A Cost-Effective Way To Pipet 384 Samples Simultaneously

  John Herich Manager-HTS Maxim Pharmaceutical

Presented at the San Diego Chapter of the LRIG 9/26/2001

Maxim HTS Introduction
 

• Screen for inhibitors or inducers of apoptosis

• Proprietary substrate specific for activated caspase

• Screens are performed in 384 well format

• Screens are cell based, prefer < 1% DMSO

Current HTS Equipment
 

• Pipetting

  • 96 channel (CyBi Well/Multimek)

  • 4 channel (Multiprobe)

   

• Plate Movers

  • 384 wells (Specrafluor Plus)

   

• Plate Movers

  • Robotic Arm (Platecrane)

  • Stackers (Platestak)

History

Upgrade Path to Simultaneous 384 Pipetting

Disposable Tip Costs

384 Pin Tool Alternative

Pin Tool Methods

HTS Method With Disposable Tips

Pin Tool Method

Test Method

Just Before Pipetting

Dispense

Reset Pins

Results By Plate

Results for a 10 Plate Run

Accuracy

Conclusions

Practical Methods

Acknowledgements