48 Pin Multi-Blot Replicator

     


  48 Pin Tools for 48 well Microplates on 13.68 mm centers

     
 
VP 480
     
 
48 stainless steel pins in an anodized aluminum base. Compatible with  many 48 well plates. The pins transfer ~2 ul liquid to liquid, and ~.2 ul liquid to membrane (hanging drop). Also for use in transferring liquids to agar plates.  Pin diameter = 1.58mm, pin length = 21.8mm, center to center spacing = 13.68 mm 

Check the center to center spacing on your plate very carefully as this varies between manufacturers.  Also the location of the matrix (position of the A1 well) varies between manufacturers.

If you are transferring DNA, cDNA or other materials in hydrophobic solutions you will find that by using our new V&P Pin Cleaning Solution you  will greatly increase the precision of delivery.  For an explanation see the FAQ page and the V&P Pin Cleaning Solution page.
 

 
 
 

 
  Technical Documents:
    Care and use of Multi-Blot Replicator
 

 

  48 Pin Tools for 48 well Microplates on 13 mm centers

     
 


VP 481-P12

     
 


48 stainless steel pins in an anodized aluminum base. Compatible with Falcon # 3078, Greiner 677180 CellStar 48W and Costar  48 well plates. The pins transfer ~1.5 ul liquid to liquid, and ~0.75 ul liquid to membrane (hanging drop). Also for use in transferring liquids to agar plates.  Pin diameter = 2.36mm, pin length = 21.8mm, center to center spacing = 13.08 mm

Check the center to center spacing on your plate very carefully as this varies between manufacturers.  Also the location of the matrix varies between manufacturers.  Nunc 48 well plates are on 13.5 mm centers.

If you are transferring DNA, cDNA or other materials in hydrophobic solutions you will find that by using our new V&P Pin Cleaning Solution you  will greatly increase the precision of delivery.  For an explanation see the FAQ page and the V&P Pin Cleaning Solution page.
 

 
 
 

 
  Technical Documents:
    Care and use of Multi-Blot Replicator
 



VP 481M

     
 


48 Magnetic stainless steel pins in an anodized aluminum base. Compatible with Greiner (#677180) CellStar (48W), Falcon (#3078) and Costar  48 well plates (#3548). Although designed to remove stir elements from 48 well microplates, the pins will also transfer ~1 ul liquid to liquid, and ~0.2 ul liquid to membrane (hanging drop). Also for use in transferring liquids to agar plates.  Pin diameter = 1.58mm, pin length = 21.8mm, center to center spacing = 13.08 mm

Check the center to center spacing on your plate very carefully as this varies between manufacturers.  Also the location of the matrix varies between manufacturers.  Nunc 48 well plates are on 13.5 mm centers.

If you are transferring DNA, cDNA or other materials in hydrophobic solutions you will find that by using our new V&P Pin Cleaning Solution you  will greatly increase the precision of delivery.  For an explanation see the FAQ page and the V&P Pin Cleaning Solution page.
 

 
 
 

 
  Technical Documents:
    Magnetic Stirrer Loader & Unloader Manual
 



VP 481-P13

     
 

48 stainless steel pins in a Ni-Lube coated aluminum base.

Compatible with Greiner (#677180) CellStar (48W), Falcon (#3078) and Costar  48 well plates (#3548). Although designed to remove stir elements from 48 well microplates, the pins will also transfer ~1 ul liquid to liquid, and ~0.2 ul liquid to membrane (hanging drop). Also for use in transferring liquids to agar plates.  Pin diameter = 3.18mm, pin length = 21.8mm, center to center spacing = 13.08 mm.

Check the center to center spacing on your plate very carefully as this varies between manufacturers.  Also the location of the matrix varies between manufacturers.  Nunc 48 well plates are on 13.5 mm centers.

If you are transferring DNA, cDNA or other materials in hydrophobic solutions you will find that by using our new V&P Pin Cleaning Solution you  will greatly increase the precision of delivery.  For an explanation see the FAQ page and the V&P Pin Cleaning Solution page.
 

 
 
 


  48 Pin Tools for 96 and 384 well Microplates on 9 mm centers

 


VP 408H

     
 


The VP 408H is very convenient for inoculating 85 mm diameter Petri dishes and membranes with yeast or other microorganisms. Also for use in colony to colony transfers on agar or other surfaces.  It is also compatible with 384 well microplates.  It is made with 48 stainless steel pins of the same length pressed into an anodized aluminum base to facilitate a flat contact.  It can be alcohol flame sterilized or autoclaved.  The pins are on 9 mm centers, (96 well format) 1.58mm in diameter, 22mm long  and carry a hanging drop of ~250 nl
 

 
 
 

 
  Technical Documents:
    Care and use of Multi-Blot Replicator
 



VP 408HA

     
 


The VP 408HA is very convenient for inoculating 85 mm diameter Petri dishes and membranes with yeast or other microorganisms. Also for use in colony to colony transfers on agar or other surfaces.  It is also compatible with 384 well microplates.  It is made with 48 stainless steel pins of the same length pressed into an anodized aluminum base to facilitate a flat contact.  It can be alcohol flame sterilized or autoclaved.  The pins are on 9 mm centers, (96 well format) 1.58mm in diameter, 57mm long  and carry a hanging drop of ~250 nl
 

 
 
 

 
  Technical Documents:
    Care and use of Multi-Blot Replicator
 



VP 407AH

     
 

The VP 407AH is the preferred pin tool for Yeast researchers who want to inoculate larger volumes (~3 ul) onto an agar Petri dish.   Also for use in colony to colony transfers on agar or other surfaces. The pins are spaced to fit into half of a 96 well microplate yet are small enough to inoculate a 85 mm Petri plate.   It is made with 48 stainless steel pins of the same length pressed into an anodized aluminum base to facilitate a flat contact.   It can be alcohol flame sterilized or autoclaved.  The pins are on 9 mm centers, (96 well format) 3.15mm in diameter, 22mm long  and carry a hanging drop of ~3 ul


Testimonial from postdoctoral fellow at a major university: "....regarding the product VP 407AH. I have been using this pin tool and I think it is fantastic."

See our floating pin tools for transferring yeast and bacteria to agar Omni Trays.
 

 
 
 

 
  Technical Documents:
    Care and use of Multi-Blot Replicator
 

 


VP 409-48

     
 
The Replicator to the right is a VP 409 with every other column filled with pins.  This design allows the customer to inoculate only one half the plate at a time.  The other half of the plate can be addressed by merely taking the VP 381 Library Copier off and rotating it 180 degrees.  What ever your purpose, assay or control well scheme is – we have a solution for you.
 
 
     
 

 

  Blot Type Replicators

     
 


VP 404A

     
 


48 stainless steel bolts on 9 mm centers (96 well format) with an anodized aluminum base. The bolts pick up liquid in the threads, transferring up to ~10 ul of liquid in the bolt threads (depending on the depth of submersion of the bolts).     Not for use in transferring liquids to agar  plates or membranes,  or colony to colony transfers on agar due to the irregularity in bolt length.  Bolt length = ~17.7mm, bolt diameter = 3.4mm

Click here to see more varieties of Bolt Replicators
 

 
 
 

 
  Technical Documents:
    Care & Use of Bolt Replicators
 



  Floating Pin Replicators

     
 


AFIX48FP13M

     
 
The AFIX48FP13M was designed to transfer yeast or bacterial cultures from a 96 well microplate to an agar petri dish.

FP13 pins sold separately
Flat tip, 3.18 mm diameter
Exposed pin length = 22 mm
Overall pin length = 27 mm
Solid pin hanging drop = ~3 ul

BMPM-E-CLIP
Manual mounting plate and handle sold separately

 
 


AFIX48FP

     
 
The AFIX48FP was designed to make wounds in tissue culture monolayers grown in 48 well microplates.  Each of the pins float the fixture and has a silicone rubber gasket to press against to keep all the pins at the same level.

FP pins sold separately
Pointed tip, 0.38 mm diameter at the tip with a 1.58 mm diameter shaft.
Exposed pin length = 22 mm
Overall pin length = 34 mm
 

BMPM-E-CLIP
Manual mounting plate and handle sold separately.

BMPM-PAD
silicone rubber gasket sold separately

 

 
 


  Cleaning Pins

     
 


The most important aspect of using pin tools is to start with clean pins and then keep them clean so they will consistently deliver the same volume.  We recommend our Pin Cleaning Solution (VP 110) to clean the pin of protein and other residue buildup before you start an assay.  To quickly and efficiently remove the previous transfer from the pin we have a wide variety of manual and robot wash reservoirs ranging from disposable polypropylene reservoirs (VP 576D) to reusable wash reservoirs, flowing reservoirs and fountains to clean  96, 384 and 1536 pin tools.  The robot wash stations are also adapted so the blotting station is part of the washing station thus reducing the cleaning cycle time and the number of robot stations required for the cleaning process.  We also have other independent blotting stations (VP 540D) and VP 522.

Integral to the cleaning process of all pins is the blotting step between baths.  Lint particles in the slots or on pin tips, from standard paper towels, will interfere with the loading and unloading of liquid.  We have solved this problem.  The VP 540DB blotting system is a tray with the standard microplate foot print that contains a super absorbent polypropylene pad covered by a Lint Free Blotting Paper.  We strongly recommend that you use this or our other lint free blotting systems.

 

 
  Click here for a list of published articles that cite using the products on this page.  
     

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