct locations between the bubble paddles. 

 

2.  The reservoir can be taken off the deck and replaced in exactly the same position.

 
 


  Orbital Shakers with VP 581B-1536

     
 


We offer 1,536 microplate (VP 326) H+P orbital shakers for use on Robot decks. 
The VP 581B-1536 is screwed onto the base of the orbital shaker and used attach the .  

 
 

  Heating Block with VP 581B-MICA

     
 


Designed to meet the high temperature requirements of scientists to heat plates on a robot deck.
  The "cut away" low profile design allows the robot plate handler to move the plates around the deck between multiple heating stations and cooling stations.  To accommodate the 200C heat  of the VP 741BW we recommend the VP 581B-MICA because Very High Temperature Mica "Muscovite Mica"  is able to accommodate higher temperatures than Polypropylene or PTFE. 

 
 
 
The photo on the right illustrates a VP 581A adaptor being used to fixture a 100 C VP 741 heating block to an FXALP-PM.
 
 


  96/384 Plate Reformattor with VP 581B

     
 


VP 381O
is simple registration device that allows the researcher to reformat plates from 96 to 384 or the reverse using a 96 Pin Tool.  The reformattor has a VP 581B SBS footprint on the bottom that fits into a deck station on a robot deck.   When a 384 well microplate is placed on 1 of the 4 positions of the Reformattor, the 96 pin tool or pipetor will be aligned to the same relative quadrant of the microplate.  The entire 384 well microplate is addressed when the 384 well microplate is manually moved between the 4 positions

 
 
 


One of the problems working with PCR Plates is that often they are warped.  This is especially true after they have been through many heating cycles in a thermocycler. Warped plates present several practical problems for pipetting or pin tool applications as corner wells are higher than center wells and well center to center spacing is also affected.  Warped PCR plates can also cause disastrous crashes of automated liquid handling robots.

A customer sent us some  PCR plates that were warped and asked if we could solve the problem.  The VP 581 Plate Flattener and the VP 581A Adapter Block are simple but effective solutions.

 
 


  VP 581

     
 
The VP 581 is designed with a groove in 3 sides that capture the lip of a PCR plate.  Simply slide the plate in the groove from the open (4th) side and the plate is flattened.  The VP 581 Plate Flattener can be attached to the robot deck via 3 screw holes that are drilled on 50.8 mm centers.  This is the same spacing as on the PerkinElmer Multi Probe deck shown in the photo.  

An alternative to screwing the Plate Flattener into the deck is to attach it to the VP 581A Adaptor Block using the same 3 screw holes.   Its dimensions are 127.7 mm long by 85.54 mm wide and 18. mm tall.

The photo to the right illustrates how the VP 581 is attached to a VP 581A Adaptor Block and then  fits onto a Multi Probe microplate holder.  By using this simple Adaptor Block you can locate the flattened PCR plate any where on the robot deck that has a plate holder.  This system is also compatible with many robot plate handlers.

The VP 581 was designed to flatten the Robbins Scientific CyclePlate® -384DW Thin Wall PCR plates which have a 3.16 mm thick lip.  PCR plates with thicker or thinner lips can also be accommodated with some simple modifications.  Send us a sample plate and we will provide a custom solution for you.

Another application for the VP 581 is to provide a very precise registration for 1536 well microplates.  With these plates there is no place for "wiggle room". 

 

 
 
The VP 581A adapter can also be used to mount and locate Bubble Paddle Stirrers to workstation plate holders.  Follow this link to see another example.
 



 


VP 581R-PE

     
 


The VP 581R-PE was designed so the bottom has same dimensions as a microplate (SBS)and top flares out for a pocket that can accommodate a microplate.  We made it to accommodate some of our wash stations that had tubing connectors that interfered with the robot deck plate holders.  This design places the tubing connectors above the obstructions on the plate holders.

 
 
  The VP 581R-PE was designed to be used on PerkinElmer workstation decks such as the EP3 and Janus  
 
     
 


VP 581A-MICA

     
 


The VP 581A-MICA was designed to attach to the bottom of our heat blocks and locate the heat block in a microplate holder on a robotic deck.  The heat blocks are attached to the VP 581B-MICA so that the microplates they hold, will be in the same relative position as if they placed in the holder on a robotic deck.  Thus the robot will not have to be reprogrammed to find the correct X and Y positions. 

 
 
 


The VP 581A-MICA is made from Muscovite, a very high temperature stable Mica which is dimensionally stable over a temperature range of -80C to 500C.  This is a very important characteristic as high temperatures can induce swelling of polypropylene and PTFE material to the point where they won't fit in the microplate holders on the robot deck.  This heat induced swelling of material is a problem for our polypropylene and PTFE versions of the VP 581A and the reason we developed the VP 581A-MICA.  We recommend this product over the other materials we have used for heat blocks in the past.

 

The Muscovite Mica is also a good insulator and a way to prevent or reduce heat from the heat block transferring to  the robot deck.   The Muscovite Mica is safety rated by UL laboratories as UL 94VO.   

 

     


VP 581B-MICA

     
 


The VP 581B-MICA is almost identical to the VP 581A-MICA except in the thickness.  The VP 581B-MICA is 6 mm shorter than the VP 581A-MICA.  This facilitates applications where the Z height is limited.  Also the shorter height translates to a lower price.

 

The VP 581B-MICA was designed to attach to the bottom of our heat blocks and locate the heat block in a microplate holder on a robotic deck.  The heat blocks are attached to the VP 581B-MICA so that the microplates they hold, will be in the same relative position as if they placed in the holder on a robotic deck.  Thus the robot will not have to be reprogrammed to find the correct X and Y positions. 

 
 
 

The VP 581B-MICA is made from Muscovite, a very high temperature stable Mica which is dimensionally stable over a temperature range of -80C to 500C.  This is a very important characteristic as high temperatures can induce swelling of polypropylene and PTFE material to the point where they won't fit in the microplate holders on the robot deck.  This heat induced swelling of material is a problem for our polypropylene and PTFE versions of the VP 581B and the reason we developed the VP 581B-MICA.  We recommend this product over the other materials we have used for heat blocks in the past. 

The photo on the right illustrates the VP 581B-MICA on a VP 741BW-R-MICA low profile heat block.

 
 
 


The Muscovite Mica is also a good insulator and a way to prevent or reduce heat from the heat block transferring to  the robot deck.   The Muscovite Mica is safety rated by UL laboratories as UL 94VO. 

 

The photo on the right illustrates the VP 581B-MICA on a VP 741A-R low profile heat block.

 
 


VP 581R

 
 


The VP 581R is a polypropylene locating block with a SBS footprint.

 
 
 


Photo to the right shows the VP 581R with a VP 549ETOH on top.

 
 
 


The photo to the right shows the VP 581R aligned on a robotic deck.

 
 
       
 

VP 581R-HL

 
 

VP 581R-HL is a SBS-sized microplate adapter with microplate holder on top, straight sides and 4 holes for attaching to the support legs of a Hamilton Multiflex microplate carrier.


When using a V&P 384 Well Microplate Magnetic Bead Separation Block VP 771G-4AM-1 it is recommended that the Registration Base is used with the MagSep Block in order to register the microplate wells to the magnets. The weight of the Separation Block with Registration Base is such that the standard plate holder on a Hamilton Multiflex microplate carrier cannot hold MagSep Block in a secure position for accurate pipetting. This can be an issue when removing liquid from a magnetic bead pellet that may be in close proximity to the pipette tip, such as in a 384 well plate.


V&P Scientific developed the adapter, VP 581R-HL, to hold the VP 771G-4AM-1 securely in place on a Multiflex microplate carrier. The adapter replaces the microplate holdert of the carrier and provides a tight-fitting SBS pocket for the Magnetic Bead Separation Block.

 


 
 

Two VP 581R-HL Adapters are shown on a Hamilton Multiflex Microplate Carrier. The SBS feature of the Registration Base of the VP 771G- 4AM-1 Magnetic Bead Separation Block fits securely in pocket of VP 581R-HL Adapter.

 
 
 

Assembly of VP 581R-HL Adapter onto Hamilton Multiflex Carrier Base using the Multiflex Carrier’s support legs (photos on left below). The VP 581R-HL replaces the microplate holder of the Multiflex Carrier. Below right, a Hamilton Multiflex Carrier Base with two VP 581R-HL Adapters holding two VP 771G-4AM-1 Magnetic Bead Separation Blocks can be seen.

     

 
 

The SBS pocket of VP 581R-HL Adapter can be used to hold a micoplate or any other SBS-dimensioned labware.

 


 
         


VP 581C-AL

     
 


The VP 581C-AL was designed to keep the contents of a microplate cool while on a robotic work station.  Made from block of solid aluminum 2.54 cm thick the
VP 581C-AL will keep a microplate between 7C and 10C for over 15 minutes. 

 

Follow this link to data showing the ability of the VP 581C-AL to stabilize the temperature of a 96 well microplate with 300 ul of distilled water in each well.  The VP 581C-AL were either chilled to 7.8C or -13C prior to placing on the robot deck.

 
 
     
 


VP 581-VPREP

     
 


The VP 581-VPREP was designed to provide a more precise deck microplate holder for the Velocity 11 VPREP now Agilent Vertical work station.  The original VPREP microplate holder provided a loose fit with some pipet tip boxes so we made an accessory that would fit snugly in the VPREP deck holder and would accommodate the tip boxes precisely.

 

 


VP 581-PE

     
 


VP 581-PE is included with the VP 710C1-M which allows a Tumble Stirrer to be precisely positioned on the deck of many different robot platforms (including the Tecan Evo, Caliper Sciclone, Hamiliton Microlab, Star and many others).

 
 


VP 581-PE-2

     
 


The VP 581-PE-2 was designed to attach to the deck of a PerkinElmer EP3 or Janus robot using the two locating pins on the bottom of the VP 581-PE-2 and the locating holes in the both the EP3 and Janus decks.  A variety of accessories can then be attached to the  VP 581-PE-2.

 

The  photo to the right illustrates how a VP 761M coupled with a
VP 756CM-P0 is attached.

 
 
 


Bottom view of a VP 581-PE-2 is illustrated in the photo to the right

 
 



  Modification of Bubble Paddle Reservoirs to Attach the SBS Footprint

 
 


All of our Bubble Paddle Reservoirs can be mounted on a polypropylene block with SBS microplate dimensions (VP 581B).  Mounting is done by drilling and tapping holes in the bottom of the reservoir.  When ordering your reservoir also order VP 581B-1 and your reservoir will be attached to a VP 581B SBS feature.  This will allow your Bubble Paddle Reservoir to be placed in the portrait position of the following Tumble Stirrers VP 710C-1, VP 710C1-7, VP 710G6, VP 710MXL7T, VP 710MXM7T

The advantages of the SBS feature on the bottom of the reservoir is two fold: 

1. It will the position the reservoir so a robotic pipettor will be able to place the pipet tips in the correct locations between the bubble paddles. 

 

2.  The reservoir can be taken off the deck and replaced in exactly the same position.

 
 


  CUSTOMER E-MAIL/COMMENTARY

     
 


Patrick:

I saw the Robbin's 384 well plate holder on the VP site.  I hope you sell a lot of them!  They have worked fantastic for us!  They hold a lot of plates not just the Robbins.  And even non-warped plates are positioned much more precisely than they would be otherwise.  I imagine they would be useful for 1536 and higher plates for which the positioning tolerances are very low!!

Tony Long
Ecology and Evolutionary Biology
Steinhaus Hall
University of California at Irvine
Irvine, CA
92697-2525

     
   

SBS Registration Blocks & Accessories

Robbins Scientific CyclePlate® -384DW Thin Wall PCR plate Flattener VP 581
Polypropylene Registration Block with SBS footprint 18 mm tall VP 581A
Polypropylene Registration Block with SBS footprint 12.5 mm tall VP 581B
PTFE Registration Block with SBS footprint 12.5 mm tall VP 581B-PTFE
 
         

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