Liquid Transfers 1 Mother Plate to 1 Daughter Plates

  Initial Wash

·         move pin cleaning solution over the lifting stage and raise it until the pin tips touch the bottom of the well

·         hold in this position for 30 sec

·         move the stage down so the pins are just barely out of the pin cleaning solution

·         move the stage up so the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the pin cleaning solution

·         move the stage up so the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the pin cleaning solution

·         move the stage up so the pin tips touch the bottom of the well

·         change the speed of the stage to around 0.7cm/s

·         move the stage down to 0 position

·         move the blotting paper over the stage

·         move the blotting paper up to touch the pins at half speed

·         move water  over the lifting stage and raise it until the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the water

·         move the stage up so the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the water

·         move the stage up so the pin tips touch the bottom of the well

·         change the speed of the stage to around 0.7cm/s

·         move the stage down to 0 position

·         move the blotting paper over the stage

·         move the blotting paper up to touch the pins at half speed

·         move EtOH  over the lifting stage and raise it until the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the EtOH

·         move the stage up so the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the EtOH

·         move the stage up so the pin tips touch the bottom of the well

·         change the speed of the stage to around 0.7cm/s

·         move the stage down to 0 position

·         move the blotting paper over the stage

·         move the blotting paper up to touch the pins at half speed

·         move the blotting paper down

  Liquid Transfer

·         get mother plate 1 from Stack B

·         move mother plate 1 over lifting stage

·         raise mother plate 1 so pins touch the bottom of the plate

·         move the stage down so the pins are just barely out of the liquid

·         move the stage up so the pin tips touch the bottom of the plate

·         move the stage down so the pins are just barely out of the liquid

·         move the stage up so the pin tips touch the bottom of the plate

·         change the speed of the stage to around 0.7cm/s

·         move the stage down to 0 position

·         put mother plate 1 back in stack B

·         get daughter plate 1 from stack A

·         raise daughter plate 1 so pins touch the bottom of the plate

·         move the stage down so the pins are just barely out of the liquid

·         move the stage up so the pin tips touch the bottom of the plate

·         move the stage down so the pins are just barely out of the liquid

·         move the stage up so the pin tips touch the bottom of the plate

·         change the speed of the stage to around 0.7cm/s

·         move the stage down to 0 position

·         put daughter plate 1 back in stack A

  Clean off Pins

·         move water  over the lifting stage and raise it until the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the water

·         move the stage up so the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the water

·         move the stage up so the pin tips touch the bottom of the well

·         change the speed of the stage to around 0.7cm/s

·         move the stage down to 0 position

·         move the blotting paper over the stage

·         move the blotting paper up to touch the pins at half speed

·         move EtOH  over the lifting stage and raise it until the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the EtOH

·         move the stage up so the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the EtOH

·         move the stage up so the pin tips touch the bottom of the well

·         change the speed of the stage to around 0.7cm/s

·         move the stage down to 0 position

·         move the blotting paper over the stage

·         move the blotting paper up to touch the pins at half speed

  Liquid Transfer

·         get mother plate 2 from stack B

·         move mother plate 2 over lifting stage

·         raise mother plate 2 so pins touch the bottom of the plate

·         move the stage down so the pins are just barely out of the liquid

·         move the stage up so the pin tips touch the bottom of the plate

·         move the stage down so the pins are just barely out of the liquid

·         move the stage up so the pin tips touch the bottom of the plate

·         change the speed of the stage to around 0.7cm/s

·         move the stage down to 0 position

·         put mother plate 2 back in stack B

·         get daughter plate 2 from stack A

·         raise daughter plate 2 so pins touch the bottom of the plate

·         move the stage down so the pins are just barely out of the liquid

·         move the stage up so the pin tips touch the bottom of the plate

·         move the stage down so the pins are just barely out of the liquid

·         move the stage up so the pin tips touch the bottom of the plate

·         change the speed of the stage to around 0.7cm/s

·         move the stage down to 0 position

·         put daughter plate 2 back in stack A

  Final Wash

·         move pin cleaning solutionover the lifting stage and raise it until the pin tips touch the bottom of the well

·         hold in this position for 30 sec

·         move the stage down so the pins are just barely out of the pin cleaning solution

·         move the stage up so the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the pin cleaning solution

·         move the stage up so the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the pin cleaning solution

·         move the stage up so the pin tips touch the bottom of the well

·         change the speed of the stage to around 0.7cm/s

·         move the stage down to 0 position

·         move the blotting paper over the stage

·         move the blotting paper up to touch the pins at half speed

·         move water  over the lifting stage and raise it until the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the water

·         move the stage up so the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the water

·         move the stage up so the pin tips touch the bottom of the well

·         change the speed of the stage to around 0.7cm/s

·         move the stage down to 0 position

·         move the blotting paper over the stage

·         move the blotting paper up to touch the pins at half speed

·         move EtOH  over the lifting stage and raise it until the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the EtOH

·         move the stage up so the pin tips touch the bottom of the well

·         move the stage down so the pins are just barely out of the EtOH

·         move the stage up so the pin tips touch the bottom of the well

·         change the speed of the stage to around 0.7cm/s

·         move the stage down to 0 position

·         move the blotting paper over the stage

·         move the blotting paper up to touch the pins at half speed

·         move the blotting paper down