1536 Pin Replicators

     

 
  Solid Pin

     
 
  • Add or Transfer 1536 Samples

  • Initiate 1536 Assays Simultaneously

  • Test 1536 Compounds

  • Add Substrate to 1536 Assays Simultaneously

     
 

With the advent of the 1536 well microplates by Greiner Labortechnik, Corning and now Nalge Nunc, V&P Scientific has launched the first stainless steel 1536 Pin Replicators to transfer nl volumes of liquids to and from these plates. The VP 838 Replicator has 0.457 mm diameter stainless steel pins with flat tips that deliver ~20 nl in a liquid to liquid transfer. (For liquid to membrane or dry plate, smaller volumes will be transferred from the hanging drop on the pin tip, see complete explanation on the FAQ page)

We have also developed a new spring loaded Registration Frame and Replicator Housing System (VP 836A) that allows the user to register 1536 Pin Replicators (VP 838) to a LIBRARY COPIER™ for the Nalge Nunc and Greiner 1536 microplates (VP 881), another for the Corning 1536 microplate (VP 881A), and another LIBRARY COPIER™ to register the Replicators to glass plates or nylon membranes (VP 832) for blotting or spotting. This precise registration system ensures that the Replicator pins are centered in each of the wells and onto precise locations on membranes or glass plates.

Click here for a list of published articles that cite using the products on this page.

 

 
   
Registration Frame and Replicator Housing System for 1536 Pin Replicator (VP 838) - spring loaded VP 836A
1536 Pin Replicator, ~20 nl delivery volume, delrin base with stainless steel pins, pin length = 14 mm, pin diameter = 0.457 mm VP 838
LIBRARY COPIER™ to register Replicator to the large volume (8 to 12 ul) 1536 Nalge Nunc, Corning or Greiner microplates VP 881
LIBRARY COPIER™ to register Replicator to the 2 ul well 1536 Corning microplate VP 881A
LIBRARY COPIER™ to register Replicator to glass plates and nylon membranes. The position on the plate or membrane is stabilized with double sided sticky tape on the bottom of the LIBRARY COPIER™ VP 882
Hybond N nylon membranes, 10cm x 15cm VP 502
   
 


This blot was made using the VP 838 Replicator transferring ~20 nl of specimen to a nylon membrane.

   


  Floating 1536 Pin Replicators

     
 


The stainless steel pins in the VP 838FP Series Replicators come in diameters of 0.229 mm (FP9), 0.457mm (FP1) and 0.787mm (FP3) and will deliver ~4 nl, ~20 nl and ~80 nl respectively in liquid to liquid transfers. These small pin diameters eliminates "well wall clearance" problems encountered with larger diameter pins with some brands of 1536 well microplates where the center to center spacing of the wells has shrunk in the molding process.

Each 50.8mm long pin is fixtured inside a 1.58mm diameter tube that is 31.75mm long, thus strengthening the pin design and adding mass to enhance the floating characteristics.  Each pin slides ("floats") through holes in the lower and upper float plates as it touches on the bottom of the microplate well or the gridding surface.  This floating action assures that each of the pins will touch and deliver its droplet even if the microplate is warped or the blotting/gridding surface is not level or smooth.

The VP 838FP series Replicators are designed to be used manually or with a robotic work station. Just fax us a drawing of the mating feature on your robot and we will incorporate that design into the mating plate.

 


The 25.4 mm alignment distance between the lower and upper float plates insures that pin to pin spacing remains true at 2.25 mm. The evenness of this spacing is demonstrated in the blot to the right.

 

 


  High Throughput Library Copiers™ for 1536 Floating Pin Replicators

 
 


The VP 381F  LIBRARY COPIER™ was designed for High Throughput with the 96, 384 and 1536 pin  FP series Floating Pin Replicators as these floating pin replicators have shorter pins they need a shorter LIBRARY COPIER™.   The open design of the VP 381F allows the operator ease of access to place and remove microplates.

The VP 381F LIBRARY COPIER™ was also designed to accommodate 2 ul Corning 1536 microplates.  These plates are easily accommodated by larger opening of the VP 381F.  The VP 381F also can be used with all of the 1536 FP1, FP3 and FP9 series MULTI-BLOT™ Replicators as a high throughput LIBRARY COPIER™.

 
 
 
 

1536 Pin Replicators

 
1536 pins, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter= 0.457 mm, Replicator height = 1800 grams, delivery volume = ~20 nl 
VP 838FP1
1536 pins, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter=0.787mm,  Replicator height = 1860grams, delivery volume = ~80 nl  VP 838FP3
1536 pins, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter= 0.229 mm, Replicator height = 1760 grams, delivery volume = ~4nl  VP 838FP9

1536 Slot Pin Replicators

 
1536 pins, slot volume = 10 nl, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.457 mm, Replicator weight = 1800 grams VP 838FP1S10
1536 pins, slot volume = 50 nl, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.457 mm, Replicator weight = 1860 grams  VP 838FP1S50
1536 pins,  slot volume = 100 nl, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.787 mm, Replicator weight =1860 grams VP 838FP3S100
1536 pins,  slot volume = 200 nl, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.787 mm, Replicator weight =1860 grams VP 838FP3S200
1536 pins,  slot volume = 500 nl, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.787 mm, Replicator weight =1860 grams VP 838FP3S500
 


  Accessories

     
 
LIBRARY COPIER™ to register VP 838FP Series Replicators to large volume (8 to 12 ul wells)1536 Nalge Nunc, Corning or Greiner microplates VP 381N
LIBRARY COPIER™ to register VP 838FP Series Replicators to 2 ul well size 1536 Corning microplates VP 381F
Conventional MULTI-PRINT™ to register VP 838FP Series Replicators to glass plates and nylon membranes. The position on the plate or membrane is stabilized with double sided sticky tape on the bottom of the MULTI-PRINT™ VP 382
Hybond N Nylon Membranes, 10 cm x 15 cm VP 502
 


  Selection of System

     
 


You need to choose whether you will be using the fixed pin system (VP 838) or the floating pin system with or without a robotic work station (VP 838FP Series Replicator).  If you select the VP 838 you will need to determine which 1536 microplate you will be using (Nalge Nunc, Greiner or Corning) so you can select one or more of the appropriate LIBRARY COPIERS to register the Replicator to the microplate (VP 881 - for Nalge Nunc and Greiner microplates or VP 881A - Corning  microplates).  If you want to make blots on membranes or glass then include the VP 882 and you have a complete system.  To use the VP 838FP Series Replicators manually you will need to purchase a LIBRARY COPIER™ to register the replicator to microplate of your choice, VP 381N for Nalge Nunc and Greiner and VP 381F for Corning microplates.  The VP 838FP Series Replicators can be used with a VP 382 to make blots on membranes.  

As more microplate manufactures introduce 1536 well microplates we will make LIBRARY COPIERS™ to register both the fixed and Floating Replicator versions. 

 
 
  Hydrophobic & Lipiphobic Coating of Pins
     
 


All of the above pins can also be obtained with a hydrophobic/lipophobic coating to reduce non-specific binding by proteins and lipoproteins.  Just adding an H to the above pin designates that pin as a hydrophobic/lipophobic coated pin.  This coating is metallic and only a few angstroms thick so it does not effect the basic dimensions of the pins.  These coated pins are very useful in handling solutions with proteins or lipids that may stick to naked stainless steel.  Click on the following hyperlink for more details on the nature of our hydrophobic/lipophobic coating and the coating process.

 
 


Hydrophobic Pins used in the Standard Double Float Plate Replicators 

 
 
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.787 mm, delivery volume = ~80 nl, Hydrophobic coated
FP3H
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.457 mm, delivery volume = ~20 nl, Hydrophobic coated
FP1H
Exposed pin length = 17 mm, overall pin length = 50.8 mm, pin diameter = 0.229 mm, delivery volume = ~4 nl, Hydrophobic coated
FP9H
 
 


1536 Hydrophobic Pin Replicators

     
 
1536 pins, hydrophobic coated, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.457 mm, Replicator weight = 1800 grams, delivery volume = ~20 nl 
VP 838FP1H
1536 pins, hydrophobic coated, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.787 mm, Replicator weight = 1860 grams, delivery volume = ~80 nl 
VP 838FP3H
1536 pins, hydrophobic coated, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.229 mm, Replicator weight =1760 grams, delivery volume = ~4 nl 
VP 838FP9H
 
 


1536 Hydrophobic Slot Pin Replicators

     
 
1536 pins, hydrophobic coated, slot volume = 10 nl, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.457 mm, Replicator weight = 1800 grams
VP 838FP1S10H
1536 pins, hydrophobic coated, slot volume = 50 nl, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.457 mm, Replicator weight = 1800 grams
VP 838FP1S50H
1536 pins, hydrophobic coated, slot volume = 100 nl, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.787mm, Replicator weight = 1860 grams
VP 838FP3S100H
1536 pins, hydrophobic coated, slot volume = 200 nl, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.787mm, Replicator weight = 1860 grams VP 838FP3S200H
1536 pins, hydrophobic coated, slot volume = 500 nl, height of Replicator = 67 mm, exposed pin length = 17 mm, pin diameter = 0.787mm, Replicator weight = 1860 grams VP 838FP3S500H
 


  Cleaning Pins

     
 


The most important aspect of using pin tools is to start with clean pins and then keep them clean so they will consistently deliver the same volume.  We recommend our Pin Cleaning Solution (VP 110) to clean the pin of protein and other residue buildup before you start an assay.  To quickly and efficiently remove the previous transfer from the pin we have a wide variety of manual and robot wash reservoirs ranging from disposable polypropylene reservoirs (VP 576D) to reusable wash reservoirs, flowing reservoirs and fountains to clean  96, 384 and 1536 pin tools.  The robot wash stations are also adapted so the blotting station is part of the washing station thus reducing the cleaning cycle time and the number of robot stations required for the cleaning process.  We also have other independent blotting stations (VP 540D) and VP 522.

Integral to the cleaning process of all pins is the blotting step between baths.  Lint particles in the slots or on pin tips, from standard paper towels, will interfere with the loading and unloading of liquid.  We have solved this problem.  The VP 540DB blotting system is a tray with the standard microplate foot print that contains a super absorbent polypropylene pad covered by a Lint Free Blotting Paper.  We strongly recommend that you use this or our other lint free blotting systems.

 
 


Click here for a list of published articles that cite using the products on this page.

 
         

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