DMSO Liquid To Liquid Transfers With Uncoated Pins

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Pin Type	Diameter		Shape		96 Format (nL)			384 Format (nL)
FP9		0.229 mm		Solid	4	-	10	3	-	8
FP8		0.350 mm		Solid	13	-	26	6	-	18
FP1		0.457 mm		Solid	18	-	43	11	-	31
FP1S6		0.457 mm		6nl slot	24	-	49	15	-	34
FP1S10		0.457 mm		10nl slot	30	-	54	21	-	40
FP1S20		0.457 mm		20nl slot	37	-	61	27	-	46
FP1S30		0.457 mm		30nl slot	46	-	68	35	-	54
FP1S40		0.457 mm		40nl slot	57	-	78	45	-	63
FP1S50		0.457 mm		50nl slot	70	-	90	56	-	75
FP3		0.787 mm		Solid	67	-	139	29	-	79
FP4		0.910 mm		Solid	94	-	197	34	-	98
FP3S100		0.787 mm		100nl slot	175	-	241	114	-	163
FP3S200		0.787 mm		200nl slot	280	-	332	203	-	250
FP3S500		0.787 mm		500nl slot	535	-	559	427	-	464
FP4S1000		0.910 mm		1000nl slot	940	-	1011	704	-	800
FP4S2000		0.910 mm		2000nl slot	1518	-	1608	1277	-	1362

FP		1.580 mm	Solid Pointed		147	-	411	168	-	395
FPS.5		1.580 mm		0.5ul slot	442	-	704	631	-	843
FPS		1.580 mm		1ul slot	893	-	1130	1343	-	1498
FPS2		1.580 mm		2ul slot	1911	-	2038	2607	-	2767
FPS5		1.580 mm		5ul slot	3908	-	4296	5180	-	5253

FP6		1.580 mm		Solid Flat	323	-	674	154	-	398
FP6S.5		1.580 mm		0.5ul slot	734	-	1042	855	-	1053
FP6S		1.580 mm		1ul slot	1210	-	1500	1638	-	1717
FP6S2		1.580 mm		2ul slot	2299	-	2384	2787	-	3068
FP6S5		1.580 mm		5ul slot	4329	-	4656	5237	-	5245

*# delivery volume range is determined by speed of withdrawal from source liquid.
slow speed = 0.78 cm/sec = low volume delivery range
fast speed = 5.70 cm/sec = high volume delivery range
Follow this link to assay method

*Speed of withdrawal determines volume transferred. The faster the withdrawal speed the larger the volume transferred. Withdrawal speed range is from 1.5 mm/sec to 30mm/sec.

*The volume of liquid in the source well also makes a significant difference in the volume transferred and there is a difference between sold and slotted pins. The volume used for the 96 well data was 200 ul and the volume used for the 384 well data was 75 ul. If you use more or less volume in your wells it will affect the volume transferred. See this link for more information or See this link for more data.